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Rabbit Recombinant Monoclonal SHANK2 antibody. Suitable for WB, IHC-Fr, Flow Cyt (Intra), IHC-P and reacts with Recombinant fragment - Human, Human, Mouse, Rat samples.


Images

Western blot - Anti-SHANK2 antibody [EPR26549-331] (AB317606), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-SHANK2 antibody [EPR26549-331] (AB317606), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-SHANK2 antibody [EPR26549-331] (AB317606), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-SHANK2 antibody [EPR26549-331] (AB317606), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-SHANK2 antibody [EPR26549-331] (AB317606), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-FrFlow Cyt (Intra)IHC-PICC/IFIP
Human
Tested
Expected
Tested
Not recommended
Not recommended
Not recommended
Mouse
Tested
Tested
Tested
Tested
Not recommended
Not recommended
Rat
Tested
Tested
Tested
Tested
Not recommended
Not recommended
Recombinant fragment - Human
Tested
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species

Recombinant fragment - Human

Dilution info

1/1000

Notes

-

Species

Human

Dilution info

1/1000

Notes

-

Species

Mouse

Dilution info

1/1000

Notes

-

Species

Rat

Dilution info

1/1000

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/100

Notes

-

Species

Rat

Dilution info

1/100

Notes

-

Expected
Expected

Species

Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Recombinant fragment - Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/500

Notes

-

Species

Mouse

Dilution info

1/500

Notes

-

Species

Rat

Dilution info

1/500

Notes

-

Not recommended
Not recommended

Species

Recombinant fragment - Human

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/100

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Rat

Dilution info

1/100

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species

Human, Recombinant fragment - Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Mouse, Rat, Recombinant fragment - Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Mouse, Rat, Recombinant fragment - Human

Dilution info

-

Notes

-

Target data

Function

Seems to be an adapter protein in the postsynaptic density (PSD) of excitatory synapses that interconnects receptors of the postsynaptic membrane including NMDA-type and metabotropic glutamate receptors, and the actin-based cytoskeleton. May play a role in the structural and functional organization of the dendritic spine and synaptic junction.

Alternative names

Recommended products

Rabbit Recombinant Monoclonal SHANK2 antibody. Suitable for WB, IHC-Fr, Flow Cyt (Intra), IHC-P and reacts with Recombinant fragment - Human, Human, Mouse, Rat samples.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR26549-331

Purification technique

Affinity purification Protein A

Specificity

Unsuitable for human IHC-P.

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

SHANK2 also known as SH3 and multiple ankyrin repeat domains protein 2 is a scaffold protein of around 189 kDa. It is mainly expressed in the central nervous system especially in postsynaptic densities of dendritic spines in neurons. SHANK2 interacts with several synaptic proteins to organize the synaptic complexes that are important for neurotransmission. It helps in maintaining the structural integrity and signaling functions of synapses.

Biological function summary

SHANK2 provides key links in postsynaptic density structures. It interacts with other proteins like neuroligins and NMDA receptors to form a complex signaling network. This complex is involved in synaptic signal transduction which is critical for neural plasticity and communication between neurons. Through its role in synapses SHANK2 influences the strength and modulation of synaptic transmission.

Pathways

SHANK2 plays a significant role in neurotransmitter signaling pathways such as glutamatergic signaling. It directly interacts with proteins like Homer and mGluR that are critical to these pathways. These interactions influence synaptic growth and plasticity impacting cognitive processes like learning and memory. The proper functioning of these pathways depends on SHANK2's stability and interactions with other postsynaptic proteins.

Associated diseases and disorders

Alterations in SHANK2 are linked to conditions like autism spectrum disorders and schizophrenia. Mutations in SHANK2 affect synaptic functions which are often seen in these neurological conditions. Additionally SHANK2 dysfunction relates to neuroligins and other postsynaptic proteins highlighting its involvement in the pathophysiology of these disorders. Understanding SHANK2's role can help in developing targeted therapies for such conditions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

19 product images

  • Western blot - Anti-SHANK2 antibody [EPR26549-331] (ab317606), expandable thumbnail

    Western blot - Anti-SHANK2 antibody [EPR26549-331] (ab317606)

    This antibody does not cross-react with human SHANK1 and SHANK3.

    In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution.

