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AB227548

Anti-SH3BP1 antibody

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(1 Publication)

Rabbit Polyclonal SH3BP1 antibody. Suitable for WB, IHC-P and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human SH3BP1.

View Alternative Names

SH3 domain-binding protein 1, SH3BP1

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SH3BP1 antibody (AB227548)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SH3BP1 antibody (AB227548)

Paraffin-embedded MDA-MB-157 tumor xenograft tissue stained for SH3BP1 with ab227548 at 1/250 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SH3BP1 antibody (AB227548)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SH3BP1 antibody (AB227548)

Paraffin-embedded mouse duodenum tissue stained for SH3BP1 with ab227548 at 1/500 dilution in immunohistochemical analysis.

Western blot - Anti-SH3BP1 antibody (AB227548)
  • WB

Supplier Data

Western blot - Anti-SH3BP1 antibody (AB227548)

7.5% SDS-PAGE

All lanes:

Western blot - Anti-SH3BP1 antibody (ab227548) at 1/1000 dilution

All lanes:

A431 (human epidermoid carcinoma cell line) whole cell lysate at 30 µg

Predicted band size: 76 kDa

true

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human SH3BP1. The exact immunogen used to generate this antibody is proprietary information.

Q9Y3L3

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SH3BP1 also known as SH3-domain binding protein 1 is a multifunctional protein with a molecular mass of approximately 79 kDa. It plays a significant role in cellular dynamics particularly in the regulation of actin cytoskeleton organization and cell migration. SH3BP1 interacts with small GTPases like Rac1 by acting as a GTPase-activating protein (GAP) which means it helps in the conversion of the active form of Rac1 to an inactive GDP-bound form. This protein is expressed in various tissues across the human body including kidney liver and muscle tissues indicating its broad physiological relevance.
Biological function summary

SH3BP1 contributes to the regulation of cell shape and movement. It acts as a part of complexes associated with actin cytoskeleton remodeling which is important for the proper functioning of membrane dynamics. The ability of SH3BP1 to bind and regulate different components involved in this process positions it as an important mediator of cytoskeletal reorganization. This mechanism aids in cellular responses to external stimuli facilitating processes such as cell adhesion and migration.

Pathways

SH3BP1 is involved in the regulation of the Rho GTPase signaling pathway. This pathway is essential for controlling cytoskeletal dynamics and cellular morphogenesis. In this context SH3BP1 works closely with proteins like Rac1 and RhoA to modulate these signaling cascades effectively. Additionally SH3BP1 interplays with the integrin signaling pathway further influencing cell movement and adhesion by interacting with focal adhesion kinase (FAK).

SH3BP1 has associations with cancers such as breast cancer due to its role in cell migration and invasion. The dysregulation of pathways involving SH3BP1 can lead to alterations in cell adhesion and metastasis. Its interaction with Rac1 and other related proteins like PAK1 contributes to altered signaling that may promote tumorigenesis. Additionally altered expression levels of SH3BP1 have been implicated in neurological disorders such as autism spectrum disorders possibly through its influence on synaptic function and neuronal connectivity.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

GTPase activating protein (GAP) which specifically converts GTP-bound Rho-type GTPases including RAC1 and CDC42 in their inactive GDP-bound form. By specifically inactivating RAC1 at the leading edge of migrating cells, it regulates the spatiotemporal organization of cell protrusions which is important for proper cell migration (PubMed : 21658605). Also negatively regulates CDC42 in the process of actin remodeling and the formation of epithelial cell junctions (PubMed : 22891260). Through its GAP activity toward RAC1 and/or CDC42 plays a specific role in phagocytosis of large particles. Specifically recruited by a PI3 kinase/PI3K-dependent mechanism to sites of large particles engagement, inactivates RAC1 and/or CDC42 allowing the reorganization of the underlying actin cytoskeleton required for engulfment (PubMed : 26465210). It also plays a role in angiogenesis and the process of repulsive guidance as part of a semaphorin-plexin signaling pathway. Following the binding of PLXND1 to extracellular SEMA3E it dissociates from PLXND1 and inactivates RAC1, inducing the intracellular reorganization of the actin cytoskeleton and the collapse of cells (PubMed : 24841563).
See full target information SH3BP1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cellular physiology and biochemistry : internation 50:136-149 PubMed30278449

2018

miR-449a Suppresses Tamoxifen Resistance in Human Breast Cancer Cells by Targeting ADAM22.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Li,Mingjie Lu,Jiao Jin,Xiyi Lu,Tongpeng Xu,Shidai Jin
View all publications
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