Anti-SHANK1 + SHANK2 + SHANK3 antibody [EPR26549-87] (ab317608)

Rabbit Recombinant Monoclonal SHANK2 antibody. Suitable for WB, IHC-Fr, Flow Cyt (Intra), IHC-P and reacts with Recombinant fragment - Human, Human, Mouse, Rat samples.

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Images

Western blot - Anti-SHANK1 + SHANK2 + SHANK3 antibody [EPR26549-87] (AB317608), expandable thumbnail

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	WB	IHC-Fr	Flow Cyt (Intra)	IHC-P	ICC/IF	IP
Human	Tested	Expected	Tested	Not recommended	Not recommended	Not recommended
Mouse	Tested	Tested	Tested	Tested	Not recommended	Not recommended
Rat	Tested	Not recommended	Expected	Tested	Not recommended	Not recommended
Recombinant fragment - Human	Tested	Not recommended	Not recommended	Not recommended	Not recommended	Not recommended

Tested
Tested

Species	Dilution info	Notes
Species Recombinant fragment - Human	Dilution info 1/1000	Notes -
Species Human	Dilution info 1/1000	Notes -
Species Mouse	Dilution info 1/1000	Notes -
Species Rat	Dilution info 1/1000	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/100	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Human	Dilution info Use at an assay dependent concentration.	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Rat, Recombinant fragment - Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/500	Notes -
Species Mouse	Dilution info 1/500	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Rat	Dilution info Use at an assay dependent concentration.	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Recombinant fragment - Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/100	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Rat	Dilution info 1/100	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human, Recombinant fragment - Human	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human, Mouse, Rat, Recombinant fragment - Human	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human, Mouse, Rat, Recombinant fragment - Human	Dilution info -	Notes -

Target data

See full target information SHANK2

Function

Seems to be an adapter protein in the postsynaptic density (PSD) of excitatory synapses that interconnects receptors of the postsynaptic membrane including NMDA-type and metabotropic glutamate receptors, and the actin-based cytoskeleton. May play a role in the structural and functional organization of the dendritic spine and synaptic junction.

Additional Targets

SHANK1, SHANK3

Alternative names

CORTBP1, KIAA1022, PROSAP1, SH3 and multiple ankyrin repeat domains protein 2, Shank2, Cortactin-binding protein 1, Proline-rich synapse-associated protein 1, CortBP1

Recommended products

Rabbit Recombinant Monoclonal SHANK2 antibody. Suitable for WB, IHC-Fr, Flow Cyt (Intra), IHC-P and reacts with Recombinant fragment - Human, Human, Mouse, Rat samples.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EPR26549-87
Purification technique: Affinity purification Protein A
Specificity: Unsuitable for rat IHC-Fr and human IHC-P.
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

SHANK1 SHANK2 and SHANK3 also known as proline-rich synapse-associated protein 1 2 and 3 serve important mechanical roles as scaffolding proteins at the postsynaptic density of excitatory synapses in the brain. SHANK proteins range between approximately 170-220 kDa in mass. They are expressed widely in the brain particularly in regions such as the cerebral cortex and the hippocampus important for higher brain functions. These proteins interact directly with multiple synaptic proteins to maintain synaptic architecture and signal transduction.

Biological function summary

SHANK proteins are integral to the formation and function of synaptic assemblies. They are components of a complex called the postsynaptic density where they link neurotransmitter receptors with cytoskeletal elements. These proteins play an important role in organizing the synaptic machinery necessary for neurotransmission. By anchoring various receptors and proteins at synapses SHANK proteins influence synaptic strength and plasticity essential for learning and memory.

Pathways

SHANK proteins are critical in the N-methyl-D-aspartate receptor (NMDAR) and metabotropic glutamate receptor (mGluR) signaling pathways. SHANK proteins associate with proteins like Homer and GKAP which modulate these pathways. By interacting with these partners SHANK proteins contribute to the regulation of excitatory synaptic transmission and are involved in mechanisms of synaptic plasticity such as long-term potentiation.

