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Rat Recombinant Monoclonal SH2D1A/SAP antibody. Carrier free. Suitable for WB and reacts with Human samples.

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Images

Western blot - Anti-SH2D1A/SAP antibody [1D12] - BSA and Azide free (AB255862), expandable thumbnail

Key facts

Isotype
IgG2a
Host species
Rat
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow CytWB
Human
Not recommended
Tested
Mouse
Not recommended
Not recommended
Rat
Not recommended
Not recommended

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Target data

Function

Cytoplasmic adapter regulating receptors of the signaling lymphocytic activation molecule (SLAM) family such as SLAMF1, CD244, LY9, CD84, SLAMF6 and SLAMF7. In SLAM signaling seems to cooperate with SH2D1B/EAT-2. Initially it has been proposed that association with SLAMF1 prevents SLAMF1 binding to inhibitory effectors including INPP5D/SHIP1 and PTPN11/SHP-2 (PubMed:11806999). However, by simultaneous interactions, recruits FYN which subsequently phosphorylates and activates SLAMF1 (PubMed:12458214). Positively regulates CD244/2B4- and CD84-mediated natural killer (NK) cell functions. Can also promote CD48-, SLAMF6 -, LY9-, and SLAMF7-mediated NK cell activation. In the context of NK cell-mediated cytotoxicity enhances conjugate formation with target cells (By similarity). May also regulate the activity of the neurotrophin receptors NTRK1, NTRK2 and NTRK3.

Alternative names

DSHP, SAP, SH2D1A, SH2 domain-containing protein 1A, Duncan disease SH2-protein, Signaling lymphocytic activation molecule-associated protein, T-cell signal transduction molecule SAP, SLAM-associated protein

Recommended products

Rat Recombinant Monoclonal SH2D1A/SAP antibody. Carrier free. Suitable for WB and reacts with Human samples.

Key facts

Isotype
IgG2a
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
1D12
Purification technique
Affinity purification Protein A
Light chain type
kappa
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab255862 is the carrier-free version of ab255862.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

SH2D1A also known as SLAM-associated protein (SAP) is a small intracellular protein with a molecular weight of approximately 15 kDa. It is primarily expressed in T cells and natural killer (NK) cells playing important role in immune signaling. SAP contains SH2 domains that facilitate its interaction with signaling lymphocytic activation molecule (SLAM) family receptors. These interactions mediate downstream signaling cascades that control immune responses.

Biological function summary

The SAP protein influences T cell and NK cell activity by modifying signaling pathways involved in cell development and function. SAP does not function alone; it is part of a signaling complex with SLAM and other adaptor molecules. This complex regulates cytokine production and cytotoxic activity which are important for maintaining effective immune responses against infections.

Pathways

SH2D1A is an important component in the SLAM signaling pathway which interacts closely with the ITK signaling pathway. These pathways are essential for proper development and function of T cells and NK cells. SAP also modulates interactions with proteins such as Fyn a tyrosine kinase linked to T cell receptor signaling. Through these pathways it influences cellular processes that ensure effective immune surveillance and response.

Associated diseases and disorders

SH2D1A mutations or dysfunction are directly connected to X-linked lymphoproliferative disease (XLP1) a condition characterized by the inability to regulate immune responses often resulting in severe and life-threatening infections. The protein is associated with immune dysregulation observed in the disease contributing to defective signaling in T cells and NK cells. Other disorders related to SH2D1A dysfunction include certain lymphomas where aberrant expression of this and related proteins such as SLAM receptors affect cellular proliferation and immune surveillance.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • Western blot - Anti-SH2D1A/SAP antibody [1D12] - BSA and Azide free (ab255862), expandable thumbnail

    Western blot - Anti-SH2D1A/SAP antibody [1D12] - BSA and Azide free (ab255862)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    Negative control: Raji (PMID:11313386; PMID:11054674).

    Exposure time: lane 1, 2:10 seconds lane 3, 4: 37 seconds

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-SH2D1A/SAP antibody [1D12] ab252866).

    All lanes: Western blot - Anti-SH2D1A/SAP antibody [1D12] (Anti-SH2D1A/SAP antibody [1D12] ab252866) at 1/1000 dilution

    Lane 1: MOLT-4 (human lymphoblastic leukemia T lymphoblast), whole cell lysate at 20 µg

    Lane 2: Jurkat (human T cell leukemia T lymphocyte), whole cell lysate at 20 µg

    Lane 3: Raji (human Burkitt's lymphoma B lymphocyte), whole cell lysate at 20 µg

    Lane 4: HDLM-2 (human Hodgkin lymphoma), whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rat IgG H&L (HRP) (Goat Anti-Rat IgG H&L (HRP) ab205720) at 1/5000 dilution

    Predicted band size: 14 kDa

    Observed band size: 14 kDa, 40 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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