    All lanes: Western blot - Anti-SHANK2 antibody [EPR26549-331] (ab317606) at 1/1000 dilution

    Lane 1: His-tagged human SHANK2 recombinant fragment at 10 ng

    Lane 2: His-tagged human SHANK1 recombinant fragment at 10 ng

    Lane 3: His-tagged human SHANK3 recombinant fragment at 10 ng

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 26 kDa

    Exposure time: 70s

  • Flow Cytometry (Intracellular) - Anti-SHANK2 antibody [EPR26549-331] (ab317606), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-SHANK2 antibody [EPR26549-331] (ab317606)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Rat spleen cell cells labelling SHANK2 with ab317606 at 1/500 dilution (0.1ug)/right (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

    Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.

    Negative control: rat spleen cell.

  • Flow Cytometry (Intracellular) - Anti-SHANK2 antibody [EPR26549-331] (ab317606), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-SHANK2 antibody [EPR26549-331] (ab317606)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Rat primary neuron cells labelling SHANK2 with ab317606 at 1/500 dilution (0.1ug)/right (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

    Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.

  • Flow Cytometry (Intracellular) - Anti-SHANK2 antibody [EPR26549-331] (ab317606), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-SHANK2 antibody [EPR26549-331] (ab317606)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Mouse spleen cell cells labelling SHANK2 with ab317606 at 1/500 dilution (0.1ug)/right (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

    Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.

    Negative control: mouse spleen cell.

  • Flow Cytometry (Intracellular) - Anti-SHANK2 antibody [EPR26549-331] (ab317606), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-SHANK2 antibody [EPR26549-331] (ab317606)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Mouse primary neuron cells labelling SHANK2 with ab317606 at 1/500 dilution (0.1ug)/right (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

    Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.

  • Flow Cytometry (Intracellular) - Anti-SHANK2 antibody [EPR26549-331] (ab317606), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-SHANK2 antibody [EPR26549-331] (ab317606)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized T-47D (human ductal breast epithelial tumor epithelial cell) (Right) / 293T (human embryonic kidney epithelial cell) (Left) cells labelling SHANK2 with ab317606 at 1/500 dilution (0.1ug)/Red (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

    Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.

    Low expression: 293T(PMID: 32661924)

  • Immunohistochemistry (Frozen sections) - Anti-SHANK2 antibody [EPR26549-331] (ab317606), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-SHANK2 antibody [EPR26549-331] (ab317606)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat skeletal muscle (fresh frozen) tissue labeling SHANK2 with ab317606 at 1/100 (5.21 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green).

    Negative control: confocal image showing no staining on rat skeletal muscle. The nuclear counterstain was DAPI (Blue). The section was incubated with ab317606 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 2 ug/mL dilution.

  • Immunohistochemistry (Frozen sections) - Anti-SHANK2 antibody [EPR26549-331] (ab317606), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-SHANK2 antibody [EPR26549-331] (ab317606)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat hippocampus (fresh frozen) tissue labeling SHANK2 with ab317606 at 1/100 (5.21 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green).

    Confocal image showing positive staining on rat hippocampus. The nuclear counterstain was DAPI (Blue). The section was incubated with ab317606 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 2 ug/mL dilution.

  • Immunohistochemistry (Frozen sections) - Anti-SHANK2 antibody [EPR26549-331] (ab317606), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-SHANK2 antibody [EPR26549-331] (ab317606)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse skeletal muscle (fresh frozen) tissue labeling SHANK2 with ab317606 at 1/100 (5.21 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green).

    Negative control: confocal image showing no staining on mouse skeletal muscle. The nuclear counterstain was DAPI (Blue). The section was incubated with ab317606 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 2 ug/mL dilution.

  • Immunohistochemistry (Frozen sections) - Anti-SHANK2 antibody [EPR26549-331] (ab317606), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-SHANK2 antibody [EPR26549-331] (ab317606)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse hippocampus (fresh frozen) tissue labeling SHANK2 with ab317606 at 1/100 (5.21 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green).

