Rabbit Polyclonal SH2D1A/SAP antibody. Suitable for WB, ICC/IF and reacts with Human, Mouse samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human SH2D1A.
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- Signal transduction and targeted therapy 7:602022Single-cell transcriptomic profiling unravels the adenoma-initiation role of protein tyrosine kinases during colorectal tumorigenesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaobo Zheng,Jinen Song,Chune Yu,Zongguang Zhou,Xiaowei Liu,Jing Yu,Guangchao Xu,Jiqiao Yang,Xiujing He,Xin Bai,Ya Luo,Yu Bao,Huifang Li,Lie Yang,Mingqing Xu,Nan Song,Xiaodong Su,Jie Xu,Xuelei Ma,Hubing ShiPubMed 35221332
- Frontiers in physiology 11:5892020Regulatory Effect of Sishen Pill on Tfh Cells in Mice With Experimental Colitis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXue-Ke Liu et. al.PubMed 32581849
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Full Length Protein corresponding to Human SH2D1A. Database link O60880
Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Human | Tested | Expected |
| Mouse | Tested | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse | Dilution info - | Notes - |
ICC/IF
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Cytoplasmic adapter regulating receptors of the signaling lymphocytic activation molecule (SLAM) family such as SLAMF1, CD244, LY9, CD84, SLAMF6 and SLAMF7. In SLAM signaling seems to cooperate with SH2D1B/EAT-2. Initially it has been proposed that association with SLAMF1 prevents SLAMF1 binding to inhibitory effectors including INPP5D/SHIP1 and PTPN11/SHP-2 (PubMed:11806999). However, by simultaneous interactions, recruits FYN which subsequently phosphorylates and activates SLAMF1 (PubMed:12458214). Positively regulates CD244/2B4- and CD84-mediated natural killer (NK) cell functions. Can also promote CD48-, SLAMF6 -, LY9-, and SLAMF7-mediated NK cell activation. In the context of NK cell-mediated cytotoxicity enhances conjugate formation with target cells (By similarity). May also regulate the activity of the neurotrophin receptors NTRK1, NTRK2 and NTRK3.
Alternative names
DSHP, SAP, SH2D1A, SH2 domain-containing protein 1A, Duncan disease SH2-protein, Signaling lymphocytic activation molecule-associated protein, T-cell signal transduction molecule SAP, SLAM-associated protein
Recommended products
Rabbit Polyclonal SH2D1A/SAP antibody. Suitable for WB, ICC/IF and reacts with Human, Mouse samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human SH2D1A.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Full Length Protein corresponding to Human SH2D1A. Database link O60880
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
SH2D1A also known as SLAM-associated protein (SAP) is a small intracellular protein with a molecular weight of approximately 15 kDa. It is primarily expressed in T cells and natural killer (NK) cells playing important role in immune signaling. SAP contains SH2 domains that facilitate its interaction with signaling lymphocytic activation molecule (SLAM) family receptors. These interactions mediate downstream signaling cascades that control immune responses.
- Biological function summary
The SAP protein influences T cell and NK cell activity by modifying signaling pathways involved in cell development and function. SAP does not function alone; it is part of a signaling complex with SLAM and other adaptor molecules. This complex regulates cytokine production and cytotoxic activity which are important for maintaining effective immune responses against infections.
- Pathways
SH2D1A is an important component in the SLAM signaling pathway which interacts closely with the ITK signaling pathway. These pathways are essential for proper development and function of T cells and NK cells. SAP also modulates interactions with proteins such as Fyn a tyrosine kinase linked to T cell receptor signaling. Through these pathways it influences cellular processes that ensure effective immune surveillance and response.
- Associated diseases and disorders
SH2D1A mutations or dysfunction are directly connected to X-linked lymphoproliferative disease (XLP1) a condition characterized by the inability to regulate immune responses often resulting in severe and life-threatening infections. The protein is associated with immune dysregulation observed in the disease contributing to defective signaling in T cells and NK cells. Other disorders related to SH2D1A dysfunction include certain lymphomas where aberrant expression of this and related proteins such as SLAM receptors affect cellular proliferation and immune surveillance.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Western blot - Anti-SH2D1A/SAP antibody (ab185810)
Blocking buffer: 3% nonfat dry milk in TBST.
All lanes: Western blot - Anti-SH2D1A/SAP antibody (ab185810) at 1/1000 dilution
Lane 1: Human Jurkat at 25 µg
Lane 2: Human PC-3 at 25 µg
Lane 3: Mouse spleen at 25 µg
SecondaryAll lanes: HRP Goat Anti-Rabbit IgG (H+L) at 1/100000 dilution
Predicted band size: 14 kDa
Observed band size: 17 kDa
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