AB250527

Anti-SH3PX1/SNX9 antibody [EPR14399] - BSA and Azide free

RabMAb
Recombinant
KO Validated
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(1 Publication)

Rabbit Recombinant Monoclonal SH3PX1/SNX9 antibody. Carrier free. Suitable for IHC-P, IP, WB, Flow Cyt (Intra), ICC/IF and reacts with Human, Mouse samples. Cited in 1 publication.

View Alternative Names

SH3PX1, SH3PXD3A, SNX9, Sorting nexin-9, SH3 and PX domain-containing protein 1, SH3 and PX domain-containing protein 3A, Protein SDP1

8 Images

Immunocytochemistry/ Immunofluorescence - Anti-SH3PX1/SNX9 antibody [EPR14399] - BSA and Azide free (AB250527)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SH3PX1/SNX9 antibody [EPR14399] - BSA and Azide free (AB250527)

This data was developed using ab181856, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of acetone-fixed HeLa cells labeling SH3PX1/SNX9 with ab181856 at 1/100 dilution, followed by Goat anti rabbit IgG (Alexa Fluor®488) secondary antibody at 1/200 dilution. Counter stained with Dapi.

Flow Cytometry (Intracellular) - Anti-SH3PX1/SNX9 antibody [EPR14399] - BSA and Azide free (AB250527)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-SH3PX1/SNX9 antibody [EPR14399] - BSA and Azide free (AB250527)

This data was developed using ab181856, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed HeLa cells labeling SH3PX1/SNX9 with ab181856 at 1/10 dilution (red) compared to a Rabbit IgG monoclonal isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SH3PX1/SNX9 antibody [EPR14399] - BSA and Azide free (AB250527)

This data was developed using ab181856, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% paraformaldehyde-fixed HCT-116 cells labeling SH3PX1/SNX9 with ab181856 at 1/100 dilution, followed by Goat anti rabbit IgG (Alexa Fluor®488) secondary antibody at 1/200 dilution. Counter stained with Dapi.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SH3PX1/SNX9 antibody [EPR14399] - BSA and Azide free (AB250527)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SH3PX1/SNX9 antibody [EPR14399] - BSA and Azide free (AB250527)

This data was developed using ab181856, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling SH3PX1/SNX9 with ab181856 at 1/250 dilution, followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

Supplier Data

Immunoprecipitation - Anti-SH3PX1/SNX9 antibody [EPR14399] - BSA and Azide free (AB250527)

This data was developed using ab181856, the same antibody clone in a different buffer formulation.

Western blot analysis of HepG2 cell lysate immunoprecipitated using ab181856 at 1/50 dilution. Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate secondary antibody was used at 1/1000 dilution.

All lanes:

Immunoprecipitation - Anti-SH3PX1/SNX9 antibody [EPR14399] (<a href='/en-us/products/primary-antibodies/sh3px1-snx9-antibody-epr14399-ab181856'>ab181856</a>)

Predicted band size: 67 kDa

false

Lab

Western blot - Anti-SH3PX1/SNX9 antibody [EPR14399] - BSA and Azide free (AB250527)

This data was developed using ab181856, the same antibody clone in a different buffer formulation.

Lane 1 : Wild-type HAP1 cell lysate (20 μg)

Lane 2 : SH3PX1/SNX9 knockout HAP1 cell lysate (20 μg)

Lane 3 : HeLa cell lysate (20 μg)

Lane 4 : HCT116 cell lysate (20 μg)

Lanes 1 - 4 : Merged signal (red and green). Green - ab181856 observed at 75 kDa. Red - loading control, ab8245, observed at 37 kDa.

ab181856 was shown to specifically react with SH3PX1/SNX9 when SH3PX1/SNX9 knockout samples were used. Wild-type and SH3PX1/SNX9 knockout samples were subjected to SDS-PAGE. ab181856 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-SH3PX1/SNX9 antibody [EPR14399] (<a href='/en-us/products/primary-antibodies/sh3px1-snx9-antibody-epr14399-ab181856'>ab181856</a>)

Predicted band size: 67 kDa

false

Supplier Data

Western blot - Anti-SH3PX1/SNX9 antibody [EPR14399] - BSA and Azide free (AB250527)

This data was developed using ab181856, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-SH3PX1/SNX9 antibody [EPR14399] (<a href='/en-us/products/primary-antibodies/sh3px1-snx9-antibody-epr14399-ab181856'>ab181856</a>) at 1/50000 dilution

All lanes:

HeLa cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 67 kDa

Observed band size: 78 kDa

false

Supplier Data

Western blot - Anti-SH3PX1/SNX9 antibody [EPR14399] - BSA and Azide free (AB250527)

This data was developed using ab181856, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-SH3PX1/SNX9 antibody [EPR14399] (<a href='/en-us/products/primary-antibodies/sh3px1-snx9-antibody-epr14399-ab181856'>ab181856</a>) at 1/10000 dilution

All lanes:

HCT-116 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 67 kDa

Observed band size: 78 kDa

false

Unconjugated

Anti-SH3PX1/SNX9 antibody [EPR14399]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14399

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Human

Applications

ICC/IF, IHC-P, Flow Cyt (Intra), IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

ab250527 is the carrier-free version of ab181856.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SH3PX1 also known as SNX9 is a protein with a mass of approximately 69 kDa. This protein contains an SH3 domain which enables it to bind to other proteins and participate in intracellular signaling pathways. SNX9 is widely expressed in various tissues including the brain and liver indicating a broad functional role in cellular processes. The expression of SNX9 in different cell types suggests its involvement in multiple cellular processes including membrane trafficking and cytoskeletal dynamics.

Biological function summary

Functions of SNX9 extend to cellular endocytosis and membrane remodeling. It is part of the retromer complex which plays a role in sorting endosomal proteins back to the trans-Golgi network or plasma membrane. SNX9 facilitates the curvature of cellular membranes aiding the process of clathrin-mediated endocytosis. This protein's ability to bind with both phospholipids and cytoskeletal components highlights its importance in maintaining cellular architecture and facilitating signal transduction.

Pathways

SNX9 integrates into the endocytic and signaling pathways. Its involvement in clathrin-mediated endocytosis and interactions with proteins like dynamin and WASP indicate its key role in vesicle formation and intracellular transport. SNX9 also participates within the Wnt signaling pathway functioning alongside proteins like LRP6 and GSK3 to mediate signal transduction processes that regulate cell fate determination. These pathways underline SNX9’s significance in processes controlling cellular growth and differentiation.

Alterations in SNX9 expression or function have associations with neurological disorders and cancer. SNX9 interactions with proteins like Cdc42 and cortactin highlight its relevance in brain development and its implications in schizophrenia. Abnormalities in SNX9-mediated endocytosis can disrupt cellular signaling leading to oncogenic transformation and contributing to the progression of cancers such as breast cancer. Understanding SNX9's role in these conditions may offer insights into potential therapeutic targets.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Involved in endocytosis and intracellular vesicle trafficking, both during interphase and at the end of mitosis. Required for efficient progress through mitosis and cytokinesis. Required for normal formation of the cleavage furrow at the end of mitosis. Plays a role in endocytosis via clathrin-coated pits, but also clathrin-independent, actin-dependent fluid-phase endocytosis. Plays a role in macropinocytosis. Promotes internalization of TNFR. Promotes degradation of EGFR after EGF signaling. Stimulates the GTPase activity of DNM1. Promotes DNM1 oligomerization. Promotes activation of the Arp2/3 complex by WASL, and thereby plays a role in the reorganization of the F-actin cytoskeleton. Binds to membranes enriched in phosphatidylinositol 4,5-bisphosphate and promotes membrane tubulation. Has lower affinity for membranes enriched in phosphatidylinositol 3-phosphate.

See full target information SNX9

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 14:15564 PubMed38971897

2024

LILRB4 knockdown inhibits aortic dissection development by regulating pyroptosis and the JAK2/STAT3 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jianxian Xiong,Jiayuan Ling,Jie Yan,Yanyu Duan,Junjian Yu,Wentong Li,Wenbo Yu,Jianfeng Gao,Dilin Xie,Ziyou Liu,Yongzhi Deng,Yongling Liao

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SH3PX1/SNX9 antibody [EPR14399] - BSA and Azide free

Immunocytochemistry/ Immunofluorescence - Anti-SH3PX1/SNX9 antibody [EPR14399] - BSA and Azide free (AB250527)

Flow Cytometry (Intracellular) - Anti-SH3PX1/SNX9 antibody [EPR14399] - BSA and Azide free (AB250527)

Immunocytochemistry/ Immunofluorescence - Anti-SH3PX1/SNX9 antibody [EPR14399] - BSA and Azide free (AB250527)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SH3PX1/SNX9 antibody [EPR14399] - BSA and Azide free (AB250527)

Immunoprecipitation - Anti-SH3PX1/SNX9 antibody [EPR14399] - BSA and Azide free (AB250527)

Western blot - Anti-SH3PX1/SNX9 antibody [EPR14399] - BSA and Azide free (AB250527)

Western blot - Anti-SH3PX1/SNX9 antibody [EPR14399] - BSA and Azide free (AB250527)

Western blot - Anti-SH3PX1/SNX9 antibody [EPR14399] - BSA and Azide free (AB250527)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise