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AB154123

Anti-SH3YL1 antibody

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(2 Publications)

Rabbit Polyclonal SH3YL1 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SH3YL1 aa 50-300.

View Alternative Names

SH3 domain-containing YSC84-like protein 1, SH3YL1

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SH3YL1 antibody (AB154123)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SH3YL1 antibody (AB154123)

Immunohistochemical analysis of paraffin embedded U87 xenograft tissue labeling SH3YL1 with ab154123 at 1/500.

Western blot - Anti-SH3YL1 antibody (AB154123)
  • WB

Unknown

Western blot - Anti-SH3YL1 antibody (AB154123)

10% SDS PAGE

All lanes:

Western blot - Anti-SH3YL1 antibody (ab154123) at 1/1000 dilution

Lane 1:

NT2D1 whole cell lysate at 30 µg

Lane 2:

PC3 whole cell lysate at 30 µg

Lane 3:

U87 MG whole cell lysate at 30 µg

Lane 4:

SK N SH whole cell lysate at 30 µg

Predicted band size: 37 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human SH3YL1 aa 50-300. The exact immunogen used to generate this antibody is proprietary information.

Q96HL8

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SH3YL1 also known as SH3 domain containing Ysc84-like protein 1 is a protein with a molecular mass of around 42 kDa. Mechanically SH3YL1 acts as a cytoplasmic adapter protein engaging in protein-protein interactions through its SH3 domain which is a common protein interaction domain found in many proteins involved in signal transduction. SH3YL1 expression occurs mainly in neural tissues but it can be found in other tissues at varying levels.
Biological function summary

SH3YL1 participates in intracellular signaling processes that are essential for cell shape and movement. While not part of a large multi-protein complex it interacts with proteins involved in actin cytoskeletal dynamics. Its role in modulating the actin cytoskeleton influences processes like cell adhesion and migration making it an important player in developmental biology and tissue organization.

Pathways

SH3YL1 integrates into signaling pathways that regulate actin cytoskeleton rearrangements such as the Rac1-WAVE2 signaling pathway. In this pathway SH3YL1 interacts with WAVE2 a member of the WAVE regulatory complex to promote actin polymerization. These interactions highlight its relevance in cellular processes such as endocytosis and membrane trafficking linking it to other signaling molecules like Rac1 which further modulates the actin cytoskeleton through related pathways.

SH3YL1 exhibits connections to disorders involving aberrant cell migration and invasion such as cancer metastasis. Increased SH3YL1 activity correlates with enhanced invasive potential in certain cancer cells which may involve its interaction with proteins like WAVE2 in altering cytoskeletal dynamics. Further studies could show a clearer role in neurodegenerative diseases where abnormal cytoskeleton regulation plays a critical role in disease progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information SH3YL1
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of cellular physiology 238:1063-1079 PubMed36924084

2023

Identification of circular dorsal ruffles as signal platforms for the AKT pathway in glomerular podocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Rui Hua,Jinzi Wei,Mauricio Torres,Yuxin He,Yanan Li,Xiaowei Sun,Li Wang,Ken Inoki,Sei Yoshida

Journal of cellular physiology 234:927-939 PubMed30144066

2018

Downregulated microRNA-340-5p promotes proliferation and inhibits apoptosis of chondrocytes in osteoarthritis mice through inhibiting the extracellular signal-regulated kinase signaling pathway by negatively targeting the FMOD gene.

Applications

Unspecified application

Species

Unspecified reactive species

Weikai Zhang,Peng Cheng,Weihua Hu,Weifeng Yin,Fengjing Guo,Anmin Chen,Hui Huang
View all publications
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