AB200346

Anti-SGSH/HSS antibody [EPR17312]

Name: Anti-SGSH/HSS antibody [EPR17312] Rabbit monoclonal (ab200346) | Abcam
Brand: Abcam Limited
SKU: AB200346
Price: 470 USD
Availability: InStock
Rating: 4 (1 reviews)

RabMAb
Recombinant
What is this?

(1 Review)

(2 Publications)

Rabbit Recombinant Monoclonal SGSH/HSS antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 2 publications.

View Alternative Names

HSS, SGSH, N-sulphoglucosamine sulphohydrolase, Sulfoglucosamine sulfamidase, Sulphamidase

5 Images

Immunocytochemistry/ Immunofluorescence - Anti-SGSH/HSS antibody [EPR17312] (AB200346)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SGSH/HSS antibody [EPR17312] (AB200346)

Immunofluorescent analysis of 100% methanol-fixed, 0.1% Triton X-100 permeabilized HepG2 (Human liver hepatocellular carcinoma) cells labeling SGSH/HSS with ab200346 at 1/250 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Cytoplasm staining on HepG2 cell line was observed. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).

The negative controls are as follows :
-ve control 1 : ab200346 at 1/250 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
-ve control 2 : ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SGSH/HSS antibody [EPR17312] (AB200346)

Immunofluorescent analysis of 100% methanol-fixed, 0.1% Triton X-100 permeabilized MCF7 (Human breast adenocarcinoma cell line) cells labeling SGSH/HSS with ab200346 at 1/250 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Cytoplasm staining on MCF7 cell line was observed. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).

Supplier Data

Western blot - Anti-SGSH/HSS antibody [EPR17312] (AB200346)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-SGSH/HSS antibody [EPR17312] (ab200346) at 1/10000 dilution

All lanes:

Human prostate cancer lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 57 kDa

Observed band size: 57 kDa

false

Exposure time: 30s

Supplier Data

Western blot - Anti-SGSH/HSS antibody [EPR17312] (AB200346)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-SGSH/HSS antibody [EPR17312] (ab200346) at 1/10000 dilution

All lanes:

MCF7 (Human breast adenocarcinoma cell line) whole cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 57 kDa

Observed band size: 57 kDa

false

Exposure time: 3min

Supplier Data

Western blot - Anti-SGSH/HSS antibody [EPR17312] (AB200346)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-SGSH/HSS antibody [EPR17312] (ab200346) at 1/1000 dilution

All lanes:

HepG2 (Human liver hepatocellular carcinoma) whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 57 kDa

Observed band size: 57 kDa

false

Exposure time: 3min

Carrier free

Anti-SGSH/HSS antibody [EPR17312] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR17312

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/250", "ICCIF-species-notes": "<p></p>" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The SGSH protein also known as N-sulfoglucosamine sulfohydrolase or HSS is a lysosomal enzyme with a molecular weight of approximately 56 kDa. It is primarily expressed in tissues with high turnover of glycosaminoglycans especially in the liver and brain. This enzyme localizes inside the lysosomes and plays a significant role in the degradation process of heparan sulfate. SGSH expression is important for maintaining normal cell function particularly in tissues that require constant renewal of extracellular matrix components.

Biological function summary

The enzyme acts to cleave sulfate groups from N-sulfated glucosamine residues in heparan sulfate being an important component of the degradation pathway of glycosaminoglycan. SGSH works within a lysosomal degradation complex alongside other enzymes such as iduronate-2-sulfatase and heparan-alpha-glucosaminide N-acetyltransferase each responsible for different steps in the breakdown of complex carbohydrates. Together they facilitate the complete degradation of heparan sulfate into its basic monosaccharide components.

Pathways

SGSH is integral to lysosomal degradation pathways functioning alongside proteins like alpha-L-iduronidase in the catabolic process of heparan sulfate. This breakdown pathway is essential for recycling cellular components and maintaining cellular homeostasis. The efficiencies and deficiencies in these pathways influence cellular functionality and have regulatory roles in broader metabolic circuits.

Mutations in the SGSH gene lead to Sanfilippo syndrome type IIIA also known as mucopolysaccharidosis type IIIA. This genetic disorder impairs the degradation of heparan sulfate causing an accumulation of glycosaminoglycans in cells leading to neurodegenerative conditions. In Sanfilippo syndrome type IIIA the SGSH deficiency has connections with related lysosomal storage disorders involving proteins like alpha-N-acetylglucosaminidase which further illustrate the importance of coordinated lysosomal activities.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes a step in lysosomal heparan sulfate degradation.

See full target information SGSH

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

The Journal of biological chemistry 298:102625 PubMed36306823

2022

Intracerebroventricular dosing of N-sulfoglucosamine sulfohydrolase in mucopolysaccharidosis IIIA mice reduces markers of brain lysosomal dysfunction.

Applications

Unspecified application

Species

Unspecified reactive species

Jenna Magat,Samantha Jones,Brian Baridon,Vishal Agrawal,Hio Wong,Alexander Giaramita,Linley Mangini,Britta Handyside,Catherine Vitelli,Monica Parker,Natasha Yeung,Yu Zhou,Erno Pungor,Ilya Slabodkin,Olivia Gorostiza,Allora Aguilera,Melanie J Lo,Saida Alcozie,Terri M Christianson,Pascale M N Tiger,Jon Vincelette,Sylvia Fong,Geuncheol Gil,Chuck Hague,Roger Lawrence,Daniel J Wendt,Jonathan H Lebowitz,Stuart Bunting,Sherry Bullens,Brett E Crawford,Sushmita M Roy,Josh C Woloszynek

Annals of neurology 82:686-696 PubMed29023963

2017

Prediction of phenotypic severity in mucopolysaccharidosis type IIIA.

Applications

ICC/IF, WB

Species

Human, Human

Suzan J G Knottnerus,Stephanie C M Nijmeijer,Lodewijk IJlst,Heleen Te Brinke,Naomi van Vlies,Frits A Wijburg

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SGSH/HSS antibody [EPR17312]

Immunocytochemistry/ Immunofluorescence - Anti-SGSH/HSS antibody [EPR17312] (AB200346)

Immunocytochemistry/ Immunofluorescence - Anti-SGSH/HSS antibody [EPR17312] (AB200346)

Western blot - Anti-SGSH/HSS antibody [EPR17312] (AB200346)

Western blot - Anti-SGSH/HSS antibody [EPR17312] (AB200346)

Western blot - Anti-SGSH/HSS antibody [EPR17312] (AB200346)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise