Rabbit Polyclonal SHARP1 antibody. Suitable for ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human BHLHE41 aa 300-400.
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Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human BHLHE41 aa 300-400. The exact immunogen used to generate this antibody is proprietary information. Database link Q9C0J9
Reactivity data
Select an application| ICC/IF | |
|---|---|
| Human | Tested |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.25000-2.00000 µg/mL | Notes Fixation/Permeabilization: PFA/Triton X-100. |
Target data
Function
Transcriptional repressor involved in the regulation of the circadian rhythm by negatively regulating the activity of the clock genes and clock-controlled genes (PubMed:11278948, PubMed:14672706, PubMed:15193144, PubMed:15560782, PubMed:18411297, PubMed:19786558, PubMed:25083013). Acts as the negative limb of a novel autoregulatory feedback loop (DEC loop) which differs from the one formed by the PER and CRY transcriptional repressors (PER/CRY loop). Both these loops are interlocked as it represses the expression of PER1 and in turn is repressed by PER1/2 and CRY1/2. Represses the activity of the circadian transcriptional activator: CLOCK-BMAL1 heterodimer by competing for the binding to E-box elements (5'-CACGTG-3') found within the promoters of its target genes (PubMed:25083013). Negatively regulates its own expression and the expression of DBP and BHLHE41/DEC2. Acts as a corepressor of RXR and the RXR-LXR heterodimers and represses the ligand-induced RXRA/B/G, NR1H3/LXRA, NR1H4 and VDR transactivation activity. Inhibits HNF1A-mediated transactivation of CYP1A2, CYP2E1 AND CYP3A11 (By similarity).
Alternative names
BHLHB3, DEC2, SHARP1, BHLHE41, Class E basic helix-loop-helix protein 41, bHLHe41, Class B basic helix-loop-helix protein 3, Differentially expressed in chondrocytes protein 2, Enhancer-of-split and hairy-related protein 1, bHLHb3, hDEC2, SHARP-1
Recommended products
Rabbit Polyclonal SHARP1 antibody. Suitable for ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human BHLHE41 aa 300-400.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human BHLHE41 aa 300-400. The exact immunogen used to generate this antibody is proprietary information. Database link Q9C0J9
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
SHARP1 also known as DEC2 or bHLHE41 functions as a transcription factor involved in regulation of the circadian rhythm. It belongs to the basic helix-loop-helix (bHLH) family of transcription factors and has a mass of approximately 30 kDa. SHARP1 shows expression in various tissues including the brain liver and skeletal muscle where it contributes to controlling the expression of clock and other genes involved in circadian regulation.
- Biological function summary
SHARP1 regulates genes by competing for binding to E-box sequences on DNA often through forming complexes with CLOCK and BMAL1 proteins. This interaction inhibits transcriptional activation altering the rhythmic expression of core clock genes and related metabolic pathways. SHARP1's role extends beyond circadian rhythms influencing processes like cell differentiation and proliferation.
- Pathways
SHARP1 plays roles in circadian and metabolic pathways. In the circadian pathway SHARP1 modulates the feedback loops involving PER and CRY proteins impacting the cycle of gene expression. In metabolic pathways SHARP1 links to glucose metabolism through interaction with PGC-1α a transcription coactivator that regulates energy metabolism.
- Associated diseases and disorders
SHARP1 impacts sleep disorders such as Familial Advanced Sleep Phase Syndrome (FASPS) and some metabolic diseases. The alteration of SHARP1 expression or function can disrupt the circadian rhythms contributing to these conditions. SHARP1 also connects with the PER2 protein in circadian rhythm disorders highlighting its potential role in these pathological states.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Immunocytochemistry/ Immunofluorescence - Anti-SHARP1 antibody (ab244508)
PFA-fixed, Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells stained for SHARP1 (green) using ab244508 at 4 μg/ml in ICC/IF.
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