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Knockout Tested Mouse Monoclonal SHANK2 antibody. Suitable for WB, ICC/IF and reacts with Human, Rat samples. Cited in 3 publications. Immunogen corresponding to Recombinant Protein within Rat Shank2 aa 50-350.

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Images

Immunocytochemistry/ Immunofluorescence - Anti-SHANK2 antibody [N23B/49] (AB94575), expandable thumbnail
  • Western blot - Anti-SHANK2 antibody [N23B/49] (AB94575), expandable thumbnail
  • Western blot - Anti-SHANK2 antibody [N23B/49] (AB94575), expandable thumbnail

Publications

Key facts

Isotype
IgG1
Host species
Mouse
Storage buffer

Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Form
Liquid
Clonality
Monoclonal

Immunogen

  • Recombinant Protein within Rat Shank2 aa 50-350. The exact immunogen used to generate this antibody is proprietary information. Database link Q9QX74

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IF
Human
Tested
Tested
Rat
Tested
Expected

Tested
Tested

Species
Human
Dilution info
1-10 µg/mL
Notes

-

Species
Rat
Dilution info
1.00000-10.00000 µg/mL
Notes

-

Tested
Tested

Species
Human
Dilution info
1.00000-10.00000 µg/mL
Notes

-

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Target data

Function

Seems to be an adapter protein in the postsynaptic density (PSD) of excitatory synapses that interconnects receptors of the postsynaptic membrane including NMDA-type and metabotropic glutamate receptors, and the actin-based cytoskeleton. May play a role in the structural and functional organization of the dendritic spine and synaptic junction.

Alternative names

Cortbp1, Shank2, SH3 and multiple ankyrin repeat domains protein 2, Cortactin-binding protein 1, GKAP/SAPAP-interacting protein, Proline-rich synapse-associated protein 1, SPANK-3, CortBP1, ProSAP1

Recommended products

Knockout Tested Mouse Monoclonal SHANK2 antibody. Suitable for WB, ICC/IF and reacts with Human, Rat samples. Cited in 3 publications. Immunogen corresponding to Recombinant Protein within Rat Shank2 aa 50-350.

Key facts

Isotype
IgG1
Form
Liquid
Clonality
Monoclonal
Immunogen
  • Recombinant Protein within Rat Shank2 aa 50-350. The exact immunogen used to generate this antibody is proprietary information. Database link Q9QX74
Clone number
N23B/49
Purification technique
Affinity purification Protein G
Specificity

Recognizes SHANK1, SHANK2, SHANK3.

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

The clone number has been updated from S23B-49 to N23B/49, both clone numbers name the same antibody clone.

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Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

SHANK2 also known as SH3 and multiple ankyrin repeat domains protein 2 is a scaffold protein of around 189 kDa. It is mainly expressed in the central nervous system especially in postsynaptic densities of dendritic spines in neurons. SHANK2 interacts with several synaptic proteins to organize the synaptic complexes that are important for neurotransmission. It helps in maintaining the structural integrity and signaling functions of synapses.

Biological function summary

SHANK2 provides key links in postsynaptic density structures. It interacts with other proteins like neuroligins and NMDA receptors to form a complex signaling network. This complex is involved in synaptic signal transduction which is critical for neural plasticity and communication between neurons. Through its role in synapses SHANK2 influences the strength and modulation of synaptic transmission.

Pathways

SHANK2 plays a significant role in neurotransmitter signaling pathways such as glutamatergic signaling. It directly interacts with proteins like Homer and mGluR that are critical to these pathways. These interactions influence synaptic growth and plasticity impacting cognitive processes like learning and memory. The proper functioning of these pathways depends on SHANK2's stability and interactions with other postsynaptic proteins.

Associated diseases and disorders

Alterations in SHANK2 are linked to conditions like autism spectrum disorders and schizophrenia. Mutations in SHANK2 affect synaptic functions which are often seen in these neurological conditions. Additionally SHANK2 dysfunction relates to neuroligins and other postsynaptic proteins highlighting its involvement in the pathophysiology of these disorders. Understanding SHANK2's role can help in developing targeted therapies for such conditions.

Product promise

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3 product images

  • Immunocytochemistry/ Immunofluorescence - Anti-SHANK2 antibody [N23B/49] (ab94575), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-SHANK2 antibody [N23B/49] (ab94575)

    4% formaldehyde-fixed (for 15 minutes at room temperature) SK-N-BE (Human neuroblastoma cells) labeling SHANK2 using ab94575 at 1/100 dilution (for 60 minutes at room temperature). Goat Anti-Mouse ATTO 488 was used as the secondary at 1/200 dilution for 60 minutes at room temperature. Localization in the sytplasm is observed.

    Counterstains: Phalloidin Texas Red F-Actin stain (Red). DAPI nuclear stain (Blue).

  • Western blot - Anti-SHANK2 antibody [N23B/49] (ab94575), expandable thumbnail

    Western blot - Anti-SHANK2 antibody [N23B/49] (ab94575)

    Western blot detection of SHANK2 in Rat braim membrane lysates using ab94575 at 1:1000 dilution.

    All lanes: Western blot - Anti-SHANK2 antibody [N23B/49] (ab94575)

  • Western blot - Anti-SHANK2 antibody [N23B/49] (ab94575), expandable thumbnail

    Western blot - Anti-SHANK2 antibody [N23B/49] (ab94575)

    Western blot: Anti-SHANK2 antibody [N23B/49] (ab94575) staining at 1 ug/ml, shown in green; Rabbit anti-ACTN4 [EPR2533(2)] (Anti-alpha Actinin 4 antibody [EPR2533(2)] ab108198) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, ab94575 was shown to bind specifically to SHANK2. A band was observed at 201 kDa in wild-type A549 cell lysates with no signal observed at this size in SHANK2 knockout cell line. To generate this image, wild-type and SHANK2 knockout A549 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Mouse IgG H&L 800CW and Goat anti-Rabbit IgG H&L 680RD at 1/20000 dilution.

    All lanes: Western blot - Anti-SHANK2 antibody [N23B/49] (ab94575) at 1 µg/mL

    Lane 1: Wild-type A549 cell lysate at 20 µg

    Lane 2: SHANK2 knockout A549 cell lysate at 20 µg

    Secondary

    All lanes: Goat anti-Mouse IgG H&L 800CW and Goat anti-Rabbit IgG H&L 680RD at 1/20000 dilution

    Performed under reducing conditions.

    Predicted band size: 158 kDa

    Observed band size: 201 kDa

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Product protocols

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