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AB94576

Anti-SHANK1 antibody [N22/21]

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(3 Publications)

Mouse Monoclonal SHANK1 antibody. Suitable for ICC/IF, Flow Cyt, WB, IHC-P and reacts with Human, Rat, Mouse samples. Cited in 3 publications. Immunogen corresponding to Recombinant Protein within Rat Shank1 aa 450-700.

View Alternative Names

SH3 and multiple ankyrin repeat domains protein 1, Shank1, Somatostatin receptor-interacting protein, SSTR-interacting protein, SSTRIP, SHANK1

4 Images
Flow Cytometry - Anti-SHANK1 antibody [N22/21] (AB94576)
  • Flow Cyt

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Flow Cytometry - Anti-SHANK1 antibody [N22/21] (AB94576)

Overlay histogram showing SH-SH5Y cells stained with ab94576 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab94576, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in SH-SY5Y cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Immunocytochemistry/ Immunofluorescence - Anti-SHANK1 antibody [N22/21] (AB94576)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SHANK1 antibody [N22/21] (AB94576)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed Neuroblastoma cells (SH-SY5Y) labeling SHANK1 with ab94576 at 1/50 dilution. Secondary antibody : AlexaFluor® 488 at 1/1000 dilution. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) SHANK1 Antibody (D) Composite. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) SHANK1 Antibody (D) Composite.

Immunohistochemistry paraffin embedded sections - Anti-SHANK1 antibody [N22/21] (AB94576)
  • IHC-P

Supplier Data

Immunohistochemistry paraffin embedded sections - Anti-SHANK1 antibody [N22/21] (AB94576)

paraffin-embedded, bouin's-fixed mouse backskin stained for SHANK1 using ab94576 at 1/100 dilution in immunohistochemical analysis. FITC Goat Anti-Mouse (green) at 1/50 dilution was used as the secondary antibody. Filaggrinlike staining (upper layer aggregations of staining) is observed.

Western blot - Anti-SHANK1 antibody [N22/21] (AB94576)
  • WB

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Western blot - Anti-SHANK1 antibody [N22/21] (AB94576)

Western blot detection of Shank1 in Rat brain membrane lysates using ab94576 at 1 : 1000 dilution.

All lanes:

Western blot - Anti-SHANK1 antibody [N22/21] (ab94576)

Predicted band size: 225 kDa

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Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

N22/21

Isotype

IgG1

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

ICC/IF, IHC-P, Flow Cyt, WB

applications

Immunogen

Recombinant Protein within Rat Shank1 aa 450-700. The exact immunogen used to generate this antibody is proprietary information.

Q9WV48

Specificity

ab94576 does not react with SHANK2 and SHANK3

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry paraffin embedded sections", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "<p></p>", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1 µg for 10^6 Cells", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Rat": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-10 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Product details

The clone number has been updated from S22-21 to N22/21, both clone numbers name the same antibody clone.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Storage buffer
Preservative: 0.09% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SHANK1 also known as SH3 and multiple ankyrin repeat domains protein 1 plays an important role in the structural organization of synapses. It is a scaffold protein found in postsynaptic density where it interacts with other molecules to maintain synaptic architecture. The protein has a molecular mass of approximately 240 kDa. Expression of SHANK1 is most abundant in the brain especially in the hippocampus and cortex where it is essential for establishing and stabilizing synaptic connections during neuronal development.
Biological function summary

SHANK1 participates in the formation of macromolecular complexes that are critical for synaptic signaling and plasticity. It forms complexes with other postsynaptic proteins including PSD-95 and Homer which together coordinate the organization of synaptic receptors and signaling molecules. This interaction determines synaptic strength and plays a role in the modulation of synaptic transmission influencing the communication between neurons.

Pathways

SHANK1 is involved in the glutamatergic synaptic transmission pathway which is important for neural communication and cognitive functions such as learning and memory. Through this pathway SHANK1 interacts with NMDA receptors and mGluR receptors facilitating the propagation of excitatory signals. It also connects with proteins like SAPAP and cortactin which help stabilize the synaptic complex influencing cytoskeletal dynamics and synaptic plasticity.

SHANK1 has been implicated in autism spectrum disorders and schizophrenia where disrupted synaptic signaling plays an important role. Mutations in SHANK1 or its regulatory interactions can lead to altered neuronal connectivity and synaptic function which contribute to the pathology of these conditions. The protein's interaction with SHANK3 and other synaptic proteins like neuroligin emphasizes its role in synaptic disorders as these partners also participate in maintaining synaptic structure and function.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Seems to be an adapter protein in the postsynaptic density (PSD) of excitatory synapses that interconnects receptors of the postsynaptic membrane including NMDA-type and metabotropic glutamate receptors via complexes with GKAP/PSD-95 and Homer, respectively, and the actin-based cytoskeleton. Plays a role in the structural and functional organization of the dendritic spine and synaptic junction.
See full target information SHANK1
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Neurobiology of learning and memory 155:173-179 PubMed30053575

2018

Neocortical SHANK1 regulation of forebrain dependent associative learning.

Applications

Unspecified application

Species

Unspecified reactive species

Sean M Collins,Roberto Galvez

Developmental neurobiology 78:363-373 PubMed29218848

2017

SHANK1 is differentially expressed during development in CA1 hippocampal neurons and astrocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Sean M Collins,Amogh P Belagodu,Samantha L Reed,Roberto Galvez

International journal of immunopathology and pharm 29:217-25 PubMed26957323

2016

Effects of curcumin on synapses in APPswe/PS1dE9 mice.

Applications

Unspecified application

Species

Unspecified reactive species

Yingkun He,Pengwen Wang,Peng Wei,Huili Feng,Ying Ren,Jinduo Yang,Yingxue Rao,Jing Shi,Jinzhou Tian
View all publications
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Product promise

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