AB246881

Anti-SGT1/ECD antibody

Name: Anti-SGT1/ECD antibody (ab246881) | Abcam
Brand: Abcam Limited
SKU: AB246881

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(1 Publication)

Rabbit Polyclonal SGT1/ECD antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human ECD aa 500 to C-terminus.

View Alternative Names

Protein ecdysoneless homolog, Human suppressor of GCR two, hSGT1, ECD

6 Images

Immunocytochemistry/ Immunofluorescence - Anti-SGT1/ECD antibody (AB246881)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-SGT1/ECD antibody (AB246881)

PFA-fixed, Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells stained for SGT1/ECD (green) using ab246881 at 4 μg/ml in ICC/IF.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SGT1/ECD antibody (AB246881)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SGT1/ECD antibody (AB246881)

Paraffin-embedded human duodenum tissue stained for SGT1/ECD using ab246881 at 1/200 dilution in immunohistochemical analysis.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SGT1/ECD antibody (AB246881)

Paraffin-embedded human colon tissue stained for SGT1/ECD using ab246881 at 1/200 dilution in immunohistochemical analysis.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SGT1/ECD antibody (AB246881)

Paraffin-embedded human endometrium tissue stained for SGT1/ECD using ab246881 at 1/200 dilution in immunohistochemical analysis.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SGT1/ECD antibody (AB246881)

Paraffin-embedded human testis tissue stained for SGT1/ECD using ab246881 at 1/200 dilution in immunohistochemical analysis.

Unknown

Western blot - Anti-SGT1/ECD antibody (AB246881)

All lanes:

Western blot - Anti-SGT1/ECD antibody (ab246881) at 0.4 µg/mL

Lane 1:

RT4 (human urinary bladder cancer cell line) cell lysate

Lane 2:

U-251 MG (human brain glioma cell line) cell lysate

Predicted band size: 73 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human ECD aa 500 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

O95905

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "Fixation/Permeabilization: PFA/Triton X-100." } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SGT1/ECD also known as SGT1 (Suppressor of G2 allele of SKP1) or ECD (Ectodermal Dysplasia protein) is a regulatory protein with a mass of approximately 36 kDa. It is highly expressed in various eukaryotic cells and tissues. This protein plays an important mechanical role by facilitating the assembly and stabilization of protein complexes. SGT1/ECD acts as a co-chaperone assisting HSP90 in its function which is critical for client protein folding and activity.

Biological function summary

SGT1/ECD participates in the assembly of multi-protein complexes. It is also intertwined with the SCF (SKP1-Cullin1-F-box) ubiquitin ligase complex where it contributes to substrate recognition and proteasomal degradation. Furthermore SGT1/ECD is important in regulating the cell cycle and signal transduction processes involving plant immunity and kinetochore function during chromosome segregation.

Pathways

SGT1/ECD engages in both the plant and animal kingdoms. In plants it plays a role in the resistance (R) gene-mediated signaling pathways and the jasmonic acid pathway where it pairs with chaperones like HSP90. In animals it links with cell cycle pathways affecting proteins such as SKP1 and HSP90. Through these interconnected pathways SGT1/ECD is linked to vital signaling mechanisms impacting cellular responses and stress tolerance.

SGT1/ECD shows connections to cancer and immune response dysregulation. Within cancer biology altered SGT1/ECD expression influences the stability of oncogenic client proteins via HSP90. Additionally in the context of immune disorders it is involved in plant immunity defects where malfunction in SGT1/ECD-mediated pathways can lead to impaired disease resistance. Its associations with proteins like HSP90 and components of the SCF complex highlight its relevance in maintaining cellular homeostasis and proper immune functions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Regulator of p53/TP53 stability and function. Inhibits MDM2-mediated degradation of p53/TP53 possibly by cooperating in part with TXNIP (PubMed : 16849563, PubMed : 23880345). May be involved transcriptional regulation. In vitro has intrinsic transactivation activity enhanced by EP300. May be a transcriptional activator required for the expression of glycolytic genes (PubMed : 19919181, PubMed : 9928932). Involved in regulation of cell cycle progression. Proposed to disrupt Rb-E2F binding leading to transcriptional activation of E2F proteins (PubMed : 19640839). The cell cycle -regulating function may depend on its RUVBL1-mediated association with the R2TP complex (PubMed : 26711270). May play a role in regulation of pre-mRNA splicing (PubMed : 24722212).

See full target information ECD

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

BMC cancer 23:586 PubMed37365518

2023

Expression profiles and functional prediction of histone acetyltransferases of the MYST family in kidney renal clear cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Fan Liang,Xiangke Li,Xiaoman Shen,Runlei Yang,Chuan Chen

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SGT1/ECD antibody

Immunocytochemistry/ Immunofluorescence - Anti-SGT1/ECD antibody (AB246881)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SGT1/ECD antibody (AB246881)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SGT1/ECD antibody (AB246881)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SGT1/ECD antibody (AB246881)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SGT1/ECD antibody (AB246881)

Western blot - Anti-SGT1/ECD antibody (AB246881)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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