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AB235352

Anti-SH3GLB2 antibody

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(1 Publication)

Rabbit Polyclonal SH3GLB2 antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human SH3GLB2 aa 1-400.

View Alternative Names

KIAA1848, PP578, SH3GLB2, Endophilin-B2, SH3 domain-containing GRB2-like protein B2

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SH3GLB2 antibody (AB235352)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SH3GLB2 antibody (AB235352)

Paraffin-embedded human placenta tissue stained for SPS2L/SPATS2L using ab235352 at 1/100 dilution in immunohistochemical analysis.

Western blot - Anti-SH3GLB2 antibody (AB235352)
  • WB

Supplier Data

Western blot - Anti-SH3GLB2 antibody (AB235352)

All lanes:

Western blot - Anti-SH3GLB2 antibody (ab235352) at 1/1000 dilution

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate

Lane 2:

Mouse gonadal tissue lysate

Lane 3:

Mouse lung tissue lysate

Lane 4:

U-87 MG (human glioblastoma-astrocytoma epithelial cell line) whole cell lysate

Lane 5:

THP-1 (human monocytic leukemia cell line) whole cell lysate

Lane 6:

A549 (human lung carcinoma cell line) whole cell lysate

Lane 7:

MCF7 (human breast adenocarcinoma cell line) whole cell lysate

Lane 8:

Mouse liver tissue lysate

Lane 9:

A431 (human epidermoid carcinoma cell line) whole cell lysate

Secondary

All lanes:

Goat polyclonal to Rabbit IgG at 1/10000 dilution

Predicted band size: 44 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human SH3GLB2 aa 1-400. The exact immunogen used to generate this antibody is proprietary information.

Q9NR46

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purity >95%
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SH3GLB2 also known as Bin1 or Amphiphysin II is a protein involved in membrane dynamics and intracellular trafficking. It has a molecular weight of approximately 40 kDa and is expressed in various tissues with significant expression in the brain heart and skeletal muscle. The protein contains a SH3 domain enabling it to interact with proline-rich motifs of partner proteins. This feature supports its role in processes involving membrane curvature and endocytosis.
Biological function summary

SH3GLB2 participates in the regulation of membrane remodeling. It acts as part of a larger complex that influences synaptic vesicle endocytosis by facilitating the scission of vesicles from the plasma membrane. This function is critical for maintaining cellular communication and signaling particularly in neuronal cells where rapid membrane trafficking is required. SH3GLB2's interactions allow for dynamic responses to cellular stress and help in maintaining cellular homeostasis.

Pathways

This protein is implicated in both the endocytic pathway and apoptotic processes. SH3GLB2 orchestrates interactions within the endocytic pathway alongside proteins such as clathrin and dynamin where it aids in vesicle formation and trafficking. Furthermore it contributes to the apoptotic pathway by modulating factors that influence cell survival and death interacting with proteins like Bax which plays a role in apoptosis regulation. These pathways highlight its involvement in maintaining cellular order and response to external stimuli.

Abnormalities in SH3GLB2 function have been linked to neurodegenerative diseases and certain cancers. In neurodegenerative diseases disrupted SH3GLB2 activity affects synaptic function and neuronal communication potentially contributing to conditions like Alzheimer's disease. Perturbations in SH3GLB2 interactions with proteins such as tau are noticeable in such disorders. In cancers altered expression or mutation of SH3GLB2 can influence cellular proliferation and invasion mainly through its impact on the endocytic and apoptotic pathways. This connection suggests its role as a potential biomarker or target for therapeutic interventions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information SH3GLB2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

The Journal of clinical endocrinology and metabolism 107:3328-3340 PubMed36074910

2022

Premature Ovarian Insufficiency in CLPB Deficiency: Transcriptomic, Proteomic and Phenotypic Insights.

Applications

Unspecified application

Species

Unspecified reactive species

Elena J Tucker,Megan J Baker,Daniella H Hock,Julia T Warren,Sylvie Jaillard,Katrina M Bell,Rajini Sreenivasan,Shabnam Bakhshalizadeh,Chloe A Hanna,Nikeisha J Caruana,Saskia B Wortmann,Shamima Rahman,Robert D S Pitceathly,Jean Donadieu,Aurelia Alimi,Vincent Launay,Paul Coppo,Sophie Christin-Maitre,Gorjana Robevska,Jocelyn van den Bergen,Brianna L Kline,Katie L Ayers,Phoebe N Stewart,David A Stroud,Diana Stojanovski,Andrew H Sinclair
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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