Anti-SH3GL3 antibody [EPR29565-553]

9 product images

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  Western blot - Anti-SH3GL3 antibody [EPR29565-553] (ab323569)

  Blocking and diluting buffer and concentration: 5% NFDM/TBST.

  This antibody does not cross-react with recombinant human SH3GL2 or SH3GL1 by western blot.

  In Western blot, Anti-SH3GL1 + SH3GL2 + SH3GL3 antibody [EPR10215(B)] - (Anti-SH3GL1 + SH3GL2 + SH3GL3 antibody [EPR10215(B)] ab150431) - total protein Control staining at 1/1000 dilution.

  All lanes: Western blot - Anti-SH3GL3 antibody [EPR29565-553] (ab323569) at 1/1000 dilution

  Lane 1: Human SH3GL3 recombinant protein (no-tag) at 10 ng

  Lane 2: Human SH3GL2 recombinant protein (no-tag) at 10 ng

  Lane 3: Human SH3GL1 recombinant protein (no-tag) at 10 ng

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

  Observed band size: 39,32 kDa, 40 kDa, 42 kDa

  Exposure time: 8s

• 

  Immunocytochemistry/ Immunofluorescence - Anti-SH3GL3 antibody [EPR29565-553] (ab323569)

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Neuro-2a (mouse neuroblastoma neuroblast) cells labelling SH3GL3 with ab323569 at 1/100 (4.9 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/ 1000 2ug/ml dilution (Green).

  Confocal image showing cytoplasmic and membranous staining in Neuro-2a cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue).
  
  Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
  
  Negative control: A20.

  Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

  Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/ 1000 2ug/ml dilution.

• 

  Western blot - Anti-SH3GL3 antibody [EPR29565-553] (ab323569)

  Blocking and diluting buffer and concentration: 5% NFDM/TBST.

  Negative control: heart, liver (PMID: 9809064).

  Low expression: A20, B16-F10.

  The identity of the bands higher than 50 kDa are unknown.

  In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

  All lanes: Western blot - Anti-SH3GL3 antibody [EPR29565-553] (ab323569) at 1/1000 dilution

  Lane 1: Mouse brain tissue lysate at 20 µg

  Lane 2: Mouse testis tissue lysate at 20 µg

  Lane 3: Mouse heart tissue lysate at 20 µg

  Lane 4: Mouse liver tissue lysate at 20 µg

  Lane 5: Mouse striatum tissue lysate at 20 µg

  Lane 6: Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate at 20 µg

  Lane 7: A20 (mouse reticulum sarcoma b lymphocyte) whole cell lysate at 20 µg

  Lane 8: B16-F10 (mouse skin melanoma cell) whole cell lysate at 20 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

  Exposure time: 48s

• 

  Western blot - Anti-SH3GL3 antibody [EPR29565-553] (ab323569)

  Blocking and diluting buffer and concentration: 5% NFDM/TBST.

  Negative control: liver (PMID: 9809064)

  Low expression: HeLa, SU-DHL-1.

  Lanes 3-8 are incubated with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 and lanes 1-2 are incubated with Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000.

  In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

  All lanes: Western blot - Anti-SH3GL3 antibody [EPR29565-553] (ab323569) at 1/1000 dilution

  Lane 1: Human striatum tissue lysate at 20 µg

  Lane 2: Human liver tissue lysate at 20 µg

  Lane 3: SK-N-MC (human brain epithelial cell) whole cell lysate at 20 µg

  Lane 4: HEK-293 (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

  Lane 5: K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate at 20 µg

  Lane 6: A-673 (human muscle ewings sarcoma cell line) whole cell lysate at 20 µg

  Lane 7: SU-DHL-1 (human histiocytic lymphoblast-like cell) whole cell lysate at 20 µg

  Lane 8: HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg

  Secondary

  Lanes 1 - 2: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

  Lanes 3 - 8: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

  Observed band size: 39 kDa, 32 kDa, 36 kDa

  Exposure time: 59s

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SH3GL3 antibody [EPR29565-553] (ab323569)

  Immunohistochemical analysis of paraffin-embedded Mouse testis tissue labeling SH3GL3 with ab323569 at 1/100 (4.9 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Positive staining in mouse testis.
  
  The section was incubated with ab323569 for 30 mins at room temperature.
  
  The immunostaining was performed on a Leica Biosystems BOND® RX instrument

  Counterstained with Hematoxylin.

  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SH3GL3 antibody [EPR29565-553] (ab323569)

  Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling SH3GL3 with ab323569 at 1/100 (4.9 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Negative control: No staining in mouse liver (PMID: 9809064).
  
  The section was incubated with ab323569 for 30 mins at room temperature.
  
  The immunostaining was performed on a Leica Biosystems BOND® RX instrument

  Counterstained with Hematoxylin.

  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SH3GL3 antibody [EPR29565-553] (ab323569)

  Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling SH3GL3 with ab323569 at 1/100 (4.9 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Negative control: No staining in human liver (PMID: 9809064).
  
  The section was incubated with ab323569 for 30 mins at room temperature.
  
  The immunostaining was performed on a Leica Biosystems BOND® RX instrument

  Counterstained with Hematoxylin.

  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SH3GL3 antibody [EPR29565-553] (ab323569)

  Immunohistochemical analysis of paraffin-embedded Human astrocytoma tissue labeling SH3GL3 with ab323569 at 1/100 (4.9 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Positive staining in human astrocytoma.
  
  The section was incubated with ab323569 for 30 mins at room temperature.
  
  The immunostaining was performed on a Leica Biosystems BOND® RX instrument

  Counterstained with Hematoxylin.

  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SH3GL3 antibody [EPR29565-553] (ab323569)

  Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling SH3GL3 with ab323569 at 1/100 (4.9 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Positive staining in mouse cerebrum.
  
  The section was incubated with ab323569 for 30 mins at room temperature.
  
  The immunostaining was performed on a Leica Biosystems BOND® RX instrument

  Counterstained with Hematoxylin.

  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins