AB264347

Anti-SHANK3 antibody

Name: Anti-SHANK3 antibody (ab264347) | Abcam
Brand: Abcam Limited
SKU: AB264347

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(2 Publications)

Rabbit Polyclonal SHANK3 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human SHANK3 aa 1450-1550.

View Alternative Names

KIAA1650, PROSAP2, PSAP2, SHANK3, SH3 and multiple ankyrin repeat domains protein 3, Shank3, Proline-rich synapse-associated protein 2, ProSAP2

2 Images

Supplier Data

Immunoprecipitation - Anti-SHANK3 antibody (AB264347)

SHANK3 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) using ab264347 at 6 μg/mg lysate. Western blot was performed on the immunoprecipitates using ab264347 at 1 μg/ml.

Lane 1 : ab264347 IP in HeLa whole cell lysate.

Lane 2 : Control IgG IP in Hela whole cell lysate.

Detection : Chemiluminescence with an exposure time of 10 seconds.

All lanes:

Immunoprecipitation - Anti-SHANK3 antibody (ab264347)

Predicted band size: 186 kDa

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Supplier Data

Western blot - Anti-SHANK3 antibody (AB264347)

All lanes:

Western blot - Anti-SHANK3 antibody (ab264347) at 0.1 µg/mL

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Lane 3:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg

Lane 4:

TCMK-1 (mouse kidney epithelial cell line) whole cell lysate at 50 µg

Lane 5:

NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 50 µg

Predicted band size: 186 kDa

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Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human SHANK3 aa 1450-1550. The exact immunogen used to generate this antibody is proprietary information.

Q9BYB0

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The SHANK3 protein also known as ProSAP2 is a scaffold protein that plays an important role at the postsynaptic density of excitatory synapses. It has a mass of around 240 kDa. SHANK3 is expressed mainly in the brain but also present in heart spleen and kidneys. It interacts with various proteins through its multiple domains contributing to the structural integrity and signaling functions of synapses.

Biological function summary

SHANK3 connects the cytoskeleton to the membrane proteins maintaining synaptic structure and function. As a part of the postsynaptic density complex SHANK3 interacts with proteins like Homer and NMDA receptors to organize the signaling platforms. Its role in synaptic structure regulation influences neuronal connectivity which impacts brain function and development.

Pathways

SHANK3 interacts with the signaling cascade involved in synaptic plasticity and communication. Specifically it is important in the mTOR pathway which influences cell growth and synaptic protein synthesis and the glutamatergic synapse pathway essential for synaptic transmission. Within these pathways SHANK3 closely associates with proteins such as PSD-95 and SAPAP facilitating synaptic signal transduction.

SHANK3 mutations link to neurodevelopmental disorders including autism spectrum disorder (ASD) and Phelan-McDermid syndrome. These mutations can disrupt its interaction with proteins like PSD-95 affecting synaptic stability and leading to cognitive and social impairments. SHANK3's role in these conditions highlights the importance of proper synaptic signaling and structure for normal neurological function.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Major scaffold postsynaptic density protein which interacts with multiple proteins and complexes to orchestrate the dendritic spine and synapse formation, maturation and maintenance. Interconnects receptors of the postsynaptic membrane including NMDA-type and metabotropic glutamate receptors via complexes with GKAP/PSD-95 and HOMER, respectively, and the actin-based cytoskeleton. Plays a role in the structural and functional organization of the dendritic spine and synaptic junction through the interaction with Arp2/3 and WAVE1 complex as well as the promotion of the F-actin clusters. By way of this control of actin dynamics, participates in the regulation of developing neurons growth cone motility and the NMDA receptor-signaling. Also modulates GRIA1 exocytosis and GRM5/MGLUR5 expression and signaling to control the AMPA and metabotropic glutamate receptor-mediated synaptic transmission and plasticity. May be required at an early stage of synapse formation and be inhibited by IGF1 to promote synapse maturation.

See full target information SHANK3

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Neuroscience bulletin 41:583-599 PubMed39693031

2024

Deciphering the Role of Shank3 in Dendritic Morphology and Synaptic Function Across Postnatal Developmental Stages in the Shank3B KO Mouse.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Yang,Guaiguai Ma,Xiaohui Du,Jinyi Xie,Mengmeng Wang,Wenting Wang,Baolin Guo,Shengxi Wu

Redox biology 58:102537 PubMed36436456

2022

Ablation of Shank3 alleviates cardiac dysfunction in aging mice by promoting CaMKII activation and Parkin-mediated mitophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Wang,Yuerong Xu,Wangang Guo,Yexian Fang,Lang Hu,Runze Wang,Ran Zhao,Dong Guo,Bingchao Qi,Gaotong Ren,Jun Ren,Yan Li,Mingming Zhang

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-SHANK3 antibody

Immunoprecipitation - Anti-SHANK3 antibody (AB264347)

Western blot - Anti-SHANK3 antibody (AB264347)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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