Rabbit Polyclonal SGPP1 antibody. Suitable for ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human SGPP1 aa 250-300.
IgG
Rabbit
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Liquid
Polyclonal
ICC/IF | |
---|---|
Human | Tested |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1.00000-4.00000 µg/mL | Notes Fixation/Permeabilization: PFA/Triton X-100 |
Select an associated product type
Specifically dephosphorylates sphingosine 1-phosphate (S1P), dihydro-S1P, and phyto-S1P. Does not act on ceramide 1-phosphate, lysophosphatidic acid or phosphatidic acid (PubMed:16782891). Sphingosine-1-phosphate phosphatase activity is needed for efficient recycling of sphingosine into the sphingolipid synthesis pathway (PubMed:11756451, PubMed:12815058, PubMed:16782891). Regulates the intracellular levels of the bioactive sphingolipid metabolite S1P that regulates diverse biological processes acting both as an extracellular receptor ligand or as an intracellular second messenger (PubMed:11756451, PubMed:12815058, PubMed:16782891). Involved in efficient ceramide synthesis from exogenous sphingoid bases. Converts S1P to sphingosine, which is readily metabolized to ceramide via ceramide synthase. In concert with sphingosine kinase 2 (SphK2), recycles sphingosine into ceramide through a phosphorylation/dephosphorylation cycle (By similarity). Regulates endoplasmic-to-Golgi trafficking of ceramides, resulting in the regulation of ceramide levels in the endoplasmic reticulum, preferentially long-chain ceramide species, and influences the anterograde membrane transport of both ceramide and proteins from the endoplasmic reticulum to the Golgi apparatus (PubMed:16782891). The modulation of intracellular ceramide levels in turn regulates apoptosis (By similarity). Via S1P levels, modulates resting tone, intracellular Ca(2+) and myogenic vasoconstriction in resistance arteries (PubMed:18583713). Also involved in unfolded protein response (UPR) and ER stress-induced autophagy via regulation of intracellular S1P levels (PubMed:18583713, PubMed:20798685). Involved in the regulation of epidermal homeostasis and keratinocyte differentiation (By similarity).
SPP1, SGPP1, Sphingosine-1-phosphate phosphatase 1, SPPase1, Spp1, hSPP1, hSPPase1, Sphingosine-1-phosphatase 1, Sphingosine-1-phosphate phosphohydrolase 1, SPP-1
Rabbit Polyclonal SGPP1 antibody. Suitable for ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human SGPP1 aa 250-300.
IgG
Rabbit
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Liquid
Polyclonal
Affinity purification Immunogen
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
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This supplementary information is collated from multiple sources and compiled automatically.
SGPP1 also known as sphingosine-1-phosphate phosphatase 1 is an enzyme that dephosphorylates sphingosine-1-phosphate (S1P) to sphingosine. This enzymatic activity helps regulate the levels of S1P a bioactive lipid involved in various cellular processes. The molecular mass of SGPP1 is approximately 50 kDa. SGPP1 is expressed in a variety of tissues including the liver brain and kidneys indicating its widespread role in the body.
SGPP1 plays an important role in controlling the sphingolipid signaling pathway influencing cell growth survival and migration. It is not part of a larger complex but operates through interaction with S1P and its receptors. By removing phosphate groups from S1P SGPP1 reduces the signaling activity of this lipid mediator therefore influencing various biological responses including immune cell trafficking.
SGPP1 functions within the sphingolipid metabolism pathway which is closely tied to the regulation of cellular processes such as proliferation and apoptosis. Its activity has a strong connection with S1P lyase which further degrades sphingosine. SGPP1 also interacts with enzymes involved in the ceramide synthesis pathway reflecting its role in balancing the levels of different sphingolipids that affect cellular functions.
SGPP1 affects conditions such as cancer and inflammation. Its ability to modulate S1P concentrations links it to tumor growth and spread as altered sphingolipid signaling can drive oncogenic processes. An additional association exists with inflammatory disorders where SGPP1 influences immune responses. SGPP1 interacts with SGPL1 another key enzyme in the sphingolipid pathway affecting these disease outcomes.
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This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
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PFA-fixed, Triton X-100 permeabilized human A-431 ( human epidermoid carcinoma cell line) cells stained for SGPP1 (green) using ab262889 at 4 μg/ml in ICC/IF. The cytoskeketon is counterstained in Red.
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