Rabbit Polyclonal SH3BP1 antibody. Suitable for WB, IHC-P and reacts with Human, Mouse samples. Immunogen corresponding to Recombinant Fragment Protein within Human SH3BP1.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human SH3BP1. The exact immunogen used to generate this antibody is proprietary information. Database link Q9Y3L3
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Expected |
| Mouse | Expected | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Target data
Function
GTPase activating protein (GAP) which specifically converts GTP-bound Rho-type GTPases including RAC1 and CDC42 in their inactive GDP-bound form. By specifically inactivating RAC1 at the leading edge of migrating cells, it regulates the spatiotemporal organization of cell protrusions which is important for proper cell migration (PubMed:21658605). Also negatively regulates CDC42 in the process of actin remodeling and the formation of epithelial cell junctions (PubMed:22891260). Through its GAP activity toward RAC1 and/or CDC42 plays a specific role in phagocytosis of large particles. Specifically recruited by a PI3 kinase/PI3K-dependent mechanism to sites of large particles engagement, inactivates RAC1 and/or CDC42 allowing the reorganization of the underlying actin cytoskeleton required for engulfment (PubMed:26465210). It also plays a role in angiogenesis and the process of repulsive guidance as part of a semaphorin-plexin signaling pathway. Following the binding of PLXND1 to extracellular SEMA3E it dissociates from PLXND1 and inactivates RAC1, inducing the intracellular reorganization of the actin cytoskeleton and the collapse of cells (PubMed:24841563).
Alternative names
SH3 domain-binding protein 1, SH3BP1
Recommended products
Rabbit Polyclonal SH3BP1 antibody. Suitable for WB, IHC-P and reacts with Human, Mouse samples. Immunogen corresponding to Recombinant Fragment Protein within Human SH3BP1.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human SH3BP1. The exact immunogen used to generate this antibody is proprietary information. Database link Q9Y3L3
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
SH3BP1 also known as SH3-domain binding protein 1 is a multifunctional protein with a molecular mass of approximately 79 kDa. It plays a significant role in cellular dynamics particularly in the regulation of actin cytoskeleton organization and cell migration. SH3BP1 interacts with small GTPases like Rac1 by acting as a GTPase-activating protein (GAP) which means it helps in the conversion of the active form of Rac1 to an inactive GDP-bound form. This protein is expressed in various tissues across the human body including kidney liver and muscle tissues indicating its broad physiological relevance.
- Biological function summary
SH3BP1 contributes to the regulation of cell shape and movement. It acts as a part of complexes associated with actin cytoskeleton remodeling which is important for the proper functioning of membrane dynamics. The ability of SH3BP1 to bind and regulate different components involved in this process positions it as an important mediator of cytoskeletal reorganization. This mechanism aids in cellular responses to external stimuli facilitating processes such as cell adhesion and migration.
- Pathways
SH3BP1 is involved in the regulation of the Rho GTPase signaling pathway. This pathway is essential for controlling cytoskeletal dynamics and cellular morphogenesis. In this context SH3BP1 works closely with proteins like Rac1 and RhoA to modulate these signaling cascades effectively. Additionally SH3BP1 interplays with the integrin signaling pathway further influencing cell movement and adhesion by interacting with focal adhesion kinase (FAK).
- Associated diseases and disorders
SH3BP1 has associations with cancers such as breast cancer due to its role in cell migration and invasion. The dysregulation of pathways involving SH3BP1 can lead to alterations in cell adhesion and metastasis. Its interaction with Rac1 and other related proteins like PAK1 contributes to altered signaling that may promote tumorigenesis. Additionally altered expression levels of SH3BP1 have been implicated in neurological disorders such as autism spectrum disorders possibly through its influence on synaptic function and neuronal connectivity.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Western blot - Anti-SH3BP1 antibody (ab227548)
7.5% SDS-PAGE
All lanes: Western blot - Anti-SH3BP1 antibody (ab227548) at 1/1000 dilution
All lanes: A431 (human epidermoid carcinoma cell line) whole cell lysate at 30 µg
Developed using the ECL technique.
Predicted band size: 76 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SH3BP1 antibody (ab227548)
Paraffin-embedded mouse duodenum tissue stained for SH3BP1 with ab227548 at 1/500 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SH3BP1 antibody (ab227548)
Paraffin-embedded MDA-MB-157 tumor xenograft tissue stained for SH3BP1 with ab227548 at 1/250 dilution in immunohistochemical analysis.
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