    Confocal image showing positive staining on mouse hippocampus. The nuclear counterstain was DAPI (Blue). The section was incubated with ab317606 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 2 ug/mL dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHANK2 antibody [EPR26549-331] (ab317606), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHANK2 antibody [EPR26549-331] (ab317606)

    Immunohistochemical analysis of paraffin-embedded Rat skeletal muscle tissue labeling SHANK2 with ab317606 at 1/100 (5.21 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Negative control: no staining on rat skeletal muscle. The section was incubated with ab317606 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHANK2 antibody [EPR26549-331] (ab317606), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHANK2 antibody [EPR26549-331] (ab317606)

    Immunohistochemical analysis of paraffin-embedded Mouse skeletal muscle tissue labeling SHANK2 with ab317606 at 1/100 (5.21 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Negative control: no staining on mouse skeletal muscle. The section was incubated with ab317606 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHANK2 antibody [EPR26549-331] (ab317606), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHANK2 antibody [EPR26549-331] (ab317606)

    Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling SHANK2 with ab317606 at 1/100 (5.21 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on cholangiocytes of rat liver. The section was incubated with ab317606 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHANK2 antibody [EPR26549-331] (ab317606), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHANK2 antibody [EPR26549-331] (ab317606)

    Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling SHANK2 with ab317606 at 1/100 (5.21 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on rat cerebrum. The section was incubated with ab317606 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHANK2 antibody [EPR26549-331] (ab317606), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHANK2 antibody [EPR26549-331] (ab317606)

    Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling SHANK2 with ab317606 at 1/100 (5.21 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on cholangiocytes of mouse liver. The section was incubated with ab317606 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHANK2 antibody [EPR26549-331] (ab317606), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHANK2 antibody [EPR26549-331] (ab317606)

    Immunohistochemical analysis of paraffin-embedded Mouse hippocampus tissue labeling SHANK2 with ab317606 at 1/100 (5.21 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on mouse hippocampus. The section was incubated with ab317606 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Western blot - Anti-SHANK2 antibody [EPR26549-331] (ab317606), expandable thumbnail

    Western blot - Anti-SHANK2 antibody [EPR26549-331] (ab317606)

    Negative control: skeletal muscle (PMID: 10506216; PMID: 22346768).

    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID:29572432). The bands beneath the target band (180-270 kDa) are likely to be degraded target fragments.

    In Western blot, Anti-Vinculin antibody [EPR8185] (Anti-Vinculin antibody [EPR8185] ab129002) staining at 1/10000 dilution.

    All lanes: Western blot - Anti-SHANK2 antibody [EPR26549-331] (ab317606) at 1/1000 dilution

    Lane 1: Mouse hippocampus tissue lysate at 20 µg

    Lane 2: Mouse cerebellum tissue lysate at 20 µg

    Lane 3: Mouse skeletal muscle tissue lysate at 20 µg

    Lane 4: Rat hippocampus tissue lysate at 20 µg

    Lane 5: Rat cerebellum tissue lysate at 20 µg

    Lane 6: Rat skeletal muscle tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 180-270 kDa, 180 kDa, 124 kDa

    Exposure time: 180s

  • Western blot - Anti-SHANK2 antibody [EPR26549-331] (ab317606), expandable thumbnail

    Western blot - Anti-SHANK2 antibody [EPR26549-331] (ab317606)

    Low expression: 293T (PMID:32661924).

    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID:29572432). The bands beneath the target band (180-270 kDa) are likely to be degraded target fragments.

    In Western blot, Anti-Vinculin antibody [EPR8185] (Anti-Vinculin antibody [EPR8185] ab129002) staining at 1/10000 dilution.

    All lanes: Western blot - Anti-SHANK2 antibody [EPR26549-331] (ab317606) at 1/1000 dilution

    Lane 1: T-47D (human ductal breast epithelial tumor epithelial cell) whole cell lysate at 20 µg

    Lane 2: 293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 180-270 kDa, 124 kDa

    Exposure time: 26s

  • Western blot - Anti-SHANK2 antibody [EPR26549-331] (ab317606), expandable thumbnail

    Western blot - Anti-SHANK2 antibody [EPR26549-331] (ab317606)

    Negative control: skeletal muscle (PMID: 10506216; PMID: 22346768).

    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID:29572432). The bands beneath the target band (180-200 kDa) are likely to be degraded target fragments.

    In Western blot, Anti-Vinculin antibody [EPR8185] (Anti-Vinculin antibody [EPR8185] ab129002) staining at 1/10000 dilution.

    All lanes: Western blot - Anti-SHANK2 antibody [EPR26549-331] (ab317606) at 1/1000 dilution

    Lane 1: Human hippocampus tissue lysate at 20 µg

    Lane 2: Human skeletal muscle tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/200000 dilution

    Observed band size: 200 kDa, 180 kDa, 124 kDa

    Exposure time: 180s

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Product protocols

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