Rabbit Polyclonal SH3BGRL3 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human SH3BGRL3 aa 1 to C-terminus.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human SH3BGRL3 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q9H299
Reactivity data
Select an application| IHC-P | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/600 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.04000-0.40000 µg/mL | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1.00000-4.00000 µg/mL | Notes Fixed/permeabilized with PFA/Triton X-100 |
Target data
Function
Could act as a modulator of glutaredoxin biological activity (Probable). May play a role in cytoskeleton organization (PubMed:34380438).
Alternative names
P1725, SH3BGRL3, SH3 domain-binding glutamic acid-rich-like protein 3, SH3 domain-binding protein 1, TNF inhibitory protein B1, SH3BP-1, TIP-B1
Recommended products
Rabbit Polyclonal SH3BGRL3 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human SH3BGRL3 aa 1 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human SH3BGRL3 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q9H299
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
SH3BGRL3 also known as SH3 domain binding glutamic acid-rich protein-like 3 is a small protein with a molecular mass of approximately 17 kDa. This protein is expressed in a variety of tissues including the liver heart and muscle. It contains an SH3 binding domain known to interact with SH3 domains of other proteins providing a regulatory mechanism in cell signaling. The protein is also highly expressed in certain tumor cells indicating its potential role in cellular transformation and cancer.
- Biological function summary
The protein is involved in several cellular processes including signal transduction and cytoskeleton organization. SH3BGRL3 functions as part of a protein complex that modulates interactions between signaling molecules and cytoskeletal components. It plays an important role in maintaining cellular architecture and facilitating cell communication by interacting with other proteins inside the cell. This interaction can affect cell structure and growth.
- Pathways
This protein participates in signal transduction and cytoskeletal dynamics pathways. It interacts with proteins such as Src which is involved in various signaling cascades. SH3BGRL3's connection to the Src signaling pathway is significant as it can modulate processes like cell proliferation and differentiation. The protein is also linked with actin cytoskeleton pathways further highlighting its role in maintaining cell structure integrity.
- Associated diseases and disorders
Research connects SH3BGRL3 to cancer and cardiovascular diseases. Its expression levels in tumor cells are linked to cancer progression potentially serving as a biomarker for tumor development. In cardiovascular disorders alterations in SH3BGRL3 can affect heart muscle function possibly influencing disease outcomes. The protein has associations with Src in both cancer and heart diseases suggesting a broader role in disease mechanisms through these pathways.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Western blot - Anti-SH3BGRL3 antibody (ab122138)
All lanes: Western blot - Anti-SH3BGRL3 antibody (ab122138) at 1/250 dilution
Lane 1: RT4 cell lysates
Lane 2: U251MG cell lysates
Lane 3: Human plasma
Lane 4: Human liver tissue lysate
Lane 5: Human tonsil tissue lysate
Predicted band size: 10 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SH3BGRL3 antibody (ab122138)
ab122138, at 1/600 dilution, staining SH3BGRL3 in Human smooth muscle tissue by Immunohistochemistry using paraffin-embedded tissue.
Immunocytochemistry/ Immunofluorescence - Anti-SH3BGRL3 antibody (ab122138)
ab122138 at 4 μg/ml staining SH3BGRL3 in PFA/Triton X-100 fixed/permeabilized U2OS cells by Immunofluorescence. Antibody staining is shown in green
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