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AB282588

Anti-SHARP2/DEC1 antibody [EPR23905-116] - BSA and Azide free

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Rabbit Recombinant Monoclonal SHARP2/DEC1 antibody. Carrier free. Suitable for WB and reacts with Human samples.

View Alternative Names

BHLHB2, DEC1, SHARP2, STRA13, BHLHE40, Class E basic helix-loop-helix protein 40, bHLHe40, Class B basic helix-loop-helix protein 2, Differentially expressed in chondrocytes protein 1, Enhancer-of-split and hairy-related protein 2, Stimulated by retinoic acid gene 13 protein, bHLHb2, SHARP-2

2 Images
Western blot - Anti-SHARP2/DEC1 antibody [EPR23905-116] - BSA and Azide free (AB282588)
  • WB

Lab

Western blot - Anti-SHARP2/DEC1 antibody [EPR23905-116] - BSA and Azide free (AB282588)

This data was developed using 259837, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST

Exposure time : 125 seconds

All lanes:

Western blot - Anti-SHARP2/DEC1 antibody [EPR23905-116] (<a href='/en-us/products/primary-antibodies/sharp2-dec1-antibody-epr23905-116-ab259837'>ab259837</a>) at 1/1000 dilution

Lane 1:

MCF7 (human breast adenocarcinoma epithelial cell) cytoplasmic fraction at 20 µg

Lane 2:

MCF7 (human breast adenocarcinoma epithelial cell) nuclear fraction at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 46 kDa

false

Western blot - Anti-SHARP2/DEC1 antibody [EPR23905-116] - BSA and Azide free (AB282588)
  • WB

Lab

Western blot - Anti-SHARP2/DEC1 antibody [EPR23905-116] - BSA and Azide free (AB282588)

This data was developed using 259837, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST

Exposure time : 3 minutes

All lanes:

Western blot - Anti-SHARP2/DEC1 antibody [EPR23905-116] (<a href='/en-us/products/primary-antibodies/sharp2-dec1-antibody-epr23905-116-ab259837'>ab259837</a>) at 1/1000 dilution

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma), whole cell lysate at 20 µg

Lane 2:

293T (human embryonic kidney epithelial cell), whole cell lysate at 20 µg

Lane 3:

MDA-MB-231 (human breast adenocarcinoma epithelial cell), whole cell lysate at 20 µg

Lane 4:

T47D (human ductal breast epithelial tumor epithelial cell), whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 46 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR23905-116

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Complementary Products

Secondary Antibodies

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Primary Antibodies

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BOND RX™ Validated|KO Validated

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Primary Antibodies

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Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker

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Primary Antibodies

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Reactivity data

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Product details

ab282588 is the carrier-free version of ab259837.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SHARP2 also known as DEC1 or BHLHE40 is a basic helix-loop-helix transcription factor with a molecular weight of approximately 39 kDa. This protein primarily regulates gene expression by binding to E-box sequences in the promoter regions of target genes. SHARP2/DEC1 expression occurs in various tissues such as the brain heart and lungs indicating its involvement in diverse physiological processes. The protein functions as a repressor and modulator of different genes linked to development and cellular responses to environmental changes.
Biological function summary

This transcription factor plays a significant role in the regulation of circadian rhythms and cell cycle progression. SHARP2/DEC1 interacts with various proteins to form complexes that influence cell differentiation and proliferation showing its critical function in maintaining cellular homeostasis. The protein also participates in hypoxia response being upregulated under low oxygen conditions which points to its adaptability and function under stress.

Pathways

SHARP2/DEC1 is an important component of the circadian clock and hypoxia signaling pathways. It modulates the activity of other clock genes and interacts with the PER and CRY protein families adding precision to circadian regulation. In the hypoxia pathway SHARP2/DEC1 activity affects the expression of genes involved in cellular adaptation to low oxygen levels cooperating with hypoxia-inducible factors to maintain balance during such conditions.

Changes in SHARP2/DEC1 expression link to cancer and metabolic syndromes. Elevated levels of this protein associate with aggressive tumor growth in different cancers highlighting its potential as a biomarker for tumor progression. Moreover SHARP2/DEC1 involvement in metabolic pathways shows its connection to disorders like obesity and diabetes. This protein's interaction with p53 in cancer pathways further portrays its influence on cell survival and apoptosis making it a significant target for therapeutic research.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcriptional repressor involved in the regulation of the circadian rhythm by negatively regulating the activity of the clock genes and clock-controlled genes (PubMed : 12397359, PubMed : 18411297). Acts as the negative limb of a novel autoregulatory feedback loop (DEC loop) which differs from the one formed by the PER and CRY transcriptional repressors (PER/CRY loop) (PubMed : 14672706). Both these loops are interlocked as it represses the expression of PER1/2 and in turn is repressed by PER1/2 and CRY1/2 (PubMed : 15193144). Represses the activity of the circadian transcriptional activator : CLOCK-BMAL1|BMAL2 heterodimer by competing for the binding to E-box elements (5'-CACGTG-3') found within the promoters of its target genes (PubMed : 15560782). Negatively regulates its own expression and the expression of DBP and BHLHE41/DEC2 (PubMed : 14672706). Acts as a corepressor of RXR and the RXR-LXR heterodimers and represses the ligand-induced RXRA and NR1H3/LXRA transactivation activity (PubMed : 19786558). May be involved in the regulation of chondrocyte differentiation via the cAMP pathway (PubMed : 19786558). Represses the transcription of NR0B2 and attentuates the transactivation of NR0B2 by the CLOCK-BMAL1 complex (PubMed : 28797635). Drives the circadian rhythm of blood pressure through transcriptional repression of ATP1B1 in the cardiovascular system (PubMed : 30012868).
See full target information BHLHE40
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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