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Rabbit Recombinant Monoclonal SHANK1 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Mouse, Rat samples.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHANK1 antibody [EPR25143-84] - BSA and Azide free (AB287994), expandable thumbnail
  • Western blot - Anti-SHANK1 antibody [EPR25143-84] - BSA and Azide free (AB287994), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHANK1 antibody [EPR25143-84] - BSA and Azide free (AB287994), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-P
Mouse
Tested
Expected
Rat
Tested
Tested

Tested
Tested

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Rat
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species
Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Version

Target data

Function

Seems to be an adapter protein in the postsynaptic density (PSD) of excitatory synapses that interconnects receptors of the postsynaptic membrane including NMDA-type and metabotropic glutamate receptors, and the actin-based cytoskeleton. Plays a role in the structural and functional organization of the dendritic spine and synaptic junction. Overexpression promotes maturation of dendritic spines and the enlargement of spine heads via its ability to recruit Homer to postsynaptic sites, and enhances presynaptic function (By similarity).

Alternative names

SH3 and multiple ankyrin repeat domains protein 1, Shank1

Recommended products

Rabbit Recombinant Monoclonal SHANK1 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Mouse, Rat samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
R25143-84
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Notes

ab287994 is the carrirer-free version of Anti-SHANK1 antibody [EPR25143-84] ab313127.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

SHANK1 also known as SH3 and multiple ankyrin repeat domains protein 1 plays an important role in the structural organization of synapses. It is a scaffold protein found in postsynaptic density where it interacts with other molecules to maintain synaptic architecture. The protein has a molecular mass of approximately 240 kDa. Expression of SHANK1 is most abundant in the brain especially in the hippocampus and cortex where it is essential for establishing and stabilizing synaptic connections during neuronal development.

Biological function summary

SHANK1 participates in the formation of macromolecular complexes that are critical for synaptic signaling and plasticity. It forms complexes with other postsynaptic proteins including PSD-95 and Homer which together coordinate the organization of synaptic receptors and signaling molecules. This interaction determines synaptic strength and plays a role in the modulation of synaptic transmission influencing the communication between neurons.

Pathways

SHANK1 is involved in the glutamatergic synaptic transmission pathway which is important for neural communication and cognitive functions such as learning and memory. Through this pathway SHANK1 interacts with NMDA receptors and mGluR receptors facilitating the propagation of excitatory signals. It also connects with proteins like SAPAP and cortactin which help stabilize the synaptic complex influencing cytoskeletal dynamics and synaptic plasticity.

Associated diseases and disorders

SHANK1 has been implicated in autism spectrum disorders and schizophrenia where disrupted synaptic signaling plays an important role. Mutations in SHANK1 or its regulatory interactions can lead to altered neuronal connectivity and synaptic function which contribute to the pathology of these conditions. The protein's interaction with SHANK3 and other synaptic proteins like neuroligin emphasizes its role in synaptic disorders as these partners also participate in maintaining synaptic structure and function.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHANK1 antibody [EPR25143-84] - BSA and Azide free (ab287994), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHANK1 antibody [EPR25143-84] - BSA and Azide free (ab287994)

    This data was developed using Anti-SHANK1 antibody [EPR25143-84] ab313127, the same antibody clone in a different buffer formulation.

    IHC image of SHANK1 staining in a section of formalin-fixed paraffin-embedded normal rat cerebellum. Performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with EDTA buffer (pH9.0) at 100°C for 20 minutes. The section was then incubated with Anti-SHANK1 antibody [EPR25143-84] ab313127 (at 0.5 µg/ml) for 15 mins at room temperature and detected using an HRP conjugated compact polymer.

    Negative control: Secondary antibody only.

    Hematoxylin was used as a counterstain. For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.

  • Western blot - Anti-SHANK1 antibody [EPR25143-84] - BSA and Azide free (ab287994), expandable thumbnail

    Western blot - Anti-SHANK1 antibody [EPR25143-84] - BSA and Azide free (ab287994)

    This data was developed using Anti-SHANK1 antibody [EPR25143-84] ab313127, the same antibody clone in a different buffer formulation.

    Anti-SHANK1 antibody [EPR25143-84] ab313127 was shown to specifically react with SHANK1 in mouse and rat brain tissue lysates. Samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Anti-SHANK1 antibody [EPR25143-84] ab313127 and Anti-alpha Tubulin antibody [DM1A] - Loading Control were incubated overnight at 4°C at 1/1000 and 1 /20000 dilutions, respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-SHANK1 antibody [EPR25143-84] (Anti-SHANK1 antibody [EPR25143-84] ab313127) at 1/1000 dilution

    Lane 1: Mouse brain tissue lysate at 20 µg with 3% milk in TBS-0.1 % Tween® 20 (TBS-T)

    Lane 2: Rat brain tissue lysate at 20 µg with 3% milk in TBS-0.1 % Tween® 20 (TBS-T)

    Lane 3: Mouse lung tissue lysate at 20 µg with 3% milk in TBS-0.1 % Tween® 20 (TBS-T)

    Lane 4: Rat lung tissue lysate at 20 µg with 3% milk in TBS-0.1 % Tween® 20 (TBS-T)

    Secondary

    Lanes 1 - 4: Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed at 1/20000 dilution

    Lanes 1 - 4: Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed at 1/20000 dilution

    Performed under reducing conditions.

    Observed band size: 253 kDa, 50 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHANK1 antibody [EPR25143-84] - BSA and Azide free (ab287994), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHANK1 antibody [EPR25143-84] - BSA and Azide free (ab287994)

    This data was developed using Anti-SHANK1 antibody [EPR25143-84] ab313127, the same antibody clone in a different buffer formulation.

    IHC image of SHANK1 staining in a section of formalin-fixed paraffin-embedded normal rat heart. Performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with EDTA buffer (pH9.0) at 100°C for 20 minutes. The section was then incubated with Anti-SHANK1 antibody [EPR25143-84] ab313127 (at 1 µg/ml) for 15 mins at room temperature and detected using an HRP conjugated compact polymer.

    Negative control: Secondary antibody only.

    Hematoxylin was used as a counterstain. For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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