AB317836

Anti-SH3GL1/Endophilin A2 antibody [K51/1] - BSA and Azide free

BOND RX™ Validated
Recombinant
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Mouse Recombinant Monoclonal SH3G1 antibody. Carrier free. Suitable for WB, IHC-P, IP and reacts with Rat samples.

View Alternative Names

Sh3p8, Endophilin-A2, Endophilin-2, SH3 domain protein 2B, SH3 domain-containing GRB2-like protein 1, SH3p8

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SH3GL1/Endophilin A2 antibody [K51/1] - BSA and Azide free (AB317836)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SH3GL1/Endophilin A2 antibody [K51/1] - BSA and Azide free (AB317836)

This data was developed using ab317835, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Rat cardiac muscle tissue labeling SH3GL1/Endophilin A2 with ab317835 at 1/1000 (1.094 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Low expression : weak staining on rat cardiac muscle.
The section was incubated with ab317835 for 30 mins at room temperature, followed by anti-mouse IgG1 antibody (ab125913) for 8 mins during the LeicaDS9800 kit staining procedure.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SH3GL1/Endophilin A2 antibody [K51/1] - BSA and Azide free (AB317836)

This data was developed using ab317835, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling SH3GL1/Endophilin A2 with ab317835 at 1/1000 (1.094 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Cytoplasmic staining on rat cerebrum.
The section was incubated with ab317835 for 30 mins at room temperature, followed by anti-mouse IgG1 antibody (ab125913) for 8 mins during the LeicaDS9800 kit staining procedure.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Supplier Data

Immunoprecipitation - Anti-SH3GL1/Endophilin A2 antibody [K51/1] - BSA and Azide free (AB317836)

This data was developed using ab317835, the same antibody clone in a different buffer formulation.

SH3GL1/Endophilin A2 was immunoprecipitated from 0.35 mg Rat testis tissue lysate with ab317835 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab317835 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : Rat testis tissue lysate
Lane 2 : ab317835 IP in Rat testis tissue lysate
Lane 3 : Mouse monoclonal IgG (ab18443) instead of ab317835 in rat testis tissue lysate

All lanes:

Immunoprecipitation - Anti-SH3GL1/Endophilin A2 antibody [K51/1] (<a href='/en-us/products/primary-antibodies/sh3gl1-endophilin-a2-antibody-k51-1-ab317835'>ab317835</a>) at 1/30 dilution

All lanes:

Rat testis tissue lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

false

Exposure time: 41s

Supplier Data

Western blot - Anti-SH3GL1/Endophilin A2 antibody [K51/1] - BSA and Azide free (AB317836)

This data was developed using ab317835, the same antibody clone in a different buffer formulation.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-SH3GL1/Endophilin A2 antibody [K51/1] (<a href='/en-us/products/primary-antibodies/sh3gl1-endophilin-a2-antibody-k51-1-ab317835'>ab317835</a>) at 1/1000 dilution

Lane 1:

PC-12 (rat adrenal gland pheochromocytoma cell) whole cell lysate at 20 µg

Lane 2:

C6 (rat glial tumor glial cell) whole cell lysate at 20 µg

Lane 3:

L6 (rat skeletal muscle myoblast) whole cell lysate at 20 µg

Lane 4:

AR42J (rat pancreatic tumor epithelial cell) whole cell lysate at 20 µg

Lane 5:

H9c2(2-1) (rat cardiomyoblast) whole cell lysate at 20 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

Observed band size: 41 kDa,36 kDa

false

Exposure time: 180s

Supplier Data

Western blot - Anti-SH3GL1/Endophilin A2 antibody [K51/1] - BSA and Azide free (AB317836)

This data was developed using ab317835, the same antibody clone in a different buffer formulation.

Low expression : heart and kidney (PMID : 11518713).

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-SH3GL1/Endophilin A2 antibody [K51/1] (<a href='/en-us/products/primary-antibodies/sh3gl1-endophilin-a2-antibody-k51-1-ab317835'>ab317835</a>) at 1/1000 dilution

Lane 1:

Rat brain tissue lysate at 20 µg

Lane 2:

Rat heart tissue lysate at 20 µg

Lane 3:

Rat kidney tissue lysate at 20 µg

Lane 4:

Rat spleen tissue lysate at 20 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

Observed band size: 41 kDa,36 kDa

false

Exposure time: 180s

Unconjugated

Anti-SH3GL1/Endophilin A2 antibody [K51/1]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

K51/1

Isotype

IgG1

Carrier free

Yes

Reacts with

Rat

Applications

WB, IHC-P, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Mouse": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "" } } }

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Product details

ab317836 is the carrier-free version of ab317835.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SH3GL1 also known as Endophilin A2 is a protein that facilitates membrane curvature during endocytosis through its BAR domain. It weighs approximately 42 kDa. Endophilin A2 is widely expressed in various tissues with notable abundance in the brain. It functions by binding to membrane lipids and interacting with other endocytic proteins. This contributes to the formation of vesicles that ferry molecules into cells vital for nutrient uptake and receptor recycling.

Biological function summary

SH3GL1/Endophilin A2 participates in synaptic vesicle endocytosis a process critical for neurotransmission and proper brain function. It works in association with other proteins in a complex such as dynamin to mediate rapid membrane invagination and fission. By assisting in vesicle formation and release at synaptic terminals it plays a role in maintaining synaptic efficacy and neuronal communication.

Pathways

This protein plays a significant role in the clathrin-mediated endocytosis and synaptic vesicle cycle pathways. In these contexts Endophilin A2 interacts with proteins like clathrin and amphiphysin to enable efficient vesicle budding and release. Its activities connect to the recycling of synaptic vesicles essential for the preservation of synaptic transmission and cell signaling.

SH3GL1/Endophilin A2 associates with neurodegenerative diseases like Alzheimer's disease. Dysfunctional endocytosis resulting from its altered expression or activity can impair synaptic function contributing to disease progression. Other disorders involving this protein include certain types of cancer where its interactions with proteins such as c-Cbl may impact cell proliferation and survival influencing tumorigenesis.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Implicated in endocytosis. May recruit other proteins to membranes with high curvature (By similarity).

See full target information Sh3gl1

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SH3GL1/Endophilin A2 antibody [K51/1] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SH3GL1/Endophilin A2 antibody [K51/1] - BSA and Azide free (AB317836)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SH3GL1/Endophilin A2 antibody [K51/1] - BSA and Azide free (AB317836)

Immunoprecipitation - Anti-SH3GL1/Endophilin A2 antibody [K51/1] - BSA and Azide free (AB317836)

Western blot - Anti-SH3GL1/Endophilin A2 antibody [K51/1] - BSA and Azide free (AB317836)

Western blot - Anti-SH3GL1/Endophilin A2 antibody [K51/1] - BSA and Azide free (AB317836)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Product promise