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Mouse Monoclonal SHANK1 antibody. Suitable for ICC/IF, Flow Cyt, WB, IHC-P and reacts with Human, Rat, Mouse samples. Cited in 3 publications. Immunogen corresponding to Recombinant Protein within Rat Shank1 aa 450-700.

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Images

Western blot - Anti-SHANK1 antibody [N22/21] (AB94576), expandable thumbnail
  • Flow Cytometry - Anti-SHANK1 antibody [N22/21] (AB94576), expandable thumbnail
  • Immunohistochemistry paraffin embedded sections - Anti-SHANK1 antibody [N22/21] (AB94576), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-SHANK1 antibody [N22/21] (AB94576), expandable thumbnail

Publications

Key facts

Isotype

IgG1

Host species

Mouse

Storage buffer

Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Form

Liquid

Clonality

Monoclonal

Immunogen

  • Recombinant Protein within Rat Shank1 aa 450-700. The exact immunogen used to generate this antibody is proprietary information. Database link Q9WV48

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFFlow CytWBIHC-P
Human
Tested
Tested
Expected
Expected
Mouse
Expected
Expected
Expected
Tested
Rat
Expected
Expected
Tested
Expected

Tested
Tested

Species

Human

Dilution info

1/50

Notes

-

Expected
Expected

Species

Rat, Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Human

Dilution info

1 µg for 106 Cells

Notes

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Expected
Expected

Species

Rat, Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Rat

Dilution info

1-10 µg/mL

Notes

-

Expected
Expected

Species

Human, Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Mouse

Dilution info

-

Notes

-

Expected
Expected

Species

Human, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Associated Products

Select an associated product type

2 products for Alternative Product

Target data

Function

Seems to be an adapter protein in the postsynaptic density (PSD) of excitatory synapses that interconnects receptors of the postsynaptic membrane including NMDA-type and metabotropic glutamate receptors via complexes with GKAP/PSD-95 and Homer, respectively, and the actin-based cytoskeleton. Plays a role in the structural and functional organization of the dendritic spine and synaptic junction.

Alternative names

SH3 and multiple ankyrin repeat domains protein 1, Shank1, Somatostatin receptor-interacting protein, SSTR-interacting protein, SSTRIP, SHANK1

Recommended products

Mouse Monoclonal SHANK1 antibody. Suitable for ICC/IF, Flow Cyt, WB, IHC-P and reacts with Human, Rat, Mouse samples. Cited in 3 publications. Immunogen corresponding to Recombinant Protein within Rat Shank1 aa 450-700.

Alternative names

SH3 and multiple ankyrin repeat domains protein 1, Shank1, Somatostatin receptor-interacting protein, SSTR-interacting protein, SSTRIP, SHANK1

Key facts

Isotype

IgG1

Form

Liquid

Clonality

Monoclonal

Immunogen
  • Recombinant Protein within Rat Shank1 aa 450-700. The exact immunogen used to generate this antibody is proprietary information. Database link Q9WV48
Clone number

N22/21

Purification technique

Affinity purification Protein G

Specificity

ab94576 does not react with SHANK2 and SHANK3

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

The clone number has been updated from S22-21 to N22/21, both clone numbers name the same antibody clone.

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Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

SHANK1 also known as SH3 and multiple ankyrin repeat domains protein 1 plays an important role in the structural organization of synapses. It is a scaffold protein found in postsynaptic density where it interacts with other molecules to maintain synaptic architecture. The protein has a molecular mass of approximately 240 kDa. Expression of SHANK1 is most abundant in the brain especially in the hippocampus and cortex where it is essential for establishing and stabilizing synaptic connections during neuronal development.

Biological function summary

SHANK1 participates in the formation of macromolecular complexes that are critical for synaptic signaling and plasticity. It forms complexes with other postsynaptic proteins including PSD-95 and Homer which together coordinate the organization of synaptic receptors and signaling molecules. This interaction determines synaptic strength and plays a role in the modulation of synaptic transmission influencing the communication between neurons.

Pathways

SHANK1 is involved in the glutamatergic synaptic transmission pathway which is important for neural communication and cognitive functions such as learning and memory. Through this pathway SHANK1 interacts with NMDA receptors and mGluR receptors facilitating the propagation of excitatory signals. It also connects with proteins like SAPAP and cortactin which help stabilize the synaptic complex influencing cytoskeletal dynamics and synaptic plasticity.

Associated diseases and disorders

SHANK1 has been implicated in autism spectrum disorders and schizophrenia where disrupted synaptic signaling plays an important role. Mutations in SHANK1 or its regulatory interactions can lead to altered neuronal connectivity and synaptic function which contribute to the pathology of these conditions. The protein's interaction with SHANK3 and other synaptic proteins like neuroligin emphasizes its role in synaptic disorders as these partners also participate in maintaining synaptic structure and function.

Product promise

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4 product images

  • Western blot - Anti-SHANK1 antibody [N22/21] (ab94576), expandable thumbnail

    Western blot - Anti-SHANK1 antibody [N22/21] (ab94576)

    Western blot detection of Shank1 in Rat brain membrane lysates using ab94576 at 1:1000 dilution.

    All lanes: Western blot - Anti-SHANK1 antibody [N22/21] (ab94576)

    Predicted band size: 225 kDa

  • Flow Cytometry - Anti-SHANK1 antibody [N22/21] (ab94576), expandable thumbnail

    Flow Cytometry - Anti-SHANK1 antibody [N22/21] (ab94576)

    Overlay histogram showing SH-SH5Y cells stained with ab94576 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab94576, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in SH-SY5Y cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

  • Immunohistochemistry paraffin embedded sections - Anti-SHANK1 antibody [N22/21] (ab94576), expandable thumbnail

    Immunohistochemistry paraffin embedded sections - Anti-SHANK1 antibody [N22/21] (ab94576)

    paraffin-embedded, bouin's-fixed mouse backskin stained for SHANK1 using ab94576 at 1/100 dilution in immunohistochemical analysis. FITC Goat Anti-Mouse (green) at 1/50 dilution was used as the secondary antibody. Filaggrinlike staining (upper layer aggregations of staining) is observed.

  • Immunocytochemistry/ Immunofluorescence - Anti-SHANK1 antibody [N22/21] (ab94576), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-SHANK1 antibody [N22/21] (ab94576)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed Neuroblastoma cells (SH-SY5Y) labeling SHANK1 with ab94576 at 1/50 dilution. Secondary antibody: AlexaFluor® 488 at 1/1000 dilution. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) SHANK1 Antibody (D) Composite. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) SHANK1 Antibody (D) Composite.

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Product protocols

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