Rabbit Polyclonal SHARPIN antibody. Suitable for IP, Flow Cyt, WB, ICC/IF and reacts with Human, Mouse samples. Cited in 6 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SHARPIN aa 1-250.
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- Cancer science 113:3766-37752022SHARPIN promotes cell proliferation of cholangiocarcinoma and inhibits ferroptosis via p53/SLC7A11/GPX4 signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChong Zeng,Jie Lin,Ketao Zhang,Huohui Ou,Ke Shen,Qingbo Liu,Zibo Wei,Xinhuai Dong,Xiaokang Zeng,Liming Zeng,Weidong Wang,Jie YaoPubMed 35968603
- Proceedings of the National Academy of Sciences of the United States of America 119:e21230971192022Reciprocal interplay between OTULIN-LUBAC determines genotoxic and inflammatory NF-κB signal responses.Applications:WBReactive species:MouseMingqi Li,Ling Li,Sarah Asemota,David Kakhniashvili,Ramesh Narayanan,Xusheng Wang,Francesca-Fang LiaoPubMed 35939695
- Frontiers in immunology 12:7497942021MALT1-Dependent Cleavage of HOIL1 Modulates Canonical NF-κB Signaling and Inflammatory Responsiveness.Applications:WBReactive species:HumanShan-Yu Fung,Henry Y Lu,Mehul Sharma,Ashish A Sharma,Aabida Saferali,Alicia Jia,Libin Abraham,Theo Klein,Michael R Gold,Luigi D Noterangelo,Christopher M Overall,Stuart E TurveyPubMed 34721419
- Molecular medicine reports 21:1799-18082020SHARPIN regulates cell proliferation of cutaneous basal cell carcinoma via inactivation of the transcriptional factors GLI2 and c‑JUN.
- Experimental dermatology 27:1328-13352018Down-regulated SHARPIN may accelerate the development of atopic dermatitis through activating interleukin-33/ST2 signalling.Applications:WBReactive species:HumanLingjie Tang,Jiaman Wang,Jingna Zhu,Yanhua LiangPubMed 30230040
- Oncotarget 8:54809-548202017A novel SHARPIN-PRMT5-H3R2me1 axis is essential for lung cancer cell invasion.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.05% Sodium azide
- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human SHARPIN aa 1-250. The exact immunogen used to generate this antibody is proprietary information. Database link Q9H0F6
Reactivity data
Select an application| IP | Flow Cyt | WB | ICC/IF | |
|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested |
| Mouse | Expected | Expected | Tested | Expected |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/200 | Notes ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/2000 | Notes - |
Species Human | Dilution info 1/2000 | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/2000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
3 products for Alternative Product
Target data
Function
Component of the LUBAC complex which conjugates linear polyubiquitin chains in a head-to-tail manner to substrates and plays a key role in NF-kappa-B activation and regulation of inflammation (PubMed:21455173, PubMed:21455180, PubMed:21455181). LUBAC conjugates linear polyubiquitin to IKBKG and RIPK1 and is involved in activation of the canonical NF-kappa-B and the JNK signaling pathways (PubMed:21455173, PubMed:21455180, PubMed:21455181). Linear ubiquitination mediated by the LUBAC complex interferes with TNF-induced cell death and thereby prevents inflammation (PubMed:21455173, PubMed:21455180, PubMed:21455181). LUBAC is recruited to the TNF-R1 signaling complex (TNF-RSC) following polyubiquitination of TNF-RSC components by BIRC2 and/or BIRC3 and to conjugate linear polyubiquitin to IKBKG and possibly other components contributing to the stability of the complex (PubMed:21455173, PubMed:21455180, PubMed:21455181). The LUBAC complex is also involved in innate immunity by conjugating linear polyubiquitin chains at the surface of bacteria invading the cytosol to form the ubiquitin coat surrounding bacteria (PubMed:28481331). LUBAC is not able to initiate formation of the bacterial ubiquitin coat, and can only promote formation of linear polyubiquitins on pre-existing ubiquitin (PubMed:28481331). The bacterial ubiquitin coat acts as an 'eat-me' signal for xenophagy and promotes NF-kappa-B activation (PubMed:28481331). Together with OTULIN, the LUBAC complex regulates the canonical Wnt signaling during angiogenesis (PubMed:23708998).
Alternative names
SIPL1, PSEC0216, SHARPIN, Sharpin, Shank-associated RH domain-interacting protein, Shank-interacting protein-like 1, hSIPL1
Recommended products
Rabbit Polyclonal SHARPIN antibody. Suitable for IP, Flow Cyt, WB, ICC/IF and reacts with Human, Mouse samples. Cited in 6 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SHARPIN aa 1-250.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.05% Sodium azide
- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human SHARPIN aa 1-250. The exact immunogen used to generate this antibody is proprietary information. Database link Q9H0F6
- Purity
- Whole antiserum
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
SHARPIN also known as SHANK-associated RH domain-interacting protein is a multi-functional protein with a molecular mass of approximately 34 kDa. It primarily acts as a ubiquitin-binding protein and is a part of the LUBAC (Linear Ubiquitin Chain Assembly Complex) involved in various cellular signaling pathways. SHARPIN is expressed in a range of tissues but is highly abundant in lymphoid tissues and the brain. Its mechanical actions involve binding and modulating ubiquitin chains which plays a role in regulating protein stability and signaling processes in cells.
- Biological function summary
SHARPIN engages in significant cellular functions by being a critical component of the LUBAC complex. This complex is central to the activation of NF-kB signaling by mediating linear ubiquitination of specific substrate proteins influencing inflammatory responses and cell death regulation. In the immune system SHARPIN regulates the TNF receptor signaling and helps maintain homeostasis. Its absence or mutation can lead to dysregulated cell survival and immune responses highlighting its importance in maintaining cellular functions.
- Pathways
SHARPIN integrates into the NF-kB and Wnt signaling pathways which are vital for regulating immune responses and cell fate decisions. Within the NF-kB pathway SHARPIN cooperates with other LUBAC components such as HOIP and HOIL-1L to facilitate ubiquitination necessary for pathway activation. Additionally SHARPIN influences the Wnt pathway through interactions with Dishevelled proteins contributing to developmental and stem cell signaling. The coordination of these pathways by SHARPIN highlights its role in cellular communication and signaling.
- Associated diseases and disorders
SHARPIN has connections to conditions such as chronic inflammation and immunodeficiency syndromes. Dysregulation in SHARPIN function can contribute to chronic inflammatory diseases including psoriasis due to improper NF-kB pathway regulation. Its association with HOIP and HOIL-1L through the LUBAC complex also links it to autoinflammatory conditions characterized by immune system imbalances. SHARPIN mutations are implicated in skin and immune disorders reflecting its broad impact on health and disease manifestation.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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4 product images
Immunoprecipitation - Anti-SHARPIN antibody (ab197853)
Western blot analysis with ab197853 of Immunoprecipitation
using U2OS cells extracts
Lane 1: Input.
Lane 2: Immunoprecipitation with control serum.
Lane 3: Immunoprecipitation with ab197853 (5μl)All lanes: Immunoprecipitation - Anti-SHARPIN antibody (ab197853)
Predicted band size: 23 kDa, 40 kDa
Western blot - Anti-SHARPIN antibody (ab197853)
Transfer in nitrocelullose, block: 1 hour 5% milk in TBST, incubation with primary antibody o/n @ 4oC in 5% milk TBST (0.1% Tween).
All lanes: Western blot - Anti-SHARPIN antibody (ab197853) at 1/2000 dilution
Lane 1: Control siRNA transfected U2OS cell lysate at 40 µg
Lane 2: Sharpin siRNA transfected U2OS cell lysate at 40 µg
Lane 3: NIH3T3 cell lysate at 40 µg
Predicted band size: 40 kDa
Flow Cytometry - Anti-SHARPIN antibody (ab197853)
Flow cytometric analysis of U2OS cells labeling Sharpin with ab197853 at 1/200 dilution. Upper panel shows labeling wit IgG controls, middle panel shows labeling of control siRNA treated cells and bottom panel shows labeling of Sharpin siRNA treated cells. Cells were fixed with 1% PFA. Cells were incubated with the primary antibody overnight at 4°C.
Immunocytochemistry/ Immunofluorescence - Anti-SHARPIN antibody (ab197853)
Immunofluorescent analysis of PFA fixed and methanol permeabilizaed U2OS cells transfected with HA-Sharpin labeling Sharpin with ab197853 at 1/200 dilution in PBST (green) and HA in (red)
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