Associated diseases and disorders

SHANK proteins are implicated in several neurodevelopmental disorders including autism spectrum disorders (ASD) and schizophrenia. Mutations in SHANK3 in particular have strong associations with ASD. In these disorders altered SHANK function disorganizes synaptic assemblies and signaling leading to neural circuitry dysfunction. In autism dysregulation of SHANK proteins impacts NMDA receptor function which connects also to interaction with proteins like NRXN and GRIN2A contributing to the symptoms associated with these conditions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

15 product images

Western blot - Anti-SHANK1 + SHANK2 + SHANK3 antibody [EPR26549-87] (ab317608)
SHANK1 + SHANK2 + SHANK3 Western blot staining using rabbit Anti-SHANK1 + SHANK2 + SHANK3 antibody
Negative control: skeletal muscle (PMID: 10506216; PMID: 22346768).
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID:10506216). The bands beneath the target band (180-240 kDa) are likely to be degraded target fragments.
In Western blot, Anti-Vinculin antibody [EPR8185] (Anti-Vinculin antibody [EPR8185] - Loading Control ab129002) staining at 1/10000 dilution.
All lanes: Western blot - Anti-SHANK1 + SHANK2 + SHANK3 antibody [EPR26549-87] (ab317608) at 1/1000 dilution
Lane 1: Human hippocampus tissue lysate at 20 µg with NFDM/TBST
Lane 2: Human skeletal muscle tissue lysate at 20 µg with NFDM/TBST
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/200000 dilution
Observed band size: 180-240 kDa, 124 kDa
Exposure time: 180s
Western blot - Anti-SHANK1 + SHANK2 + SHANK3 antibody [EPR26549-87] (ab317608)
SHANK1 + SHANK2 + SHANK3 Western blot staining using rabbit Anti-SHANK1 + SHANK2 + SHANK3 antibody
Low expression: 293T (PMID:32661924).
The molecular weight observed is consistent with what has been described in the literature (PMID:10506216). The bands beneath the target band (180-270 kDa) are likely to be degraded target fragments.
In Western blot, Anti-Vinculin antibody [EPR8185] (Anti-Vinculin antibody [EPR8185] - Loading Control ab129002) staining at 1/10000 dilution.
All lanes: Western blot - Anti-SHANK1 + SHANK2 + SHANK3 antibody [EPR26549-87] (ab317608) at 1/1000 dilution
Lane 1: T-47D (human ductal breast epithelial tumor epithelial cell) whole cell lysate at 20 µg with NFDM/TBST
Lane 2: 293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg with NFDM/TBST
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 180-270 kDa, 124 kDa
Exposure time: 15s
Western blot - Anti-SHANK1 + SHANK2 + SHANK3 antibody [EPR26549-87] (ab317608)
SHANK1 + SHANK2 + SHANK3 Western blot staining using rabbit Anti-SHANK1 + SHANK2 + SHANK3 antibody
Negative control: skeletal muscle (PMID: 10506216; PMID: 22346768).
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID:10506216). The bands beneath the target band (180-270 kDa) are likely to be degraded target fragments.
In Western blot, Anti-Vinculin antibody [EPR8185] (Anti-Vinculin antibody [EPR8185] - Loading Control ab129002) staining at 1/10000 dilution.
All lanes: Western blot - Anti-SHANK1 + SHANK2 + SHANK3 antibody [EPR26549-87] (ab317608) at 1/1000 dilution
Lane 1: Mouse cerebellum tissue lysate at 20 µg with NFDM/TBST
Lane 2: Mouse hippocampus tissue lysate at 20 µg with NFDM/TBST
Lane 3: Mouse skeletal muscle tissue lysate at 20 µg with NFDM/TBST
Lane 4: Rat cerebellum tissue lysate at 20 µg with NFDM/TBST
Lane 5: Rat hippocampus tissue lysate at 20 µg with NFDM/TBST
Lane 6: Rat skeletal muscle tissue lysate at 20 µg with NFDM/TBST
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 180-270 kDa, 124 kDa
Exposure time: 59s
Western blot - Anti-SHANK1 + SHANK2 + SHANK3 antibody [EPR26549-87] (ab317608)
This antibody can recognize human SHANK1, SHANK2 and SHANK3. .
In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution.
All lanes: Western blot - Anti-SHANK1 + SHANK2 + SHANK3 antibody [EPR26549-87] (ab317608) at 1/1000 dilution
Lane 1: His-tagged human SHANK2 recombinant fragment at 10 ng with NFDM/TBST
Lane 2: His-tagged human SHANK1 recombinant fragment at 10 ng with NFDM/TBST
Lane 3: His-tagged human SHANK3 recombinant fragment at 10 ng with NFDM/TBST
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 26 kDa
Exposure time: 125s
Flow Cytometry (Intracellular) - Anti-SHANK1 + SHANK2 + SHANK3 antibody [EPR26549-87] (ab317608)
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Mouse spleen cell cells labelling SHANK1 + SHANK2 + SHANK3 with ab317608 at 1/500 dilution (0.1ug)/right (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.
