AB248987

Anti-SH3GL1 + SH3GL2 + SH3GL3 antibody [EPR10215(B)] - BSA and Azide free

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal SH3GL2 antibody. Carrier free. Suitable for WB and reacts with Recombinant fragment - Human, Human, Mouse, Rat samples. Cited in 1 publication.

View Alternative Names

CNSA2, SH3D2A, SH3GL2, Endophilin-A1, EEN-B1, Endophilin-1, SH3 domain protein 2A, SH3 domain-containing GRB2-like protein 2, CNSA1, SH3D2B, Endophilin-A2, EEN fusion partner of MLL, Endophilin-2, Extra eleven-nineteen leukemia fusion gene protein, SH3 domain protein 2B, SH3 domain-containing GRB2-like protein 1, EEN, CNSA3, SH3D2C, SH3GL3, Endophilin-A3, EEN-B2, Endophilin-3, SH3 domain protein 2C, SH3 domain-containing GRB2-like protein 3

2 Images

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Western blot - Anti-SH3GL1 + SH3GL2 + SH3GL3 antibody [EPR10215(B)] - BSA and Azide free (AB248987)

This data was developed using ab150431, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-SH3GL1 + SH3GL2 + SH3GL3 antibody [EPR10215(B)] (<a href='/en-us/products/primary-antibodies/sh3gl1-sh3gl2-sh3gl3-antibody-epr10215b-ab150431'>ab150431</a>) at 1/10000 dilution

Lane 1:

Human cerebellum lysate at 10 µg

Lane 2:

Mouse brain lysate at 10 µg

Lane 3:

Rat brain lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 40 kDa,52 kDa

false

Lab

Western blot - Anti-SH3GL1 + SH3GL2 + SH3GL3 antibody [EPR10215(B)] - BSA and Azide free (AB248987)

This data was developed using ab150431, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-SH3GL1 + SH3GL2 + SH3GL3 antibody [EPR10215(B)] (<a href='/en-us/products/primary-antibodies/sh3gl1-sh3gl2-sh3gl3-antibody-epr10215b-ab150431'>ab150431</a>) at 1/100 dilution

Lane 1:

Human SH3GL3 recombinant protein (aa1-347) without tag at 10 ng

Lane 2:

Human SH3GL2 recombinant protein (aa1-352) without tag at 10 ng

Lane 3:

Human SH3GL1 recombinant protein (aa1-368) without tag at 10 ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 40-50 kDa

false

Exposure time: 6s

Unconjugated

Anti-SH3GL1 + SH3GL2 + SH3GL3 antibody [EPR10215(B)]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR10215(B)

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Recombinant fragment - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

ab248987 is the carrier-free version of ab150431.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SH3GL2 also known as Endophilin A1 is a protein that plays an important role in membrane dynamics during endocytosis. It has a molecular weight of approximately 41 kDa. Mechanically SH3GL2 binds to lipids and helps in membrane curvature due to its BAR domain. This protein is highly expressed in neuronal tissues where it participates in synaptic vesicle endocytosis. It interacts with other proteins via its SH3 domain facilitating the assembly of protein complexes involved in these cellular processes.

Biological function summary

SH3GL2 is involved in synaptic vesicle recycling an important process for neurocommunication. It is part of a complex that includes proteins such as dynamin and synaptojanin. It ensures the rapid turnover of synaptic vesicles by mediating their endocytosis and recycling back to the synaptic terminal. This function helps in maintaining efficient synaptic transmission essential for neuronal communication and plasticity.

Pathways

SH3GL2 is involved in the clathrin-mediated endocytosis pathway. This pathway is critical for the retrieval of synaptic vesicles in neurons. SH3GL2 works closely with clathrin and dynamin to facilitate the budding and scission of vesicles from the plasma membrane. In addition SH3GL2 also interacts within the synaptic vesicle cycle ensuring the availability of vesicles for neurotransmitter release.

SH3GL2 has been linked to neurodegenerative diseases such as Parkinson's disease. Its interaction with proteins like alpha-synuclein suggests its role in the pathogenesis of this disorder. Altered SH3GL2 function may disrupt normal synaptic vesicle dynamics contributing to neuronal dysfunction. Furthermore aberrant SH3GL2 expression or function may also be involved in schizoaffective disorders where its interaction with synaptojanin plays a role in synaptic dysfunction.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Implicated in synaptic vesicle endocytosis. May recruit other proteins to membranes with high curvature. Required for BDNF-dependent dendrite outgrowth. Cooperates with SH3GL2 to mediate BDNF-NTRK2 early endocytic trafficking and signaling from early endosomes.

See full target information SH3GL2

Additional targets

SH3GL1,SH3GL3

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Oncology reports 50: PubMed37732539

2023

Heat shock protein B7 (HSPB7) inhibits lung adenocarcinoma progression by inhibiting glycolysis.

Applications

Unspecified application

Species

Unspecified reactive species

Zhitao Chen,Peipei Li,Lingguang Shen,Xiuyu Jiang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SH3GL1 + SH3GL2 + SH3GL3 antibody [EPR10215(B)] - BSA and Azide free

Western blot - Anti-SH3GL1 + SH3GL2 + SH3GL3 antibody [EPR10215(B)] - BSA and Azide free (AB248987)

Western blot - Anti-SH3GL1 + SH3GL2 + SH3GL3 antibody [EPR10215(B)] - BSA and Azide free (AB248987)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Additional targets

Publications (1)

Product promise