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AB222814

Anti-SH2D1B antibody

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(1 Publication)

Rabbit Polyclonal SH2D1B antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human SH2D1B aa 1-100.

View Alternative Names

EAT2, SH2D1B, SH2 domain-containing protein 1B, EWS/FLI1-activated transcript 2, EAT-2

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SH2D1B antibody (AB222814)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SH2D1B antibody (AB222814)

Paraffin-embedded human colon cancer tissue stained for SH2D1B using ab222814 at 1/100 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-SH2D1B antibody (AB222814)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SH2D1B antibody (AB222814)

HepG2 (human liver hepatocellular carcinoma cell line) cells stained for SH2D1B (green) using ab222814 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488-congugated Goat Anti-Rabbit IgG (H+L) .

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human SH2D1B aa 1-100. The exact immunogen used to generate this antibody is proprietary information.

O14796

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purity >95%.
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SH2D1B also known as EAT-2 is an adaptor protein that has a significant role in immune cell signaling. It is about 15 kDa in size and contains a single SH2 domain which allows it to interact with phosphorylated tyrosine-containing proteins. SH2D1B is mainly expressed in natural killer (NK) cells and some T-cell populations. In these cells SH2D1B modulates signaling pathways by interacting with various receptors and other signaling proteins affecting cellular responses.
Biological function summary

SH2D1B influences immune cell activation and effector functions. It is part of a signaling complex that modulates the activity of NK cells and some T-cells influencing processes like cytotoxicity and cytokine production. By participating in this complex SH2D1B plays a role in regulating immune responses affecting conditions ranging from viral infections to cancer surveillance where adaptive immunity is modified.

Pathways

SH2D1B is involved in the regulation of the SLAM family receptor signaling pathway which is vital for the activation and function of NK cells. It interacts closely with other proteins in this pathway such as SAP (SLAM-associated protein) to influence signaling cascades that determine cell fate and activity. SH2D1B also participates in the NF-kB signaling pathway further affecting immune responses through modulation of gene expression involved in cell survival and differentiation.

SH2D1B has associations with immune system dysfunctions such as X-linked lymphoproliferative syndrome (XLP). This disorder involves unregulated immune responses and is linked to an overactive or misregulated SLAM receptor signaling where SH2D1B collaborates with SAP. Additionally variations in SH2D1B expression or function have potential links to autoimmune diseases due to its role in regulatory pathways of immune activation and suppression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Cytoplasmic adapter regulating receptors of the signaling lymphocytic activation molecule (SLAM) family such as CD84, SLAMF1, LY9 and CD244 (PubMed : 11689425). In SLAM signaling seems to cooperate with SH2D1A/SAP. Plays a role in regulation of effector functions of natural killer (NK) cells by controlling signal transduction through CD244/2B4 without effecting its tyrosine phosphorylation; downstream signaling involves PLCG1 and ERK activation (PubMed : 24687958). Activation of SLAMF7-mediated NK cell function does not effect receptor tyrosine phosphorylation but distal signaling (By similarity). In the context of NK cell-mediated cytotoxicity does not enhance conjugate formation with target cells but stimulates polarization of the microtubule-organizing center and cytotoxic granules toward the NK cell synapse (PubMed : 24687958). Negatively regulates CD40-induced cytokine production in dendritic cells downstream of SLAM family receptors probably by inducing activation of the PI3K pathway to inhibit p38 MAPK and JNK activation (By similarity).
See full target information SH2D1B
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 14:248 PubMed37024473

2023

Sphingomyelin 16:0 is a therapeutic target for neuronal death in acid sphingomyelinase deficiency.

Applications

Unspecified application

Species

Unspecified reactive species

Ángel Gaudioso,Xuntian Jiang,Josefina Casas,Edward H Schuchman,María Dolores Ledesma
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