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Rabbit Recombinant Monoclonal SHANK2 antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Mouse, Rat samples.

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Images

Western blot - Anti-SHANK2 antibody [EPR24142-35] (AB259966), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-SHANK2 antibody [EPR24142-35] (AB259966), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-SHANK2 antibody [EPR24142-35] (AB259966), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHANK2 antibody [EPR24142-35] (AB259966), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHANK2 antibody [EPR24142-35] (AB259966), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPFlow CytWBICC/IFIHC-FrIHC-P
Human
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Mouse
Not recommended
Not recommended
Tested
Not recommended
Not recommended
Tested
Rat
Not recommended
Not recommended
Tested
Tested
Not recommended
Tested

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/1000
Notes

-

Species
Rat
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Rat
Dilution info
1 µg/mL
Notes

-

Not recommended
Not recommended

Species
Mouse, Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/5000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
1/5000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target data

Function

Seems to be an adapter protein in the postsynaptic density (PSD) of excitatory synapses that interconnects receptors of the postsynaptic membrane including NMDA-type and metabotropic glutamate receptors, and the actin-based cytoskeleton. May play a role in the structural and functional organization of the dendritic spine and synaptic junction (By similarity).

Alternative names

Cortbp1, Kiaa1022, Shank2, SH3 and multiple ankyrin repeat domains protein 2, Cortactin-binding protein 1, CortBP1

Recommended products

Rabbit Recombinant Monoclonal SHANK2 antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Mouse, Rat samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR24142-35
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

SHANK2 also known as SH3 and multiple ankyrin repeat domains protein 2 is a scaffold protein of around 189 kDa. It is mainly expressed in the central nervous system especially in postsynaptic densities of dendritic spines in neurons. SHANK2 interacts with several synaptic proteins to organize the synaptic complexes that are important for neurotransmission. It helps in maintaining the structural integrity and signaling functions of synapses.

Biological function summary

SHANK2 provides key links in postsynaptic density structures. It interacts with other proteins like neuroligins and NMDA receptors to form a complex signaling network. This complex is involved in synaptic signal transduction which is critical for neural plasticity and communication between neurons. Through its role in synapses SHANK2 influences the strength and modulation of synaptic transmission.

Pathways

SHANK2 plays a significant role in neurotransmitter signaling pathways such as glutamatergic signaling. It directly interacts with proteins like Homer and mGluR that are critical to these pathways. These interactions influence synaptic growth and plasticity impacting cognitive processes like learning and memory. The proper functioning of these pathways depends on SHANK2's stability and interactions with other postsynaptic proteins.

Associated diseases and disorders

Alterations in SHANK2 are linked to conditions like autism spectrum disorders and schizophrenia. Mutations in SHANK2 affect synaptic functions which are often seen in these neurological conditions. Additionally SHANK2 dysfunction relates to neuroligins and other postsynaptic proteins highlighting its involvement in the pathophysiology of these disorders. Understanding SHANK2's role can help in developing targeted therapies for such conditions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Western blot - Anti-SHANK2 antibody [EPR24142-35] (ab259966), expandable thumbnail

    Western blot - Anti-SHANK2 antibody [EPR24142-35] (ab259966)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Shank2 is expressed predominately in brain tissue what has described in the literature. (PMID: 9742101).

    Exposure time: 3 minutes.

    All lanes: Western blot - Anti-SHANK2 antibody [EPR24142-35] (ab259966) at 1/1000 dilution

    Lane 1: Mouse hippocampus tissue lysate at 20 µg

    Lane 2: Rat brain tissue lysate at 20 µg

    Lane 3: Rat hippocampus tissue lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 165 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-SHANK2 antibody [EPR24142-35] (ab259966), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-SHANK2 antibody [EPR24142-35] (ab259966)

    Immunofluorescence staining of SHANK2 using ab259966 in primary hippocampal rat neurons/glia, (obtained from Neuromics, cat. no. PC35101), DIV14. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% PBS-Tween for 5 mins and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab259966 at 1 μg/ml and Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291, Mouse monoclonal [DM1A] to alpha Tubulin, at 1/1000 dilution. Cells were then incubated with Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (shown in green) and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120, Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed at 1/1000 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

    Images were acquired with the Perkin Elmer Operetta HCA and a maximum intensity projection of confocal sections is shown.

  • Immunocytochemistry/ Immunofluorescence - Anti-SHANK2 antibody [EPR24142-35] (ab259966), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-SHANK2 antibody [EPR24142-35] (ab259966)

    Immunofluorescence staining of SHANK2 using ab259966 in primary hippocampal rat neurons/glia, (obtained from Neuromics, cat. no. PC35101), DIV14. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Tween for 5 mins and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab259966 at 0.1 μg/ml and Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291, Mouse monoclonal [DM1A] to alpha Tubulin, at 1/1000 dilution. Cells were then incubated with Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (shown in green) and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120, Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed at 1/1000 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

    Images were acquired with the Perkin Elmer Operetta HCA and a maximum intensity projection of confocal sections is shown.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHANK2 antibody [EPR24142-35] (ab259966), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHANK2 antibody [EPR24142-35] (ab259966)

    Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling SHANK2 with ab259966 at 1/5000 (0.0924 μg/ml) followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Cytoplasmic stainig on mouse cerebrum (PMID:29572432, 28179641). The section was incubated with ab259966 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHANK2 antibody [EPR24142-35] (ab259966), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHANK2 antibody [EPR24142-35] (ab259966)

    Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue labeling SHANK2 with ab259966 at 1/5000 (0.0924 μg/ml) followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Cytoplasmic stainig on mouse hippocampus (PMID: 27581745, 29572432, 28179641). The section was incubated with ab259966 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHANK2 antibody [EPR24142-35] (ab259966), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHANK2 antibody [EPR24142-35] (ab259966)

    Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling SHANK2 with ab259966 at 1/5000 (0.0924 μg/ml) followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Positive staining on bile duct in rat liver (PMID: 14977424). The section was incubated with ab259966 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHANK2 antibody [EPR24142-35] (ab259966), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHANK2 antibody [EPR24142-35] (ab259966)

    Immunohistochemical analysis of paraffin-embedded rat hippocampus tissue labeling SHANK2 with ab259966 at 1/5000 (0.0924 μg/ml) followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Cytoplasmic stainig on rat hippocampus. The section was incubated with ab259966 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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