Rabbit Polyclonal SHANK3 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human SHANK3 aa 1450-1550.
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Publications
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- Redox biology 58:1025372022Ablation of Shank3 alleviates cardiac dysfunction in aging mice by promoting CaMKII activation and Parkin-mediated mitophagy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYing Wang,Yuerong Xu,Wangang Guo,Yexian Fang,Lang Hu,Runze Wang,Ran Zhao,Dong Guo,Bingchao Qi,Gaotong Ren,Jun Ren,Yan Li,Mingming ZhangPubMed 36436456
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: Tris citrate/phosphate- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human SHANK3 aa 1450-1550. The exact immunogen used to generate this antibody is proprietary information. Database link Q9BYB0
Reactivity data
Select an application| IP | WB | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Expected | Tested |
| Rat | Predicted | Predicted |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2.00000-10.00000 µg/mg of lysate | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/2000.00000 - 1/10000.00000 | Notes - |
Species Mouse | Dilution info 1/2000.00000 - 1/10000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
Associated Products
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2 products for Alternative Product
Target data
Function
Major scaffold postsynaptic density protein which interacts with multiple proteins and complexes to orchestrate the dendritic spine and synapse formation, maturation and maintenance. Interconnects receptors of the postsynaptic membrane including NMDA-type and metabotropic glutamate receptors via complexes with GKAP/PSD-95 and HOMER, respectively, and the actin-based cytoskeleton. Plays a role in the structural and functional organization of the dendritic spine and synaptic junction through the interaction with Arp2/3 and WAVE1 complex as well as the promotion of the F-actin clusters. By way of this control of actin dynamics, participates in the regulation of developing neurons growth cone motility and the NMDA receptor-signaling. Also modulates GRIA1 exocytosis and GRM5/MGLUR5 expression and signaling to control the AMPA and metabotropic glutamate receptor-mediated synaptic transmission and plasticity. May be required at an early stage of synapse formation and be inhibited by IGF1 to promote synapse maturation.
Alternative names
KIAA1650, PROSAP2, PSAP2, SHANK3, SH3 and multiple ankyrin repeat domains protein 3, Shank3, Proline-rich synapse-associated protein 2, ProSAP2
Recommended products
Rabbit Polyclonal SHANK3 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human SHANK3 aa 1450-1550.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: Tris citrate/phosphate- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human SHANK3 aa 1450-1550. The exact immunogen used to generate this antibody is proprietary information. Database link Q9BYB0
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The SHANK3 protein also known as ProSAP2 is a scaffold protein that plays an important role at the postsynaptic density of excitatory synapses. It has a mass of around 240 kDa. SHANK3 is expressed mainly in the brain but also present in heart spleen and kidneys. It interacts with various proteins through its multiple domains contributing to the structural integrity and signaling functions of synapses.
- Biological function summary
SHANK3 connects the cytoskeleton to the membrane proteins maintaining synaptic structure and function. As a part of the postsynaptic density complex SHANK3 interacts with proteins like Homer and NMDA receptors to organize the signaling platforms. Its role in synaptic structure regulation influences neuronal connectivity which impacts brain function and development.
- Pathways
SHANK3 interacts with the signaling cascade involved in synaptic plasticity and communication. Specifically it is important in the mTOR pathway which influences cell growth and synaptic protein synthesis and the glutamatergic synapse pathway essential for synaptic transmission. Within these pathways SHANK3 closely associates with proteins such as PSD-95 and SAPAP facilitating synaptic signal transduction.
- Associated diseases and disorders
SHANK3 mutations link to neurodevelopmental disorders including autism spectrum disorder (ASD) and Phelan-McDermid syndrome. These mutations can disrupt its interaction with proteins like PSD-95 affecting synaptic stability and leading to cognitive and social impairments. SHANK3's role in these conditions highlights the importance of proper synaptic signaling and structure for normal neurological function.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
Western blot - Anti-SHANK3 antibody (ab264347)
All lanes: Western blot - Anti-SHANK3 antibody (ab264347) at 0.1 µg/mL
Lane 1: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2: HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Lane 3: Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg
Lane 4: TCMK-1 (mouse kidney epithelial cell line) whole cell lysate at 50 µg
Lane 5: NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 186 kDa
Exposure time: 30s
Immunoprecipitation - Anti-SHANK3 antibody (ab264347)
SHANK3 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) using ab264347 at 6 μg/mg lysate. Western blot was performed on the immunoprecipitates using ab264347 at 1 μg/ml.
Lane 1: ab264347 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in Hela whole cell lysate.
Detection: Chemiluminescence with an exposure time of 10 seconds.
All lanes: Immunoprecipitation - Anti-SHANK3 antibody (ab264347)
Predicted band size: 186 kDa
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