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AB244508

Anti-SHARP1 antibody

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(1 Review)

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(0 Publication)

Rabbit Polyclonal SHARP1 antibody. Suitable for ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human BHLHE41 aa 300-400.

View Alternative Names

BHLHB3, DEC2, SHARP1, BHLHE41, Class E basic helix-loop-helix protein 41, bHLHe41, Class B basic helix-loop-helix protein 3, Differentially expressed in chondrocytes protein 2, Enhancer-of-split and hairy-related protein 1, bHLHb3, hDEC2, SHARP-1

1 Images
Immunocytochemistry/ Immunofluorescence - Anti-SHARP1 antibody (AB244508)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-SHARP1 antibody (AB244508)

PFA-fixed, Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells stained for SHARP1 (green) using ab244508 at 4 μg/ml in ICC/IF.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human BHLHE41 aa 300-400. The exact immunogen used to generate this antibody is proprietary information.

Q9C0J9

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SHARP1 also known as DEC2 or bHLHE41 functions as a transcription factor involved in regulation of the circadian rhythm. It belongs to the basic helix-loop-helix (bHLH) family of transcription factors and has a mass of approximately 30 kDa. SHARP1 shows expression in various tissues including the brain liver and skeletal muscle where it contributes to controlling the expression of clock and other genes involved in circadian regulation.
Biological function summary

SHARP1 regulates genes by competing for binding to E-box sequences on DNA often through forming complexes with CLOCK and BMAL1 proteins. This interaction inhibits transcriptional activation altering the rhythmic expression of core clock genes and related metabolic pathways. SHARP1's role extends beyond circadian rhythms influencing processes like cell differentiation and proliferation.

Pathways

SHARP1 plays roles in circadian and metabolic pathways. In the circadian pathway SHARP1 modulates the feedback loops involving PER and CRY proteins impacting the cycle of gene expression. In metabolic pathways SHARP1 links to glucose metabolism through interaction with PGC-1α a transcription coactivator that regulates energy metabolism.

SHARP1 impacts sleep disorders such as Familial Advanced Sleep Phase Syndrome (FASPS) and some metabolic diseases. The alteration of SHARP1 expression or function can disrupt the circadian rhythms contributing to these conditions. SHARP1 also connects with the PER2 protein in circadian rhythm disorders highlighting its potential role in these pathological states.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcriptional repressor involved in the regulation of the circadian rhythm by negatively regulating the activity of the clock genes and clock-controlled genes (PubMed : 11278948, PubMed : 14672706, PubMed : 15193144, PubMed : 15560782, PubMed : 18411297, PubMed : 19786558, PubMed : 25083013). Acts as the negative limb of a novel autoregulatory feedback loop (DEC loop) which differs from the one formed by the PER and CRY transcriptional repressors (PER/CRY loop). Both these loops are interlocked as it represses the expression of PER1 and in turn is repressed by PER1/2 and CRY1/2. Represses the activity of the circadian transcriptional activator : CLOCK-BMAL1 heterodimer by competing for the binding to E-box elements (5'-CACGTG-3') found within the promoters of its target genes (PubMed : 25083013). Negatively regulates its own expression and the expression of DBP and BHLHE41/DEC2. Acts as a corepressor of RXR and the RXR-LXR heterodimers and represses the ligand-induced RXRA/B/G, NR1H3/LXRA, NR1H4 and VDR transactivation activity. Inhibits HNF1A-mediated transactivation of CYP1A2, CYP2E1 AND CYP3A11 (By similarity).
See full target information BHLHE41
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