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Rabbit Polyclonal SH2D3A antibody. Suitable for IHC-P, WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human SH2D3A aa 100-250.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SH2D3A antibody (AB224154), expandable thumbnail
  • Western blot - Anti-SH2D3A antibody (AB224154), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SH2D3A antibody (AB224154), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SH2D3A antibody (AB224154), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SH2D3A antibody (AB224154), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Recombinant Fragment Protein within Human SH2D3A aa 100-250. The exact immunogen used to generate this antibody is proprietary information. Database link Q9BRG2

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWB
Human
Tested
Tested

Tested
Tested

Species

Human

Dilution info

1/20.00000 - 1/50.00000

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Tested
Tested

Species

Human

Dilution info

0.04000-0.40000 µg/mL

Notes

-

Associated Products

Select an associated product type

1 product for Alternative Product

Target data

Function

May play a role in JNK activation.

Alternative names

Recommended products

Rabbit Polyclonal SH2D3A antibody. Suitable for IHC-P, WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human SH2D3A aa 100-250.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Recombinant Fragment Protein within Human SH2D3A aa 100-250. The exact immunogen used to generate this antibody is proprietary information. Database link Q9BRG2
Purification technique

Affinity purification Immunogen

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

SH2D3A also known as NSP3 or CHAT is an adaptor protein that contains an SH2 domain and other structural motifs that facilitate signal transduction. It weighs approximately 65 kDa and is expressed across various tissues including lymphoid tissues and the brain. SH2D3A plays a role in cell signaling by acting as a bridge between receptors and downstream signaling molecules allowing for the modulation of multiple intracellular pathways.

Biological function summary

SH2D3A facilitates important cellular functions such as cytoskeletal reorganization and cell migration. The protein interacts with other signaling proteins and is part of larger complexes involved in cellular movement and structural organization. These interactions are critical for maintaining cellular integrity and function particularly in immune and nervous system tissues.

Pathways

SH2D3A acts within the integrin signaling and PI3K-AKT pathways. It interacts with molecules like GRB2 and IRS1 which are important for transmitting signals that control cell growth and survival. These pathways allow SH2D3A to influence diverse cellular processes including cell adhesion and metabolic regulation by modulating the activity of related proteins.

Associated diseases and disorders

SH2D3A has associations with cancer and autoimmune disorders. Its ability to regulate signaling pathways that control cell migration and invasion links it to tumor progression and metastasis particularly in breast cancer. Additionally SH2D3A interacts with proteins such as FAK and SRC which are known to play a role in cancer progression and also influence immune system signaling suggesting a potential role in autoimmune conditions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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5 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SH2D3A antibody (ab224154), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SH2D3A antibody (ab224154)

    Immunohistochemical analysis of Formalin/PFA-fixed paraffin-embedded sections human small intestine tissue labeling SH2D3A with ab224154 at 1/20. Antigen retrieval was heat mediated with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Western blot - Anti-SH2D3A antibody (ab224154), expandable thumbnail

    Western blot - Anti-SH2D3A antibody (ab224154)

    All lanes: Western blot - Anti-SH2D3A antibody (ab224154) at 1/100 dilution

    All lanes: RT4 (human urinary bladder cancer cell line) cell lysate

    Developed using the ECL technique.

    Predicted band size: 63 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SH2D3A antibody (ab224154), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SH2D3A antibody (ab224154)

    Immunohistochemical analysis of Formalin/PFA-fixed paraffin-embedded sections human skin tissue labeling SH2D3A with ab224154 at 1/20. Antigen retrieval was heat mediated with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SH2D3A antibody (ab224154), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SH2D3A antibody (ab224154)

    Immunohistochemical analysis of Formalin/PFA-fixed paraffin-embedded sections human Fallopian tube tissue labeling SH2D3A with ab224154 at 1/20. Antigen retrieval was heat mediated with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SH2D3A antibody (ab224154), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SH2D3A antibody (ab224154)

    Immunohistochemical analysis of Formalin/PFA-fixed paraffin-embedded sections human liver tissue labeling SH2D3A with ab224154 at 1/20. Antigen retrieval was heat mediated with citrate buffer pH 6 before commencing with IHC staining protocol.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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