Negative control: mouse spleen cell.
Flow Cytometry (Intracellular) - Anti-SHANK1 + SHANK2 + SHANK3 antibody [EPR26549-87] (ab317608)
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Mouse primary neuron cells labelling SHANK1 + SHANK2 + SHANK3 with ab317608 at 1/500 dilution (0.1ug)/right (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.
Flow Cytometry (Intracellular) - Anti-SHANK1 + SHANK2 + SHANK3 antibody [EPR26549-87] (ab317608)
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized T-47D (human ductal breast epithelial tumor epithelial cell) (Right) / 293T (human embryonic kidney epithelial cell) (Left) cells labelling SHANK1 + SHANK2 + SHANK3 with ab317608 at 1/500 dilution (0.1ug)/Red (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.
Low expression: 293T (PMID: 32661924)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHANK1 + SHANK2 + SHANK3 antibody [EPR26549-87] (ab317608)
Immunohistochemical analysis of paraffin-embedded Rat skeletal muscle tissue labeling SHANK1 + SHANK2 + SHANK3 with ab317608 at 1/100 (4.75 ug/ml) dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Negative control: No staining on rat skeletal muscle (PMID: 10506216). The section was incubated with ab317608 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHANK1 + SHANK2 + SHANK3 antibody [EPR26549-87] (ab317608)
Immunohistochemical analysis of paraffin-embedded Mouse skeletal muscle tissue labeling SHANK1 + SHANK2 + SHANK3 with ab317608 at 1/100 (4.75 ug/ml) dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Negative control: No staining on mouse skeletal muscle (PMID: 10506216). The section was incubated with ab317608 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHANK1 + SHANK2 + SHANK3 antibody [EPR26549-87] (ab317608)
Immunohistochemical analysis of paraffin-embedded Rat cerebellum tissue labeling SHANK1 + SHANK2 + SHANK3 with ab317608 at 1/100 (4.75 ug/ml) dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Positive staining in rat cerebellum (PMID: 27581745). The section was incubated with ab317608 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHANK1 + SHANK2 + SHANK3 antibody [EPR26549-87] (ab317608)
Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling SHANK1 + SHANK2 + SHANK3 with ab317608 at 1/100 (4.75 ug/ml) dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Positive staining on cholangiocytes in rat liver (PMID: 14977424). The section was incubated with ab317608 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHANK1 + SHANK2 + SHANK3 antibody [EPR26549-87] (ab317608)
Immunohistochemical analysis of paraffin-embedded Mouse hippocampus tissue labeling SHANK1 + SHANK2 + SHANK3 with ab317608 at 1/100 (4.75 ug/ml) dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Positive staining in mouse hippocampus (PMID: 27581745). The section was incubated with ab317608 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Immunohistochemistry (Frozen sections) - Anti-SHANK1 + SHANK2 + SHANK3 antibody [EPR26549-87] (ab317608)
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse skeletal muscle (fresh frozen) tissue labeling SHANK1 + SHANK2 + SHANK3 with ab317608 at 1/100 (4.75 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green).
Negative control: confocal image showing no staining on mouse skeletal muscle. The nuclear counterstain was DAPI (Blue). The section was incubated with ab317608 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 2 ug/mL dilution.
Immunohistochemistry (Frozen sections) - Anti-SHANK1 + SHANK2 + SHANK3 antibody [EPR26549-87] (ab317608)
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse hippocampus (fresh frozen) tissue labeling SHANK1 + SHANK2 + SHANK3 with ab317608 at 1/100 (4.75 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green).
Confocal image showing positive staining on mouse hippocampus. The nuclear counterstain was DAPI (Blue). The section was incubated with ab317608 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 2 ug/mL dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHANK1 + SHANK2 + SHANK3 antibody [EPR26549-87] (ab317608)
Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling SHANK1 + SHANK2 + SHANK3 with ab317608 at 1/100 (4.75 ug/ml) dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Positive staining on cholangiocytes in mouse liver (PMID: 14977424). The section was incubated with ab317608 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins