Anti-SHANK1 antibody [EPR25143-84] - BSA and Azide free

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHANK1 antibody [EPR25143-84] - BSA and Azide free (AB287994)

This data was developed using ab313127, the same antibody clone in a different buffer formulation.

IHC image of SHANK1 staining in a section of formalin-fixed paraffin-embedded normal rat heart. Performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with EDTA buffer (pH9.0) at 100°C for 20 minutes. The section was then incubated with ab313127 (at 1 µg/ml) for 15 mins at room temperature and detected using an HRP conjugated compact polymer.

Negative control : Secondary antibody only.

Hematoxylin was used as a counterstain. For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHANK1 antibody [EPR25143-84] - BSA and Azide free (AB287994)

This data was developed using ab313127, the same antibody clone in a different buffer formulation.

IHC image of SHANK1 staining in a section of formalin-fixed paraffin-embedded normal rat cerebellum. Performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with EDTA buffer (pH9.0) at 100°C for 20 minutes. The section was then incubated with ab313127 (at 0.5 µg/ml) for 15 mins at room temperature and detected using an HRP conjugated compact polymer.

Negative control : Secondary antibody only.

Hematoxylin was used as a counterstain. For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.

• WB

Lab

Western blot - Anti-SHANK1 antibody [EPR25143-84] - BSA and Azide free (AB287994)

This data was developed using ab313127, the same antibody clone in a different buffer formulation.

ab313127 was shown to specifically react with SHANK1 in mouse and rat brain tissue lysates. Samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. ab313127 and Anti-alpha Tubulin antibody [DM1A] - Loading Control were incubated overnight at 4°C at 1/1000 and 1 /20000 dilutions, respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-SHANK1 antibody [EPR25143-84] (<a href='/en-us/products/primary-antibodies/shank1-antibody-epr25143-84-ab313127'>ab313127</a>) at 1/1000 dilution

Lane 1:

Mouse brain tissue lysate at 20 µg

Lane 2:

Rat brain tissue lysate at 20 µg

Lane 3:

Mouse lung tissue lysate at 20 µg

Lane 4:

Rat lung tissue lysate at 20 µg

Secondary

Lanes 1 - 4:

Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed at 1/20000 dilution

Lanes 1 - 4:

Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed at 1/20000 dilution

Observed band size: 253 kDa,50 kDa

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