AB109120

Anti-SH2D1A/SAP antibody [EPR3168]

RabMAb
Recombinant
What is this?

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(2 Publications)

Rabbit Recombinant Monoclonal SH2D1A/SAP antibody. Suitable for WB and reacts with Rat, Human, Mouse samples. Cited in 2 publications.

View Alternative Names

DSHP, SAP, SH2D1A, SH2 domain-containing protein 1A, Duncan disease SH2-protein, Signaling lymphocytic activation molecule-associated protein, T-cell signal transduction molecule SAP, SLAM-associated protein

3 Images

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Western blot - Anti-SH2D1A/SAP antibody [EPR3168] (AB109120)

All lanes:

Western blot - Anti-SH2D1A/SAP antibody [EPR3168] (ab109120) at 1/1000 dilution

Lane 1:

MOLT-4 cell line lysate at 10 µg

Lane 2:

Jurkat cell line lysate at 10 µg

Lane 3:

K562 cell line lysate at 10 µg

Predicted band size: 14 kDa

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Lab

Western blot - Anti-SH2D1A/SAP antibody [EPR3168] (AB109120)

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-SH2D1A/SAP antibody [EPR3168] (ab109120) at 1/2000 dilution

Lane 1:

MOLT-4 whole cell lysate at 10 µg

Lane 2:

Jurkat whole cell lysate at 10 µg

Lane 3:

K562 whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Predicted band size: 14 kDa

Observed band size: 16 kDa

false

Lab

Western blot - Anti-SH2D1A/SAP antibody [EPR3168] (AB109120)

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-SH2D1A/SAP antibody [EPR3168] (ab109120) at 1/2000 dilution

Lane 1:

PC-12 whole cell lysate at 10 µg

Lane 2:

Mouse brain tissue lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Predicted band size: 14 kDa

Observed band size: 16 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR3168

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "1/50 - 1/500", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "1/50 - 1/500", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "1/100 - 1/150", "ICCIF-species-notes": "" }, "Rat": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "1/50 - 1/500", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "1/100 - 1/150", "ICCIF-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SH2D1A also known as SLAM-associated protein (SAP) is a small intracellular protein with a molecular weight of approximately 15 kDa. It is primarily expressed in T cells and natural killer (NK) cells playing important role in immune signaling. SAP contains SH2 domains that facilitate its interaction with signaling lymphocytic activation molecule (SLAM) family receptors. These interactions mediate downstream signaling cascades that control immune responses.

Biological function summary

The SAP protein influences T cell and NK cell activity by modifying signaling pathways involved in cell development and function. SAP does not function alone; it is part of a signaling complex with SLAM and other adaptor molecules. This complex regulates cytokine production and cytotoxic activity which are important for maintaining effective immune responses against infections.

Pathways

SH2D1A is an important component in the SLAM signaling pathway which interacts closely with the ITK signaling pathway. These pathways are essential for proper development and function of T cells and NK cells. SAP also modulates interactions with proteins such as Fyn a tyrosine kinase linked to T cell receptor signaling. Through these pathways it influences cellular processes that ensure effective immune surveillance and response.

SH2D1A mutations or dysfunction are directly connected to X-linked lymphoproliferative disease (XLP1) a condition characterized by the inability to regulate immune responses often resulting in severe and life-threatening infections. The protein is associated with immune dysregulation observed in the disease contributing to defective signaling in T cells and NK cells. Other disorders related to SH2D1A dysfunction include certain lymphomas where aberrant expression of this and related proteins such as SLAM receptors affect cellular proliferation and immune surveillance.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Cytoplasmic adapter regulating receptors of the signaling lymphocytic activation molecule (SLAM) family such as SLAMF1, CD244, LY9, CD84, SLAMF6 and SLAMF7. In SLAM signaling seems to cooperate with SH2D1B/EAT-2. Initially it has been proposed that association with SLAMF1 prevents SLAMF1 binding to inhibitory effectors including INPP5D/SHIP1 and PTPN11/SHP-2 (PubMed : 11806999). However, by simultaneous interactions, recruits FYN which subsequently phosphorylates and activates SLAMF1 (PubMed : 12458214). Positively regulates CD244/2B4- and CD84-mediated natural killer (NK) cell functions. Can also promote CD48-, SLAMF6 -, LY9-, and SLAMF7-mediated NK cell activation. In the context of NK cell-mediated cytotoxicity enhances conjugate formation with target cells (By similarity). May also regulate the activity of the neurotrophin receptors NTRK1, NTRK2 and NTRK3.

See full target information SH2D1A

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cellular and molecular life sciences : CMLS 82:4 PubMed39680136

2024

Golgi pH elevation due to loss of V-ATPase subunit V0a2 function correlates with tissue-specific glycosylation changes and globozoospermia.

Applications

Unspecified application

Species

Unspecified reactive species

Johannes Kopp,Denise Jahn,Guido Vogt,Anthi Psoma,Edoardo Ratto,Willy Morelle,Nina Stelzer,Ingrid Hausser,Anne Hoffmann,Miguel Rodriguez de Los Santos,Leonard A Koch,Björn Fischer-Zirnsak,Christian Thiel,Wilhelm Palm,David Meierhofer,Geert van den Bogaart,François Foulquier,Andreas Meinhardt,Uwe Kornak

Medicine 101:e30951 PubMed36254040

2022

Potential pathogenic mechanism of type 1 X-linked lymphoproliferative syndrome caused by a mutation of SH2D1A gene in an infant: A case report.

Applications

Unspecified application

Species

Unspecified reactive species

Yanchun Wang,Yan Wang,Weimin Lu,Lvyan Tao,Yang Xiao,Yuantao Zhou,Xiaoli He,Yu Zhang,Li Li

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SH2D1A/SAP antibody [EPR3168]

Western blot - Anti-SH2D1A/SAP antibody [EPR3168] (AB109120)

Western blot - Anti-SH2D1A/SAP antibody [EPR3168] (AB109120)

Western blot - Anti-SH2D1A/SAP antibody [EPR3168] (AB109120)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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