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AB70723

Anti-SHARP2/DEC1 antibody

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(7 Publications)

Rabbit Polyclonal SHARP2/DEC1 antibody. Suitable for IP, WB, IHC-P and reacts with Human samples. Cited in 7 publications. Immunogen corresponding to Synthetic Peptide within Human BHLHE40.

View Alternative Names

BHLHB2, DEC1, SHARP2, STRA13, BHLHE40, Class E basic helix-loop-helix protein 40, bHLHe40, Class B basic helix-loop-helix protein 2, Differentially expressed in chondrocytes protein 1, Enhancer-of-split and hairy-related protein 2, Stimulated by retinoic acid gene 13 protein, bHLHb2, SHARP-2

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHARP2/DEC1 antibody (AB70723)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHARP2/DEC1 antibody (AB70723)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling SHARP2/DEC1 with ab70723 at 1/1000 (1μg/ml). Detection : DAB.

Immunoprecipitation - Anti-SHARP2/DEC1 antibody (AB70723)
  • IP

Unknown

Immunoprecipitation - Anti-SHARP2/DEC1 antibody (AB70723)

Detection of Human SHARP2/DEC1 by Immunoprecipitation in whole cell lysate from 293T cells (1 mg for IP, 20% of IP loaded), using ab70723 at 3 μg/mg lysate (Lane 1). Lane 2 represents rabbit IgG IP control. Subsequent Western blot detection of SHARP2/DEC1 was performed using ab70723 at 1 μg/ml. Chemiluminescence with exposure time of 30 seconds

All lanes:

Immunoprecipitation - Anti-SHARP2/DEC1 antibody (ab70723)

Predicted band size: 46 kDa

false

Western blot - Anti-SHARP2/DEC1 antibody (AB70723)
  • WB

Unknown

Western blot - Anti-SHARP2/DEC1 antibody (AB70723)

All lanes:

Western blot - Anti-SHARP2/DEC1 antibody (ab70723) at 0.1 µg/mL

All lanes:

Whole cell lysate from HeLa cells at 50 µg

Predicted band size: 46 kDa

Observed band size: 50 kDa,65 kDa

true

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB, IP

applications

Immunogen

Synthetic Peptide within Human BHLHE40. The exact immunogen used to generate this antibody is proprietary information.

O14503

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1-4 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000 - 1/15000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/2000", "IHCP-species-notes": "<p></p>" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab70723 was affinity purified using an epitope specific to SHARP2/DEC1 immobilized on solid support.
Storage buffer
pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: PBS, 1.815% Tris, 1.764% Sodium citrate
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SHARP2 also known as DEC1 or BHLHE40 is a basic helix-loop-helix transcription factor with a molecular weight of approximately 39 kDa. This protein primarily regulates gene expression by binding to E-box sequences in the promoter regions of target genes. SHARP2/DEC1 expression occurs in various tissues such as the brain heart and lungs indicating its involvement in diverse physiological processes. The protein functions as a repressor and modulator of different genes linked to development and cellular responses to environmental changes.
Biological function summary

This transcription factor plays a significant role in the regulation of circadian rhythms and cell cycle progression. SHARP2/DEC1 interacts with various proteins to form complexes that influence cell differentiation and proliferation showing its critical function in maintaining cellular homeostasis. The protein also participates in hypoxia response being upregulated under low oxygen conditions which points to its adaptability and function under stress.

Pathways

SHARP2/DEC1 is an important component of the circadian clock and hypoxia signaling pathways. It modulates the activity of other clock genes and interacts with the PER and CRY protein families adding precision to circadian regulation. In the hypoxia pathway SHARP2/DEC1 activity affects the expression of genes involved in cellular adaptation to low oxygen levels cooperating with hypoxia-inducible factors to maintain balance during such conditions.

