Rabbit Recombinant Monoclonal SHANK1 antibody. Suitable for WB, IHC-P and reacts with Mouse, Rat samples.
IgG
Rabbit
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Monoclonal
WB | IHC-P | |
---|---|---|
Mouse | Tested | Expected |
Rat | Tested | Tested |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/1000 | Notes - |
Species Rat | Dilution info 1/1000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat | Dilution info 0.5-1 µg/mL | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Select an associated product type
Seems to be an adapter protein in the postsynaptic density (PSD) of excitatory synapses that interconnects receptors of the postsynaptic membrane including NMDA-type and metabotropic glutamate receptors, and the actin-based cytoskeleton. Plays a role in the structural and functional organization of the dendritic spine and synaptic junction. Overexpression promotes maturation of dendritic spines and the enlargement of spine heads via its ability to recruit Homer to postsynaptic sites, and enhances presynaptic function (By similarity).
SH3 and multiple ankyrin repeat domains protein 1, Shank1, Shank1
Rabbit Recombinant Monoclonal SHANK1 antibody. Suitable for WB, IHC-P and reacts with Mouse, Rat samples.
IgG
Rabbit
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Monoclonal
R25143-84
Affinity purification Protein A
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
This supplementary information is collated from multiple sources and compiled automatically.
SHANK1 also known as SH3 and multiple ankyrin repeat domains protein 1 plays an important role in the structural organization of synapses. It is a scaffold protein found in postsynaptic density where it interacts with other molecules to maintain synaptic architecture. The protein has a molecular mass of approximately 240 kDa. Expression of SHANK1 is most abundant in the brain especially in the hippocampus and cortex where it is essential for establishing and stabilizing synaptic connections during neuronal development.
SHANK1 participates in the formation of macromolecular complexes that are critical for synaptic signaling and plasticity. It forms complexes with other postsynaptic proteins including PSD-95 and Homer which together coordinate the organization of synaptic receptors and signaling molecules. This interaction determines synaptic strength and plays a role in the modulation of synaptic transmission influencing the communication between neurons.
SHANK1 is involved in the glutamatergic synaptic transmission pathway which is important for neural communication and cognitive functions such as learning and memory. Through this pathway SHANK1 interacts with NMDA receptors and mGluR receptors facilitating the propagation of excitatory signals. It also connects with proteins like SAPAP and cortactin which help stabilize the synaptic complex influencing cytoskeletal dynamics and synaptic plasticity.
SHANK1 has been implicated in autism spectrum disorders and schizophrenia where disrupted synaptic signaling plays an important role. Mutations in SHANK1 or its regulatory interactions can lead to altered neuronal connectivity and synaptic function which contribute to the pathology of these conditions. The protein's interaction with SHANK3 and other synaptic proteins like neuroligin emphasizes its role in synaptic disorders as these partners also participate in maintaining synaptic structure and function.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
IHC image of SHANK1 staining in a section of formalin-fixed paraffin-embedded normal rat cerebellum. Performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with EDTA buffer (pH9.0) at 100°C for 20 minutes. The section was then incubated with ab313127 (at 0.5 µg/ml) for 15 mins at room temperature and detected using an HRP conjugated compact polymer.
Negative control: Secondary antibody only.
Hematoxylin was used as a counterstain. For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.
ab313127 was shown to specifically react with SHANK1 in mouse and rat brain tissue lysates. Samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. ab313127 and Anti-alpha Tubulin antibody [DM1A] - Loading Control were incubated overnight at 4°C at 1/1000 and 1 /20000 dilutions, respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-SHANK1 antibody [EPR25143-84] (ab313127) at 1/1000 dilution
Lane 1: Mouse brain tissue lysate at 20 µg
Lane 2: Rat brain tissue lysate at 20 µg
Lane 3: Mouse lung tissue lysate at 20 µg
Lane 4: Rat lung tissue lysate at 20 µg
Lanes 1 - 4: Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed at 1/20000 dilution
Lanes 1 - 4: Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed at 1/20000 dilution
Performed under reducing conditions.
Observed band size: 253 kDa, 50 kDa
IHC image of SHANK1 staining in a section of formalin-fixed paraffin-embedded normal rat heart. Performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with EDTA buffer (pH9.0) at 100°C for 20 minutes. The section was then incubated with ab313127 (at 1 µg/ml) for 15 mins at room temperature and detected using an HRP conjugated compact polymer.
Negative control: Secondary antibody only.
Hematoxylin was used as a counterstain. For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com