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Rabbit Recombinant Monoclonal SH3PX1/SNX9 antibody. Carrier free. Suitable for IHC-P, IP, WB, Flow Cyt (Intra), ICC/IF and reacts with Human, Mouse samples.

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Images

Immunoprecipitation - Anti-SH3PX1/SNX9 antibody [EPR14399] - BSA and Azide free (AB250527), expandable thumbnail
  • Western blot - Anti-SH3PX1/SNX9 antibody [EPR14399] - BSA and Azide free (AB250527), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-SH3PX1/SNX9 antibody [EPR14399] - BSA and Azide free (AB250527), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-SH3PX1/SNX9 antibody [EPR14399] - BSA and Azide free (AB250527), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-SH3PX1/SNX9 antibody [EPR14399] - BSA and Azide free (AB250527), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PIPWBFlow Cyt (Intra)ICC/IF
Human
Tested
Tested
Tested
Tested
Tested
Mouse
Expected
Expected
Expected
Expected
Expected
Rat
Predicted
Predicted
Predicted
Predicted
Predicted

Tested
Tested

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species
Mouse
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Predicted
Predicted

Species
Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species
Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Rat
Dilution info
-
Notes

-

Target data

Function

Involved in endocytosis and intracellular vesicle trafficking, both during interphase and at the end of mitosis. Required for efficient progress through mitosis and cytokinesis. Required for normal formation of the cleavage furrow at the end of mitosis. Plays a role in endocytosis via clathrin-coated pits, but also clathrin-independent, actin-dependent fluid-phase endocytosis. Plays a role in macropinocytosis. Promotes internalization of TNFR. Promotes degradation of EGFR after EGF signaling. Stimulates the GTPase activity of DNM1. Promotes DNM1 oligomerization. Promotes activation of the Arp2/3 complex by WASL, and thereby plays a role in the reorganization of the F-actin cytoskeleton. Binds to membranes enriched in phosphatidylinositol 4,5-bisphosphate and promotes membrane tubulation. Has lower affinity for membranes enriched in phosphatidylinositol 3-phosphate.

Alternative names

SH3PX1, SH3PXD3A, SNX9, Sorting nexin-9, SH3 and PX domain-containing protein 1, SH3 and PX domain-containing protein 3A, Protein SDP1

Recommended products

Rabbit Recombinant Monoclonal SH3PX1/SNX9 antibody. Carrier free. Suitable for IHC-P, IP, WB, Flow Cyt (Intra), ICC/IF and reacts with Human, Mouse samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR14399
Purification technique
Affinity purification
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab250527 is the carrier-free version of Anti-SH3PX1/SNX9 antibody [EPR14399] ab181856.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

SH3PX1 also known as SNX9 is a protein with a mass of approximately 69 kDa. This protein contains an SH3 domain which enables it to bind to other proteins and participate in intracellular signaling pathways. SNX9 is widely expressed in various tissues including the brain and liver indicating a broad functional role in cellular processes. The expression of SNX9 in different cell types suggests its involvement in multiple cellular processes including membrane trafficking and cytoskeletal dynamics.

Biological function summary

Functions of SNX9 extend to cellular endocytosis and membrane remodeling. It is part of the retromer complex which plays a role in sorting endosomal proteins back to the trans-Golgi network or plasma membrane. SNX9 facilitates the curvature of cellular membranes aiding the process of clathrin-mediated endocytosis. This protein's ability to bind with both phospholipids and cytoskeletal components highlights its importance in maintaining cellular architecture and facilitating signal transduction.

Pathways

SNX9 integrates into the endocytic and signaling pathways. Its involvement in clathrin-mediated endocytosis and interactions with proteins like dynamin and WASP indicate its key role in vesicle formation and intracellular transport. SNX9 also participates within the Wnt signaling pathway functioning alongside proteins like LRP6 and GSK3 to mediate signal transduction processes that regulate cell fate determination. These pathways underline SNX9’s significance in processes controlling cellular growth and differentiation.

Associated diseases and disorders

Alterations in SNX9 expression or function have associations with neurological disorders and cancer. SNX9 interactions with proteins like Cdc42 and cortactin highlight its relevance in brain development and its implications in schizophrenia. Abnormalities in SNX9-mediated endocytosis can disrupt cellular signaling leading to oncogenic transformation and contributing to the progression of cancers such as breast cancer. Understanding SNX9's role in these conditions may offer insights into potential therapeutic targets.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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