AB249013

Anti-SH3KBP1 antibody [EPR7881(2)] - BSA and Azide free

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal SH3KBP1 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

CIN85, SH3KBP1, SH3 domain-containing kinase-binding protein 1, CD2-binding protein 3, Cbl-interacting protein of 85 kDa, Human Src family kinase-binding protein 1, CD2BP3, HSB-1

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SH3KBP1 antibody [EPR7881(2)] - BSA and Azide free (AB249013)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SH3KBP1 antibody [EPR7881(2)] - BSA and Azide free (AB249013)

This data was developed using ab151574, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling SH3KBP1 with ab151574 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SH3KBP1 antibody [EPR7881(2)] - BSA and Azide free (AB249013)

This data was developed using ab151574, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human colonic adenocarcinoma tissue labeling SH3KBP1 with ab151574 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Unknown

Western blot - Anti-SH3KBP1 antibody [EPR7881(2)] - BSA and Azide free (AB249013)

This data was developed using ab151574, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-SH3KBP1 antibody [EPR7881(2)] (<a href='/en-us/products/primary-antibodies/sh3kbp1-antibody-epr78812-ab151574'>ab151574</a>) at 1/1000 dilution

Lane 1:

Jurkat cell lysate at 10 µg

Lane 2:

THP1 cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 73 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR7881(2)

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

ab249013 is the carrier-free version of ab151574.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The SH3 domain-containing kinase-binding protein 1 (SH3KBP1) also known as Cin85 is a multifunctional adapter protein involved in signal transduction pathways. It has a molecular mass of approximately 85 kDa. SH3KBP1 is expressed in a variety of tissues including the brain heart and lungs reflecting its diverse roles in cellular processes. This protein contains multiple SH3 domains that facilitate the binding with other proteins making SH3KBP1 an important modulator of protein-protein interactions.

Biological function summary

SH3KBP1 plays a role in cellular signaling and endocytosis. It forms part of a complex that participates in the regulation of membrane trafficking where it helps in internalization and down-regulation of receptor tyrosine kinases. SH3KBP1 interacts with other signaling molecules allowing it to coordinate the spatial and temporal dynamics of signaling pathways. This protein’s involvement in endocytic pathways highlights its significance in regulating cellular responses to external stimuli.

Pathways

SH3KBP1 is an integral part of the epidermal growth factor receptor (EGFR) signaling and the B-cell receptor (BCR) signaling pathways. In the EGFR pathway it facilitates receptor internalization affecting processes such as proliferation and survival. SH3KBP1 interacts with proteins like c-Cbl and Grb2 which are key players in receptor endocytosis and signal transduction. Through these interactions it modulates signal cascades that influence numerous cellular outcomes.

SH3KBP1 is implicated in cancer and autoimmune diseases. Its role in EGFR signaling connects it to cancer progression as dysregulation of this pathway can lead to uncontrolled cell growth. In autoimmune diseases SH3KBP1's interaction with BCR signaling components including CD19 and Syk can affect immune cell function and lead to pathological immune responses. Understanding the role of SH3KBP1 in these contexts can inform therapeutic strategies targeting its pathway involvement.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Adapter protein involved in regulating diverse signal transduction pathways. Involved in the regulation of endocytosis and lysosomal degradation of ligand-induced receptor tyrosine kinases, including EGFR and MET/hepatocyte growth factor receptor, through an association with CBL and endophilins. The association with CBL, and thus the receptor internalization, may be inhibited by an interaction with PDCD6IP and/or SPRY2. Involved in regulation of ligand-dependent endocytosis of the IgE receptor. Attenuates phosphatidylinositol 3-kinase activity by interaction with its regulatory subunit (By similarity). May be involved in regulation of cell adhesion; promotes the interaction between TTK2B and PDCD6IP. May be involved in the regulation of cellular stress response via the MAPK pathways through its interaction with MAP3K4. Is involved in modulation of tumor necrosis factor mediated apoptosis. Plays a role in the regulation of cell morphology and cytoskeletal organization. Required in the control of cell shape and migration. Has an essential role in the stimulation of B cell activation (PubMed : 29636373).

See full target information SH3KBP1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Autophagy 17:3361-3374 PubMed33404288

2021

Tumor heterogeneity in autophagy-dependent ferroptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Jingbo Li,Jiao Liu,Yinghua Xu,Runliu Wu,Xin Chen,Xinxin Song,Herbert Zeh,Rui Kang,Daniel J Klionsky,Xiaoyan Wang,Daolin Tang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SH3KBP1 antibody [EPR7881(2)] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SH3KBP1 antibody [EPR7881(2)] - BSA and Azide free (AB249013)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SH3KBP1 antibody [EPR7881(2)] - BSA and Azide free (AB249013)

Western blot - Anti-SH3KBP1 antibody [EPR7881(2)] - BSA and Azide free (AB249013)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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