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AB249662

Anti-SGSM1/RUTBC2 antibody [EPR11563] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal SGSM1/RUTBC2 antibody. Carrier free. Suitable for ICC/IF, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

View Alternative Names

KIAA1941, RUTBC2, SGSM1, Small G protein signaling modulator 1, RUN and TBC1 domain-containing protein 2

3 Images
Flow Cytometry (Intracellular) - Anti-SGSM1/RUTBC2 antibody [EPR11563] - BSA and Azide free (AB249662)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-SGSM1/RUTBC2 antibody [EPR11563] - BSA and Azide free (AB249662)

This data was developed using ab171943, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of permeabilized 293T cellslabelling SGSM1/RUTBC2 with ab171943 at 1/10 (red) or a rabbit IgG (negative) (green).

Immunocytochemistry/ Immunofluorescence - Anti-SGSM1/RUTBC2 antibody [EPR11563] - BSA and Azide free (AB249662)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SGSM1/RUTBC2 antibody [EPR11563] - BSA and Azide free (AB249662)

This data was developed using ab171943, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 293T cells labeling SGSM1/RUTBC2 with ab171943 at 1/100 (red). DAPI nuclear staining (blue).

Western blot - Anti-SGSM1/RUTBC2 antibody [EPR11563] - BSA and Azide free (AB249662)
  • WB

Supplier Data

Western blot - Anti-SGSM1/RUTBC2 antibody [EPR11563] - BSA and Azide free (AB249662)

This data was developed using ab171943, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-SGSM1/RUTBC2 antibody [EPR11563] (<a href='/en-us/products/primary-antibodies/sgsm1-rutbc2-antibody-epr11563-ab171943'>ab171943</a>) at 1/1000 dilution

Lane 1:

Human fetal brain tissue lysate at 10 µg

Lane 2:

Human fetal kidney tissue lysate at 10 µg

Lane 3:

Human fetal heart tissue lysate at 10 µg

Lane 4:

U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) cell lysate at 10 µg

Lane 5:

293T (Human embryonic kidney epithelial cell) cell lysate at 10 µg

Predicted band size: 129 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR11563

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

Flow Cyt (Intra), ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "IHC" : {"fullname" : "Immunohistochemistry", "shortname":"IHC"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "IHC-species-checked": "notRecommended", "IHC-species-dilution-info": "", "IHC-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "IHC-species-checked": "notRecommended", "IHC-species-dilution-info": "", "IHC-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "IHC-species-checked": "notRecommended", "IHC-species-dilution-info": "", "IHC-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

ab249662 is the carrier-free version of ab171943.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SGSM1 also known as RUTBC2 serves as a Rab GTPase-activating protein. It has a mass of approximately 109 kDa. This protein interacts with small GTPases of the Rab family which are pivotal in intracellular vesicle trafficking. SGSM1/RUTBC2 catalyzes the hydrolysis of GTP to GDP on Rab proteins. This activity impacts the regulation of vesicular transport processes. SGSM1/RUTBC2 expression is seen in various tissues indicating its functional diversity.
Biological function summary

SGSM1/RUTBC2 modulates vesicular trafficking and membrane dynamics. It is not part of a large complex but acts as a solitary enzyme influencing membrane trafficking by its interaction with Rabs. Specifically it regulates endocytic and exocytic pathways. Rabs act as molecular switches cycling between active GTP-bound and inactive GDP-bound states and SGSM1 helps facilitate this conversion impacting cellular transport and membrane dynamics.

Pathways

SGSM1/RUTBC2 plays a significant role in endocytic recycling and autophagy pathways. It interacts with Rab7 an important player in the late endocytic pathway and Rab32 integral to lysosomal function. Through mediating the activity of these Rabs SGSM1 influences the trafficking and recycling of membrane proteins and can affect a generation of autophagosomes. This regulation ensures correct cargo delivery and recycling maintaining cellular homeostasis.

SGSM1/RUTBC2 has been associated with neurodegenerative conditions and cancer. Alterations in its expression or function could contribute to improper vesicular transport impacting cellular health and homeostasis. In Alzheimer’s disease dysfunctions of Rab activity influenced by SGSM1 have been noted. Additionally its link to Rab7 suggests a potential involvement in cancer progression as Rab7 dysregulation can affect tumor cell migration and metastasis. Understanding SGSM1 interactions provides insights into these pathologies potentially offering therapeutic targets.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Interacts with numerous Rab family members, functioning as Rab effector for some, and as GTPase activator for others. Promotes GTP hydrolysis by RAB34 and RAB36. Probably functions as a GTPase effector with RAB9A and RAB9B; does not stimulate GTP hydrolysis with RAB9A and RAB9B.
See full target information SGSM1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Oxidative medicine and cellular longevity 2021:8379962 PubMed34630853

2021

circ_0023461 Silencing Protects Cardiomyocytes from Hypoxia-Induced Dysfunction through Targeting miR-370-3p/PDE4D Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Kai Ren,Buying Li,Liqing Jiang,Zhiheng Liu,Fan Wu,Yi Zhang,Jincheng Liu,Weixun Duan
View all publications
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Product promise

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