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Rabbit Polyclonal SGPP1 antibody. Suitable for ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human SGPP1 aa 250-300.

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Images

Immunocytochemistry/ Immunofluorescence - Anti-SGPP1 antibody (AB262889), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Recombinant Fragment Protein within Human SGPP1 aa 250-300. The exact immunogen used to generate this antibody is proprietary information. Database link Q9BX95

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IF
Human
Tested

Tested
Tested

Species

Human

Dilution info

1.00000-4.00000 µg/mL

Notes

Fixation/Permeabilization: PFA/Triton X-100

Associated Products

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Target data

Function

Specifically dephosphorylates sphingosine 1-phosphate (S1P), dihydro-S1P, and phyto-S1P. Does not act on ceramide 1-phosphate, lysophosphatidic acid or phosphatidic acid (PubMed:16782891). Sphingosine-1-phosphate phosphatase activity is needed for efficient recycling of sphingosine into the sphingolipid synthesis pathway (PubMed:11756451, PubMed:12815058, PubMed:16782891). Regulates the intracellular levels of the bioactive sphingolipid metabolite S1P that regulates diverse biological processes acting both as an extracellular receptor ligand or as an intracellular second messenger (PubMed:11756451, PubMed:12815058, PubMed:16782891). Involved in efficient ceramide synthesis from exogenous sphingoid bases. Converts S1P to sphingosine, which is readily metabolized to ceramide via ceramide synthase. In concert with sphingosine kinase 2 (SphK2), recycles sphingosine into ceramide through a phosphorylation/dephosphorylation cycle (By similarity). Regulates endoplasmic-to-Golgi trafficking of ceramides, resulting in the regulation of ceramide levels in the endoplasmic reticulum, preferentially long-chain ceramide species, and influences the anterograde membrane transport of both ceramide and proteins from the endoplasmic reticulum to the Golgi apparatus (PubMed:16782891). The modulation of intracellular ceramide levels in turn regulates apoptosis (By similarity). Via S1P levels, modulates resting tone, intracellular Ca(2+) and myogenic vasoconstriction in resistance arteries (PubMed:18583713). Also involved in unfolded protein response (UPR) and ER stress-induced autophagy via regulation of intracellular S1P levels (PubMed:18583713, PubMed:20798685). Involved in the regulation of epidermal homeostasis and keratinocyte differentiation (By similarity).

Alternative names

Recommended products

Rabbit Polyclonal SGPP1 antibody. Suitable for ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human SGPP1 aa 250-300.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Recombinant Fragment Protein within Human SGPP1 aa 250-300. The exact immunogen used to generate this antibody is proprietary information. Database link Q9BX95
Purification technique

Affinity purification Immunogen

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

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Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

SGPP1 also known as sphingosine-1-phosphate phosphatase 1 is an enzyme that dephosphorylates sphingosine-1-phosphate (S1P) to sphingosine. This enzymatic activity helps regulate the levels of S1P a bioactive lipid involved in various cellular processes. The molecular mass of SGPP1 is approximately 50 kDa. SGPP1 is expressed in a variety of tissues including the liver brain and kidneys indicating its widespread role in the body.

Biological function summary

SGPP1 plays an important role in controlling the sphingolipid signaling pathway influencing cell growth survival and migration. It is not part of a larger complex but operates through interaction with S1P and its receptors. By removing phosphate groups from S1P SGPP1 reduces the signaling activity of this lipid mediator therefore influencing various biological responses including immune cell trafficking.

Pathways

SGPP1 functions within the sphingolipid metabolism pathway which is closely tied to the regulation of cellular processes such as proliferation and apoptosis. Its activity has a strong connection with S1P lyase which further degrades sphingosine. SGPP1 also interacts with enzymes involved in the ceramide synthesis pathway reflecting its role in balancing the levels of different sphingolipids that affect cellular functions.

Associated diseases and disorders

SGPP1 affects conditions such as cancer and inflammation. Its ability to modulate S1P concentrations links it to tumor growth and spread as altered sphingolipid signaling can drive oncogenic processes. An additional association exists with inflammatory disorders where SGPP1 influences immune responses. SGPP1 interacts with SGPL1 another key enzyme in the sphingolipid pathway affecting these disease outcomes.

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1 product image

  • Immunocytochemistry/ Immunofluorescence - Anti-SGPP1 antibody (ab262889), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-SGPP1 antibody (ab262889)

    PFA-fixed, Triton X-100 permeabilized human A-431 ( human epidermoid carcinoma cell line) cells stained for SGPP1 (green) using ab262889 at 4 μg/ml in ICC/IF. The cytoskeketon is counterstained in Red.

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Product protocols

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