AB93607

Anti-SHANK3 antibody [S69]

Name: Anti-SHANK3 antibody [S69] (ab93607) | Abcam
Brand: Abcam Limited
SKU: AB93607
Price: 540 USD
Availability: InStock
Rating: 3 (1 reviews)

(1 Review)

(5 Publications)

Mouse Monoclonal SHANK3 antibody. Suitable for WB, IHC-P and reacts with Rat, Mouse samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Rat Shank3 aa 800-900.

View Alternative Names

KIAA1650, PROSAP2, PSAP2, SHANK3, SH3 and multiple ankyrin repeat domains protein 3, Shank3, Proline-rich synapse-associated protein 2, ProSAP2

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHANK3 antibody [S69] (AB93607)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHANK3 antibody [S69] (AB93607)

Immunohistochemical analysis of mouse backskin tissue sections labling SHANK3 with ab93607 at 1/100 dilution. FITC Goat anti-mouse at 1/50 was used as the secondary antibody.

Supplier Data

Western blot - Anti-SHANK3 antibody [S69] (AB93607)

All lanes:

Western blot - Anti-SHANK3 antibody [S69] (ab93607) at 1/1000 dilution

All lanes:

Rat brain membrane lysate at 15 µg

Secondary

All lanes:

Sheep Anti-Mouse IgG-HRP

Predicted band size: 186 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

S69

Isotype

IgG2b

Carrier free

Reacts with

Mouse, Rat, Human

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Rat Shank3 aa 800-900. The exact immunogen used to generate this antibody is proprietary information.

Q9JLU4

Specificity

No cross-reactivity with SHANK1 or SHANK2.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Horse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

The clone number has been updated from S69-46 to N69/46, both clone numbers name the same antibody clone.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Storage buffer

Preservative: 0.09% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The SHANK3 protein also known as ProSAP2 is a scaffold protein that plays an important role at the postsynaptic density of excitatory synapses. It has a mass of around 240 kDa. SHANK3 is expressed mainly in the brain but also present in heart spleen and kidneys. It interacts with various proteins through its multiple domains contributing to the structural integrity and signaling functions of synapses.

Biological function summary

SHANK3 connects the cytoskeleton to the membrane proteins maintaining synaptic structure and function. As a part of the postsynaptic density complex SHANK3 interacts with proteins like Homer and NMDA receptors to organize the signaling platforms. Its role in synaptic structure regulation influences neuronal connectivity which impacts brain function and development.

Pathways

SHANK3 interacts with the signaling cascade involved in synaptic plasticity and communication. Specifically it is important in the mTOR pathway which influences cell growth and synaptic protein synthesis and the glutamatergic synapse pathway essential for synaptic transmission. Within these pathways SHANK3 closely associates with proteins such as PSD-95 and SAPAP facilitating synaptic signal transduction.

SHANK3 mutations link to neurodevelopmental disorders including autism spectrum disorder (ASD) and Phelan-McDermid syndrome. These mutations can disrupt its interaction with proteins like PSD-95 affecting synaptic stability and leading to cognitive and social impairments. SHANK3's role in these conditions highlights the importance of proper synaptic signaling and structure for normal neurological function.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Major scaffold postsynaptic density protein which interacts with multiple proteins and complexes to orchestrate the dendritic spine and synapse formation, maturation and maintenance. Interconnects receptors of the postsynaptic membrane including NMDA-type and metabotropic glutamate receptors via complexes with GKAP/PSD-95 and HOMER, respectively, and the actin-based cytoskeleton. Plays a role in the structural and functional organization of the dendritic spine and synaptic junction through the interaction with Arp2/3 and WAVE1 complex as well as the promotion of the F-actin clusters. By way of this control of actin dynamics, participates in the regulation of developing neurons growth cone motility and the NMDA receptor-signaling. Also modulates GRIA1 exocytosis and GRM5/MGLUR5 expression and signaling to control the AMPA and metabotropic glutamate receptor-mediated synaptic transmission and plasticity. May be required at an early stage of synapse formation and be inhibited by IGF1 to promote synapse maturation.

See full target information SHANK3

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Heliyon 9:e14328 PubMed36938421

2023

Role of SHANK3 in concentrated ambient PM2. 5 exposure induced autism-like phenotype.

Applications

Unspecified application

Species

Unspecified reactive species

Kang Li,Xiaotian Liang,Xiaoqian Xie,Lei Tian,Jun Yan,Bencheng Lin,Huanliang Liu,Wenqin Lai,Xiaohua Liu,Zhuge Xi

Cell death & disease 13:403 PubMed35468874

2022

SHANK1 facilitates non-small cell lung cancer processes through modulating the ubiquitination of Klotho by interacting with MDM2.

Applications

Unspecified application

Species

Unspecified reactive species

Bo Chen,Hongye Zhao,Min Li,Quan She,Wen Liu,Jiayi Zhang,Weihong Zhao,Shuhong Huang,Jianqing Wu

Frontiers in physiology 11:1082 PubMed32982797

2020

SHANK3 Co-ordinately Regulates Autophagy and Apoptosis in Myocardial Infarction.

Applications

Unspecified application

Species

Unspecified reactive species

Wanrong Man,Jing Gu,Bo Wang,Mingming Zhang,Jianqiang Hu,Jie Lin,Dong Sun,Zhenyu Xiong,Xiaoming Gu,Kaikai Hao,Baolin Guo,Gaoli Wei,Liang Zhang,Rui Song,Congye Li,Haichang Wang,Dongdong Sun

International journal of molecular sciences 21: PubMed32443651

2020

Prenatal Exposure to Valproic Acid Affects Microglia and Synaptic Ultrastructure in a Brain-Region-Specific Manner in Young-Adult Male Rats: Relevance to Autism Spectrum Disorders.

Applications

Unspecified application

Species

Unspecified reactive species

Magdalena Gąssowska-Dobrowolska,Magdalena Cieślik,Grzegorz Arkadiusz Czapski,Henryk Jęśko,Małgorzata Frontczak-Baniewicz,Magdalena Gewartowska,Agnieszka Dominiak,Rafał Polowy,Robert Kuba Filipkowski,Lidia Babiec,Agata Adamczyk

Experimental and therapeutic medicine 16:2375-2380 PubMed30186480

2018

Effect of intraperitoneal or intracerebroventricular injection of streptozotocin on learning and memory in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Zhang,Rong Ding,Shanshan Wang,Zhiyun Ren,Lingyan Xu,Xunle Zhang,Jiahui Zhao,Yu Ding,Yu Wu,Yuesong Gong

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SHANK3 antibody [S69]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHANK3 antibody [S69] (AB93607)

Western blot - Anti-SHANK3 antibody [S69] (AB93607)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

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