JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB126231

Anti-SH3BP5 antibody

Be the first to review this product! Submit a review

|

(1 Publication)

Rabbit Polyclonal SH3BP5 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human SH3BP5 aa 1-250.

View Alternative Names

SAB, SH3BP5, SH3 domain-binding protein 5, SH3BP-5, SH3 domain-binding protein that preferentially associates with BTK

1 Images
Western blot - Anti-SH3BP5 antibody (AB126231)
  • WB

Unknown

Western blot - Anti-SH3BP5 antibody (AB126231)

12% SDS PAGE

All lanes:

Western blot - Anti-SH3BP5 antibody (ab126231) at 1/1000 dilution

All lanes:

THP1 whole cell lysate at 30 µg

Predicted band size: 118 kDa,50 kDa,56 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Recombinant Fragment Protein within Human SH3BP5 aa 1-250. The exact immunogen used to generate this antibody is proprietary information.

O60239

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SH3BP5 also known as SH3-domain binding protein 5 or Bnk is a protein with a molecular weight of approximately 51 kDa. It is expressed in various tissues including the brain liver and heart. This protein negatively regulates protein kinase C (PKC) signaling by inhibiting its phosphorylation. SH3BP5 has a modular structure that allows it to interact with specific SH3 domains enabling it to participate in diverse signaling processes within the cell.
Biological function summary

SH3BP5 plays a significant role in cellular signal transduction networks. It acts as an adaptor protein facilitating interactions between different signaling molecules. SH3BP5 is known to be an important part of complexes that regulate both cellular development and stress responses. By interacting with different partners it influences processes like cell growth differentiation and response to external stimuli.

Pathways

SH3BP5 integrates its function within key signaling pathways such as the mitogen-activated protein kinase (MAPK) pathway and the PKC pathway. These pathways are essential for various cellular functions including gene expression and cellular proliferation. SH3BP5 interacts with proteins like RAF-1 in the MAPK pathway modulating downstream signaling events. It also associates with PKC isoforms affecting their activity and stability in cells.

SH3BP5 has connections to neurological disorders and cancer. Alterations in SH3BP5 expression or function can disrupt normal signaling pathways contributing to the development and progression of these conditions. In cancer SH3BP5's connection to PKC and RAF-1 pathways highlights its potential role in oncogenic processes. In neurological disorders its involvement in neuronal signaling suggests a potential impact on diseases involving neuronal communication. Understanding these associations could provide valuable insights into therapeutic strategies.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Functions as a guanine nucleotide exchange factor (GEF) with specificity for RAB11A and RAB25 (PubMed : 26506309, PubMed : 30217979). Inhibits the auto- and transphosphorylation activity of BTK. Plays a negative regulatory role in BTK-related cytoplasmic signaling in B-cells. May be involved in BCR-induced apoptotic cell death.
See full target information SH3BP5
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in cell and developmental biology 12:1396890 PubMed38983788

2024

Screening of potentially active compounds against rheumatoid arthritis in the Juan-Bi decoction using systems pharmacology and animal experiments.

Applications

Unspecified application

Species

Unspecified reactive species

Dahai Liu,Qiang Fu,Leyna G Liu,Wenwen Li,Fei Qi,Justin Liu,Lifeng Shang,Xiu Wang,Fang Yang,Jie Li,Daoqiang Lu,Huiying Feng,Ziwen Zhang,Yiqing Chen,Junru Liang,Jiayi Yao,Hua Lv,Riwang Li,Jun Wang,Di Wu,Yuxi Liu,Chenglai Xia,Wenxing Li
View all publications
style
left-column
style
left-column
style
right-column

Alternative Products

Primary Antibodies

AB222860

Anti-SH3BP5 antibody

Immunocytochemistry/ Immunofluorescence - Anti-SH3BP5 antibody (AB222860)

  • 0
  • 0

Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB23395

Anti-N WASP (phospho Y256) antibody

Western blot - Anti-N WASP (phospho Y256) antibody (AB23395)

  • 0
  • 0
Primary Antibodies

AB96551

Anti-MAP4K5 antibody

Immunocytochemistry/ Immunofluorescence - Anti-MAP4K5 antibody (AB96551)

  • 4
  • 1
Primary Antibodies

AB111697

Anti-ZNF587 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZNF587 antibody (AB111697)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com