Rabbit Polyclonal SGT1/ECD antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human ECD aa 500 to C-terminus.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human ECD aa 500 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link O95905
Reactivity data
Select an application| IHC-P | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/200.00000 - 1/500.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.04000-0.40000 µg/mL | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.25000-2.00000 µg/mL | Notes Fixation/Permeabilization: PFA/Triton X-100. |
Target data
Function
Regulator of p53/TP53 stability and function. Inhibits MDM2-mediated degradation of p53/TP53 possibly by cooperating in part with TXNIP (PubMed:16849563, PubMed:23880345). May be involved transcriptional regulation. In vitro has intrinsic transactivation activity enhanced by EP300. May be a transcriptional activator required for the expression of glycolytic genes (PubMed:19919181, PubMed:9928932). Involved in regulation of cell cycle progression. Proposed to disrupt Rb-E2F binding leading to transcriptional activation of E2F proteins (PubMed:19640839). The cell cycle -regulating function may depend on its RUVBL1-mediated association with the R2TP complex (PubMed:26711270). May play a role in regulation of pre-mRNA splicing (PubMed:24722212).
Alternative names
Protein ecdysoneless homolog, Human suppressor of GCR two, hSGT1, ECD
Recommended products
Rabbit Polyclonal SGT1/ECD antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human ECD aa 500 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human ECD aa 500 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link O95905
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
SGT1/ECD also known as SGT1 (Suppressor of G2 allele of SKP1) or ECD (Ectodermal Dysplasia protein) is a regulatory protein with a mass of approximately 36 kDa. It is highly expressed in various eukaryotic cells and tissues. This protein plays an important mechanical role by facilitating the assembly and stabilization of protein complexes. SGT1/ECD acts as a co-chaperone assisting HSP90 in its function which is critical for client protein folding and activity.
- Biological function summary
SGT1/ECD participates in the assembly of multi-protein complexes. It is also intertwined with the SCF (SKP1-Cullin1-F-box) ubiquitin ligase complex where it contributes to substrate recognition and proteasomal degradation. Furthermore SGT1/ECD is important in regulating the cell cycle and signal transduction processes involving plant immunity and kinetochore function during chromosome segregation.
- Pathways
SGT1/ECD engages in both the plant and animal kingdoms. In plants it plays a role in the resistance (R) gene-mediated signaling pathways and the jasmonic acid pathway where it pairs with chaperones like HSP90. In animals it links with cell cycle pathways affecting proteins such as SKP1 and HSP90. Through these interconnected pathways SGT1/ECD is linked to vital signaling mechanisms impacting cellular responses and stress tolerance.
- Associated diseases and disorders
SGT1/ECD shows connections to cancer and immune response dysregulation. Within cancer biology altered SGT1/ECD expression influences the stability of oncogenic client proteins via HSP90. Additionally in the context of immune disorders it is involved in plant immunity defects where malfunction in SGT1/ECD-mediated pathways can lead to impaired disease resistance. Its associations with proteins like HSP90 and components of the SCF complex highlight its relevance in maintaining cellular homeostasis and proper immune functions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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6 product images
Western blot - Anti-SGT1/ECD antibody (ab246881)
All lanes: Western blot - Anti-SGT1/ECD antibody (ab246881) at 0.4 µg/mL
Lane 1: RT4 (human urinary bladder cancer cell line) cell lysate
Lane 2: U-251 MG (human brain glioma cell line) cell lysate
Predicted band size: 73 kDa
Immunocytochemistry/ Immunofluorescence - Anti-SGT1/ECD antibody (ab246881)
PFA-fixed, Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells stained for SGT1/ECD (green) using ab246881 at 4 μg/ml in ICC/IF.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SGT1/ECD antibody (ab246881)
Paraffin-embedded human duodenum tissue stained for SGT1/ECD using ab246881 at 1/200 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SGT1/ECD antibody (ab246881)
Paraffin-embedded human testis tissue stained for SGT1/ECD using ab246881 at 1/200 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SGT1/ECD antibody (ab246881)
Paraffin-embedded human endometrium tissue stained for SGT1/ECD using ab246881 at 1/200 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SGT1/ECD antibody (ab246881)
Paraffin-embedded human colon tissue stained for SGT1/ECD using ab246881 at 1/200 dilution in immunohistochemical analysis.
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