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AB255862

Anti-SH2D1A/SAP antibody [1D12] - BSA and Azide free

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Rat Recombinant Monoclonal SH2D1A/SAP antibody. Carrier free. Suitable for WB and reacts with Human samples.

View Alternative Names

DSHP, SAP, SH2D1A, SH2 domain-containing protein 1A, Duncan disease SH2-protein, Signaling lymphocytic activation molecule-associated protein, T-cell signal transduction molecule SAP, SLAM-associated protein

1 Images
Western blot - Anti-SH2D1A/SAP antibody [1D12] - BSA and Azide free (AB255862)
  • WB

Lab

Western blot - Anti-SH2D1A/SAP antibody [1D12] - BSA and Azide free (AB255862)

Blocking and diluting buffer and concentration : 5% NFDM/TBST

Negative control : Raji (PMID : 11313386; PMID : 11054674).

Exposure time : lane 1, 2 : 10 seconds lane 3, 4 : 37 seconds

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252866).

All lanes:

Western blot - Anti-SH2D1A/SAP antibody [1D12] (<a href='/en-us/products/primary-antibodies/sh2d1a-sap-antibody-1d12-ab252866'>ab252866</a>) at 1/1000 dilution

Lane 1:

MOLT-4 (human lymphoblastic leukemia T lymphoblast), whole cell lysate at 20 µg

Lane 2:

Jurkat (human T cell leukemia T lymphocyte), whole cell lysate at 20 µg

Lane 3:

Raji (human Burkitt's lymphoma B lymphocyte), whole cell lysate at 20 µg

Lane 4:

HDLM-2 (human Hodgkin lymphoma), whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rat IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rat-igg-h-l-hrp-ab205720'>ab205720</a>) at 1/5000 dilution

Predicted band size: 14 kDa

Observed band size: 14 kDa,40 kDa

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Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

1D12

Isotype

IgG2a

Light chain type

kappa

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" }, "Mouse": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" }, "Rat": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" } } }
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Product details

ab255862 is the carrier-free version of ab255862.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SH2D1A also known as SLAM-associated protein (SAP) is a small intracellular protein with a molecular weight of approximately 15 kDa. It is primarily expressed in T cells and natural killer (NK) cells playing important role in immune signaling. SAP contains SH2 domains that facilitate its interaction with signaling lymphocytic activation molecule (SLAM) family receptors. These interactions mediate downstream signaling cascades that control immune responses.
Biological function summary

The SAP protein influences T cell and NK cell activity by modifying signaling pathways involved in cell development and function. SAP does not function alone; it is part of a signaling complex with SLAM and other adaptor molecules. This complex regulates cytokine production and cytotoxic activity which are important for maintaining effective immune responses against infections.

Pathways

SH2D1A is an important component in the SLAM signaling pathway which interacts closely with the ITK signaling pathway. These pathways are essential for proper development and function of T cells and NK cells. SAP also modulates interactions with proteins such as Fyn a tyrosine kinase linked to T cell receptor signaling. Through these pathways it influences cellular processes that ensure effective immune surveillance and response.

SH2D1A mutations or dysfunction are directly connected to X-linked lymphoproliferative disease (XLP1) a condition characterized by the inability to regulate immune responses often resulting in severe and life-threatening infections. The protein is associated with immune dysregulation observed in the disease contributing to defective signaling in T cells and NK cells. Other disorders related to SH2D1A dysfunction include certain lymphomas where aberrant expression of this and related proteins such as SLAM receptors affect cellular proliferation and immune surveillance.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Cytoplasmic adapter regulating receptors of the signaling lymphocytic activation molecule (SLAM) family such as SLAMF1, CD244, LY9, CD84, SLAMF6 and SLAMF7. In SLAM signaling seems to cooperate with SH2D1B/EAT-2. Initially it has been proposed that association with SLAMF1 prevents SLAMF1 binding to inhibitory effectors including INPP5D/SHIP1 and PTPN11/SHP-2 (PubMed : 11806999). However, by simultaneous interactions, recruits FYN which subsequently phosphorylates and activates SLAMF1 (PubMed : 12458214). Positively regulates CD244/2B4- and CD84-mediated natural killer (NK) cell functions. Can also promote CD48-, SLAMF6 -, LY9-, and SLAMF7-mediated NK cell activation. In the context of NK cell-mediated cytotoxicity enhances conjugate formation with target cells (By similarity). May also regulate the activity of the neurotrophin receptors NTRK1, NTRK2 and NTRK3.
See full target information SH2D1A
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com