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AB222860

Anti-SH3BP5 antibody

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(1 Publication)

Rabbit Polyclonal SH3BP5 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human SH3BP5 aa 1 to C-terminus.

View Alternative Names

SAB, SH3BP5, SH3 domain-binding protein 5, SH3BP-5, SH3 domain-binding protein that preferentially associates with BTK

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-SH3BP5 antibody (AB222860)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SH3BP5 antibody (AB222860)

HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for SH3BP5 (green) using ab222860 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488-congugated Goat Anti-Rabbit IgG (H+L).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SH3BP5 antibody (AB222860)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SH3BP5 antibody (AB222860)

Paraffin-embedded human ovarian cancer tissue stained for SH3BP5 using ab222860 at 1/100 dilution in immunohistochemical analysis.

Western blot - Anti-SH3BP5 antibody (AB222860)
  • WB

Supplier Data

Western blot - Anti-SH3BP5 antibody (AB222860)

All lanes:

Western blot - Anti-SH3BP5 antibody (ab222860) at 1/2000 dilution

Lane 1:

Mouse heart lysate

Lane 2:

Mouse brain lysate

Lane 3:

Mouse kidney lysate

Predicted band size: 50 kDa

Observed band size: 51 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human SH3BP5 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

O60239

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purity >95%.
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SH3BP5 also known as SH3-domain binding protein 5 or Bnk is a protein with a molecular weight of approximately 51 kDa. It is expressed in various tissues including the brain liver and heart. This protein negatively regulates protein kinase C (PKC) signaling by inhibiting its phosphorylation. SH3BP5 has a modular structure that allows it to interact with specific SH3 domains enabling it to participate in diverse signaling processes within the cell.
Biological function summary

SH3BP5 plays a significant role in cellular signal transduction networks. It acts as an adaptor protein facilitating interactions between different signaling molecules. SH3BP5 is known to be an important part of complexes that regulate both cellular development and stress responses. By interacting with different partners it influences processes like cell growth differentiation and response to external stimuli.

Pathways

SH3BP5 integrates its function within key signaling pathways such as the mitogen-activated protein kinase (MAPK) pathway and the PKC pathway. These pathways are essential for various cellular functions including gene expression and cellular proliferation. SH3BP5 interacts with proteins like RAF-1 in the MAPK pathway modulating downstream signaling events. It also associates with PKC isoforms affecting their activity and stability in cells.

SH3BP5 has connections to neurological disorders and cancer. Alterations in SH3BP5 expression or function can disrupt normal signaling pathways contributing to the development and progression of these conditions. In cancer SH3BP5's connection to PKC and RAF-1 pathways highlights its potential role in oncogenic processes. In neurological disorders its involvement in neuronal signaling suggests a potential impact on diseases involving neuronal communication. Understanding these associations could provide valuable insights into therapeutic strategies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Functions as a guanine nucleotide exchange factor (GEF) with specificity for RAB11A and RAB25 (PubMed : 26506309, PubMed : 30217979). Inhibits the auto- and transphosphorylation activity of BTK. Plays a negative regulatory role in BTK-related cytoplasmic signaling in B-cells. May be involved in BCR-induced apoptotic cell death.
See full target information SH3BP5
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of inflammation research 17:11469-11483 PubMed39735896

2024

Macrophage Infiltration Correlated with IFI16, EGR1 and MX1 Expression in Renal Tubular Epithelial Cells Within Lupus Nephritis-Associated Tubulointerstitial Injury via Bioinformatics Analysis.

Applications

Unspecified application

Species

Unspecified reactive species

Ming Tian,Min Tang,Caiming Chen,Yufang Lin,Hong Chen,Yanfang Xu
View all publications
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Product promise

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