Changes in SHARP2/DEC1 expression link to cancer and metabolic syndromes. Elevated levels of this protein associate with aggressive tumor growth in different cancers highlighting its potential as a biomarker for tumor progression. Moreover SHARP2/DEC1 involvement in metabolic pathways shows its connection to disorders like obesity and diabetes. This protein's interaction with p53 in cancer pathways further portrays its influence on cell survival and apoptosis making it a significant target for therapeutic research.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcriptional repressor involved in the regulation of the circadian rhythm by negatively regulating the activity of the clock genes and clock-controlled genes (PubMed : 12397359, PubMed : 18411297). Acts as the negative limb of a novel autoregulatory feedback loop (DEC loop) which differs from the one formed by the PER and CRY transcriptional repressors (PER/CRY loop) (PubMed : 14672706). Both these loops are interlocked as it represses the expression of PER1/2 and in turn is repressed by PER1/2 and CRY1/2 (PubMed : 15193144). Represses the activity of the circadian transcriptional activator : CLOCK-BMAL1|BMAL2 heterodimer by competing for the binding to E-box elements (5'-CACGTG-3') found within the promoters of its target genes (PubMed : 15560782). Negatively regulates its own expression and the expression of DBP and BHLHE41/DEC2 (PubMed : 14672706). Acts as a corepressor of RXR and the RXR-LXR heterodimers and represses the ligand-induced RXRA and NR1H3/LXRA transactivation activity (PubMed : 19786558). May be involved in the regulation of chondrocyte differentiation via the cAMP pathway (PubMed : 19786558). Represses the transcription of NR0B2 and attentuates the transactivation of NR0B2 by the CLOCK-BMAL1 complex (PubMed : 28797635). Drives the circadian rhythm of blood pressure through transcriptional repression of ATP1B1 in the cardiovascular system (PubMed : 30012868).
See full target information BHLHE40
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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Hereditas 162:87 PubMed40413527

2025

BHLHE40-mediated RGS16 upregulation: a driver propelling gastric cancer progression via ferroptosis suppression.

Applications

Unspecified application

Species

Unspecified reactive species

Caiyun Guo,Hua Tang,Maifang Ren,Yongli Zhang

Cellular and molecular life sciences : CMLS 81:334 PubMed39115595

2024

IER3IP1-mutations cause microcephaly by selective inhibition of ER-Golgi transport.

Applications

Unspecified application

Species

Unspecified reactive species

Mihaela Anitei,Francesca Bruno,Christina Valkova,Therese Dau,Emilio Cirri,Iván Mestres,Federico Calegari,Christoph Kaether

Journal of gastrointestinal oncology 14:1504-1524 PubMed37435230

2023

A bioinformatics analysis and an experimental validation of the hypoxia-related prognostic model.

Applications

Unspecified application

Species

Unspecified reactive species

Lei Zhou,Weigang Zhang,Haoxiang Ni,Jin Liu,Hui Sun,Zhanwen Liang,Ruoqin Wang,Xiaofeng Xue,Kai Chen,Wei Li

Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology 51:780-790 PubMed36053880

2022

Overexpression of DEC1 in the epithelium of OSF promotes mesenchymal transition via activating FAK/Akt signal axis.

Applications

Unspecified application

Species

Unspecified reactive species

Xin Hu,Weiming Wang,Yue Hu,Wenxin Chen,Can Wang,Liudi Yang,Ting Mao,Kun Xia,Anjie Min,Haofeng Xiong,Tong Su

American journal of translational research 13:2331-2349 PubMed34017393

2021

Differentiated embryonic chondrocyte-expressed gene 1 promotes temozolomide resistance by modulating the SP1-MGMT axis in glioblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Weifeng Lv,Qi Li,Bo Jia,Yalong He,Yi Ru,Qingdong Guo,Xia Li,Wei Lin

Brazilian oral research 34:e052 PubMed32578762

2020

DEC1: a potential biomarker of malignant transformation in oral leukoplakia.

Applications

Unspecified application

Species

Unspecified reactive species

Ting Mao,Haofeng Xiong,Xin Hu,Yue Hu,Can Wang,Liudi Yang,Danni Huang,Kun Xia,Tong Su

International journal of molecular sciences 20: PubMed31575031

2019

iPSC-Derived Embryoid Bodies as Models of c--Mutated Hereditary Papillary Renal Cell Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Jin Wook Hwang,Christophe Desterke,Olivier Féraud,Stephane Richard,Sophie Ferlicot,Virginie Verkarre,Jean Jacques Patard,Julien Loisel-Duwattez,Adlen Foudi,Frank Griscelli,Annelise Bennaceur-Griscelli,Ali G Turhan
View all publications
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Product promise

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