AB99293

Anti-SGT1/ECD antibody

Name: Anti-SGT1/ECD antibody (ab99293) | Abcam
Brand: Abcam Limited
SKU: AB99293
Price: 535 USD
Availability: InStock
Rating: 4 (1 reviews)

(1 Review)

(2 Publications)

Rabbit Polyclonal SGT1/ECD antibody. Suitable for IP, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human ECD aa 450-500.

View Alternative Names

Protein ecdysoneless homolog, Human suppressor of GCR two, hSGT1, ECD

2 Images

Unknown

Immunoprecipitation - Anti-SGT1/ECD antibody (AB99293)

Lane 1 : Detection of Human SGT1/ECD by Immunoprecipitation from Hela ( 1 mg for IP, 20% of IP loaded), using 3μg/mg lysate. Lane 2 : Control IgG

All lanes:

Immunoprecipitation - Anti-SGT1/ECD antibody (ab99293)

Predicted band size: 73 kDa

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Unknown

Western blot - Anti-SGT1/ECD antibody (AB99293)

All lanes:

Western blot - Anti-SGT1/ECD antibody (ab99293) at 0.04 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Lane 4:

293T whole cell lysate at 50 µg

Predicted band size: 73 kDa

false

Exposure time: 3s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human ECD aa 450-500. The exact immunogen used to generate this antibody is proprietary information.

O95905

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Elephant": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Goat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Guinea pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SGT1/ECD also known as SGT1 (Suppressor of G2 allele of SKP1) or ECD (Ectodermal Dysplasia protein) is a regulatory protein with a mass of approximately 36 kDa. It is highly expressed in various eukaryotic cells and tissues. This protein plays an important mechanical role by facilitating the assembly and stabilization of protein complexes. SGT1/ECD acts as a co-chaperone assisting HSP90 in its function which is critical for client protein folding and activity.

Biological function summary

SGT1/ECD participates in the assembly of multi-protein complexes. It is also intertwined with the SCF (SKP1-Cullin1-F-box) ubiquitin ligase complex where it contributes to substrate recognition and proteasomal degradation. Furthermore SGT1/ECD is important in regulating the cell cycle and signal transduction processes involving plant immunity and kinetochore function during chromosome segregation.

Pathways

SGT1/ECD engages in both the plant and animal kingdoms. In plants it plays a role in the resistance (R) gene-mediated signaling pathways and the jasmonic acid pathway where it pairs with chaperones like HSP90. In animals it links with cell cycle pathways affecting proteins such as SKP1 and HSP90. Through these interconnected pathways SGT1/ECD is linked to vital signaling mechanisms impacting cellular responses and stress tolerance.

SGT1/ECD shows connections to cancer and immune response dysregulation. Within cancer biology altered SGT1/ECD expression influences the stability of oncogenic client proteins via HSP90. Additionally in the context of immune disorders it is involved in plant immunity defects where malfunction in SGT1/ECD-mediated pathways can lead to impaired disease resistance. Its associations with proteins like HSP90 and components of the SCF complex highlight its relevance in maintaining cellular homeostasis and proper immune functions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Regulator of p53/TP53 stability and function. Inhibits MDM2-mediated degradation of p53/TP53 possibly by cooperating in part with TXNIP (PubMed : 16849563, PubMed : 23880345). May be involved transcriptional regulation. In vitro has intrinsic transactivation activity enhanced by EP300. May be a transcriptional activator required for the expression of glycolytic genes (PubMed : 19919181, PubMed : 9928932). Involved in regulation of cell cycle progression. Proposed to disrupt Rb-E2F binding leading to transcriptional activation of E2F proteins (PubMed : 19640839). The cell cycle -regulating function may depend on its RUVBL1-mediated association with the R2TP complex (PubMed : 26711270). May play a role in regulation of pre-mRNA splicing (PubMed : 24722212). Participates together with DDX39A in mRNA nuclear export (PubMed : 33941617).

See full target information ECD

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

iScience 27:109974 PubMed38832028

2024

Deficiency in Prader-Willi syndrome gene leads to attenuated cardiac contractility.

Applications

Unspecified application

Species

Unspecified reactive species

Yufan Dong,Renbin Lu,Hui Cao,Jing Zhang,Xiushan Wu,Yun Deng,Jia-Da Li

The Journal of cell biology 210:705-16 PubMed26323688

2015

Degradation of HK2 by chaperone-mediated autophagy promotes metabolic catastrophe and cell death.

Applications

Unspecified application

Species

Unspecified reactive species

Hong-Guang Xia,Ayaz Najafov,Jiefei Geng,Lorena Galan-Acosta,Xuemei Han,Yuan Guo,Bing Shan,Yaoyang Zhang,Erik Norberg,Tao Zhang,Lifeng Pan,Junli Liu,Jonathan L Coloff,Dimitry Ofengeim,Hong Zhu,Kejia Wu,Yu Cai,John R Yates,Zhengjiang Zhu,Junying Yuan,Helin Vakifahmetoglu-Norberg

View all publications

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-SGT1/ECD antibody

Immunoprecipitation - Anti-SGT1/ECD antibody (AB99293)

Western blot - Anti-SGT1/ECD antibody (AB99293)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise