AB154224

Anti-SHANK1 antibody

Name: Anti-SHANK1 antibody (ab154224) | Abcam
Brand: Abcam Limited
SKU: AB154224
Price: 535 USD
Availability: InStock

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(1 Publication)

Rabbit Polyclonal SHANK1 antibody. Suitable for IHC-Fr, IHC-P, WB and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human SHANK1 aa 2100 to C-terminus.

View Alternative Names

SH3 and multiple ankyrin repeat domains protein 1, Shank1, Somatostatin receptor-interacting protein, SSTR-interacting protein, SSTRIP, SHANK1

7 Images

Immunocytochemistry/ Immunofluorescence - Anti-SHANK1 antibody (AB154224)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SHANK1 antibody (AB154224)

Immunofluorescence analysis of SKNSH cells fixed in 4% paraformaldehyde at RT for 15 min labelling SHANK1 protein at cytoplasm using ab154224 at a 1/500 dilution (Green).
Hoechst 33342 was used for nuclear staining (Blue).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHANK1 antibody (AB154224)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHANK1 antibody (AB154224)

Immunohistochemical analysis of paraffin embedded Human J5 xenograft tissue labelling SW480 with ab154224 at a 1/1000 dilution.

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SHANK1 antibody (AB154224)

Immunofluorescence analysis of DIV9 rat E18 primary cortical neurons fixed in 4% paraformaldehyde at RT for 15 min labelling SHANK1 protein at cell body and synaptic vesicles using ab154224 at a 1/500 dilution (Green).
beta Tubulin 3/ Tuj1, a neuron cell marker, stained by beta Tubulin 3/ Tuj1 antibody at a 1/500 dilution (Red). DAPI was used for nuclear staining (Blue).

Immunohistochemistry (Frozen sections) - Anti-SHANK1 antibody (AB154224)

IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-SHANK1 antibody (AB154224)

Immunohistochemical analysis of Frozen sectioned adult mouse cerebellum labeling SHANK1 protein expression with ab154224 at 1/250 dilution (Green). beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody diluted at 1/500.
Blue : Fluoroshield with DAPI .

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHANK1 antibody (AB154224)

Immunohistochemical analysis of paraffin-embedded mouse retina tissue stained for SHANK1 protein with ab154224 at a 1/250 dilution.

Antigen retrieval : Citrate buffer, pH 6.0, 15 min.

Supplier Data

Western blot - Anti-SHANK1 antibody (AB154224)

Samples were separated by 5% SDS-PAGE.

All lanes:

Western blot - Anti-SHANK1 antibody (ab154224) at 1/500 dilution

Lane 1:

U87-MG whole cell extracts at 30 µg

Lane 2:

SK-N-SH whole cell extracts at 30 µg

Lane 3:

IMR32 whole cell extracts at 30 µg

Lane 4:

SK-N-AS whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 225 kDa

false

Supplier Data

Western blot - Anti-SHANK1 antibody (AB154224)

Samples were separated by 5% SDS-PAGE.

All lanes:

Western blot - Anti-SHANK1 antibody (ab154224) at 1/1000 dilution

Lane 1:

Boiled mouse brain tissue extracts at 50 µg

Lane 2:

Boiled mouse liver tissue extracts at 50 µg

Lane 3:

Unboiled mouse brain tissue extracts at 50 µg

Lane 4:

Unboiled mouse liver tissue extracts at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 25 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, IHC-P, IHC-Fr

applications

Immunogen

Recombinant Fragment Protein within Human SHANK1 aa 2100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9Y566

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/100 - 1/1000", "IHCFr-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "" }, "Mouse": { "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "" }, "Rat": { "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SHANK1 also known as SH3 and multiple ankyrin repeat domains protein 1 plays an important role in the structural organization of synapses. It is a scaffold protein found in postsynaptic density where it interacts with other molecules to maintain synaptic architecture. The protein has a molecular mass of approximately 240 kDa. Expression of SHANK1 is most abundant in the brain especially in the hippocampus and cortex where it is essential for establishing and stabilizing synaptic connections during neuronal development.

Biological function summary

SHANK1 participates in the formation of macromolecular complexes that are critical for synaptic signaling and plasticity. It forms complexes with other postsynaptic proteins including PSD-95 and Homer which together coordinate the organization of synaptic receptors and signaling molecules. This interaction determines synaptic strength and plays a role in the modulation of synaptic transmission influencing the communication between neurons.

Pathways

SHANK1 is involved in the glutamatergic synaptic transmission pathway which is important for neural communication and cognitive functions such as learning and memory. Through this pathway SHANK1 interacts with NMDA receptors and mGluR receptors facilitating the propagation of excitatory signals. It also connects with proteins like SAPAP and cortactin which help stabilize the synaptic complex influencing cytoskeletal dynamics and synaptic plasticity.

SHANK1 has been implicated in autism spectrum disorders and schizophrenia where disrupted synaptic signaling plays an important role. Mutations in SHANK1 or its regulatory interactions can lead to altered neuronal connectivity and synaptic function which contribute to the pathology of these conditions. The protein's interaction with SHANK3 and other synaptic proteins like neuroligin emphasizes its role in synaptic disorders as these partners also participate in maintaining synaptic structure and function.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Seems to be an adapter protein in the postsynaptic density (PSD) of excitatory synapses that interconnects receptors of the postsynaptic membrane including NMDA-type and metabotropic glutamate receptors via complexes with GKAP/PSD-95 and Homer, respectively, and the actin-based cytoskeleton. Plays a role in the structural and functional organization of the dendritic spine and synaptic junction.

See full target information SHANK1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 13:403 PubMed35468874

2022

SHANK1 facilitates non-small cell lung cancer processes through modulating the ubiquitination of Klotho by interacting with MDM2.

Applications

Unspecified application

Species

Unspecified reactive species

Bo Chen,Hongye Zhao,Min Li,Quan She,Wen Liu,Jiayi Zhang,Weihong Zhao,Shuhong Huang,Jianqing Wu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SHANK1 antibody

Immunocytochemistry/ Immunofluorescence - Anti-SHANK1 antibody (AB154224)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHANK1 antibody (AB154224)

Immunocytochemistry/ Immunofluorescence - Anti-SHANK1 antibody (AB154224)

Immunohistochemistry (Frozen sections) - Anti-SHANK1 antibody (AB154224)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHANK1 antibody (AB154224)

Western blot - Anti-SHANK1 antibody (AB154224)

Western blot - Anti-SHANK1 antibody (AB154224)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise