Rabbit Polyclonal SH2D1B antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human SH2D1B aa 1-100.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human SH2D1B aa 1-100. The exact immunogen used to generate this antibody is proprietary information. Database link O14796
Reactivity data
Select an application| IHC-P | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/20 - 1/200 | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50 - 1/200 | Notes - |
Associated Products
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Target data
Function
Cytoplasmic adapter regulating receptors of the signaling lymphocytic activation molecule (SLAM) family such as CD84, SLAMF1, LY9 and CD244 (PubMed:11689425). In SLAM signaling seems to cooperate with SH2D1A/SAP. Plays a role in regulation of effector functions of natural killer (NK) cells by controlling signal transduction through CD244/2B4 without effecting its tyrosine phosphorylation; downstream signaling involves PLCG1 and ERK activation (PubMed:24687958). Activation of SLAMF7-mediated NK cell function does not effect receptor tyrosine phosphorylation but distal signaling (By similarity). In the context of NK cell-mediated cytotoxicity does not enhance conjugate formation with target cells but stimulates polarization of the microtubule-organizing center and cytotoxic granules toward the NK cell synapse (PubMed:24687958). Negatively regulates CD40-induced cytokine production in dendritic cells downstream of SLAM family receptors probably by inducing activation of the PI3K pathway to inhibit p38 MAPK and JNK activation (By similarity).
Alternative names
EAT2, SH2D1B, SH2 domain-containing protein 1B, EWS/FLI1-activated transcript 2, EAT-2
Recommended products
Rabbit Polyclonal SH2D1B antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human SH2D1B aa 1-100.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human SH2D1B aa 1-100. The exact immunogen used to generate this antibody is proprietary information. Database link O14796
- Purification technique
- Affinity purification Protein G
- Concentration
- Purification notes
Purity >95%.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
SH2D1B also known as EAT-2 is an adaptor protein that has a significant role in immune cell signaling. It is about 15 kDa in size and contains a single SH2 domain which allows it to interact with phosphorylated tyrosine-containing proteins. SH2D1B is mainly expressed in natural killer (NK) cells and some T-cell populations. In these cells SH2D1B modulates signaling pathways by interacting with various receptors and other signaling proteins affecting cellular responses.
- Biological function summary
SH2D1B influences immune cell activation and effector functions. It is part of a signaling complex that modulates the activity of NK cells and some T-cells influencing processes like cytotoxicity and cytokine production. By participating in this complex SH2D1B plays a role in regulating immune responses affecting conditions ranging from viral infections to cancer surveillance where adaptive immunity is modified.
- Pathways
SH2D1B is involved in the regulation of the SLAM family receptor signaling pathway which is vital for the activation and function of NK cells. It interacts closely with other proteins in this pathway such as SAP (SLAM-associated protein) to influence signaling cascades that determine cell fate and activity. SH2D1B also participates in the NF-kB signaling pathway further affecting immune responses through modulation of gene expression involved in cell survival and differentiation.
- Associated diseases and disorders
SH2D1B has associations with immune system dysfunctions such as X-linked lymphoproliferative syndrome (XLP). This disorder involves unregulated immune responses and is linked to an overactive or misregulated SLAM receptor signaling where SH2D1B collaborates with SAP. Additionally variations in SH2D1B expression or function have potential links to autoimmune diseases due to its role in regulatory pathways of immune activation and suppression.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SH2D1B antibody (ab222814)
Paraffin-embedded human colon cancer tissue stained for SH2D1B using ab222814 at 1/100 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence - Anti-SH2D1B antibody (ab222814)
HepG2 (human liver hepatocellular carcinoma cell line) cells stained for SH2D1B (green) using ab222814 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488-congugated Goat Anti-Rabbit IgG (H+L) .
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