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AB197853

Anti-SHARPIN antibody

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(6 Publications)

Rabbit Polyclonal SHARPIN antibody. Suitable for IP, Flow Cyt, WB, ICC/IF and reacts with Human, Mouse samples. Cited in 6 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SHARPIN aa 1-250.

View Alternative Names

SIPL1, PSEC0216, SHARPIN, Sharpin, Shank-associated RH domain-interacting protein, Shank-interacting protein-like 1, hSIPL1

4 Images
Immunoprecipitation - Anti-SHARPIN antibody (AB197853)
  • IP

Supplier Data

Immunoprecipitation - Anti-SHARPIN antibody (AB197853)

Western blot analysis with ab197853 of Immunoprecipitation
using U2OS cells extracts
Lane 1 : Input.
Lane 2 : Immunoprecipitation with control serum.
Lane 3 : Immunoprecipitation with ab197853 (5μl)

All lanes:

Immunoprecipitation - Anti-SHARPIN antibody (ab197853)

Predicted band size: 23 kDa,40 kDa

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Western blot - Anti-SHARPIN antibody (AB197853)
  • WB

Supplier Data

Western blot - Anti-SHARPIN antibody (AB197853)

Transfer in nitrocelullose, block : 1 hour 5% milk in TBST, incubation with primary antibody o/n @ 4oC in 5% milk TBST (0.1% Tween).

All lanes:

Western blot - Anti-SHARPIN antibody (ab197853) at 1/2000 dilution

Lane 1:

Control siRNA transfected U2OS cell lysate at 40 µg

Lane 2:

Sharpin siRNA transfected U2OS cell lysate at 40 µg

Lane 3:

NIH3T3 cell lysate at 40 µg

Predicted band size: 40 kDa

false

Flow Cytometry - Anti-SHARPIN antibody (AB197853)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-SHARPIN antibody (AB197853)

Flow cytometric analysis of U2OS cells labeling Sharpin with ab197853 at 1/200 dilution. Upper panel shows labeling wit IgG controls, middle panel shows labeling of control siRNA treated cells and bottom panel shows labeling of Sharpin siRNA treated cells. Cells were fixed with 1% PFA. Cells were incubated with the primary antibody overnight at 4°C.

Immunocytochemistry/ Immunofluorescence - Anti-SHARPIN antibody (AB197853)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SHARPIN antibody (AB197853)

Immunofluorescent analysis of PFA fixed and methanol permeabilizaed U2OS cells transfected with HA-Sharpin labeling Sharpin with ab197853 at 1/200 dilution in PBST (green) and HA in (red)

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

ICC/IF, WB, IP, Flow Cyt

applications

Immunogen

Recombinant Fragment Protein within Human SHARPIN aa 1-250. The exact immunogen used to generate this antibody is proprietary information.

Q9H0F6

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/200", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-polyclonal-isotype-control-chip-grade-ab171870'>ab171870</a> - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.</p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/2000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purity
Whole antiserum
Storage buffer
Preservative: 0.05% Sodium azide
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SHARPIN also known as SHANK-associated RH domain-interacting protein is a multi-functional protein with a molecular mass of approximately 34 kDa. It primarily acts as a ubiquitin-binding protein and is a part of the LUBAC (Linear Ubiquitin Chain Assembly Complex) involved in various cellular signaling pathways. SHARPIN is expressed in a range of tissues but is highly abundant in lymphoid tissues and the brain. Its mechanical actions involve binding and modulating ubiquitin chains which plays a role in regulating protein stability and signaling processes in cells.
Biological function summary

SHARPIN engages in significant cellular functions by being a critical component of the LUBAC complex. This complex is central to the activation of NF-kB signaling by mediating linear ubiquitination of specific substrate proteins influencing inflammatory responses and cell death regulation. In the immune system SHARPIN regulates the TNF receptor signaling and helps maintain homeostasis. Its absence or mutation can lead to dysregulated cell survival and immune responses highlighting its importance in maintaining cellular functions.

Pathways

SHARPIN integrates into the NF-kB and Wnt signaling pathways which are vital for regulating immune responses and cell fate decisions. Within the NF-kB pathway SHARPIN cooperates with other LUBAC components such as HOIP and HOIL-1L to facilitate ubiquitination necessary for pathway activation. Additionally SHARPIN influences the Wnt pathway through interactions with Dishevelled proteins contributing to developmental and stem cell signaling. The coordination of these pathways by SHARPIN highlights its role in cellular communication and signaling.

SHARPIN has connections to conditions such as chronic inflammation and immunodeficiency syndromes. Dysregulation in SHARPIN function can contribute to chronic inflammatory diseases including psoriasis due to improper NF-kB pathway regulation. Its association with HOIP and HOIL-1L through the LUBAC complex also links it to autoinflammatory conditions characterized by immune system imbalances. SHARPIN mutations are implicated in skin and immune disorders reflecting its broad impact on health and disease manifestation.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the LUBAC complex which conjugates linear polyubiquitin chains in a head-to-tail manner to substrates and plays a key role in NF-kappa-B activation and regulation of inflammation (PubMed : 21455173, PubMed : 21455180, PubMed : 21455181). LUBAC conjugates linear polyubiquitin to IKBKG and RIPK1 and is involved in activation of the canonical NF-kappa-B and the JNK signaling pathways (PubMed : 21455173, PubMed : 21455180, PubMed : 21455181). Linear ubiquitination mediated by the LUBAC complex interferes with TNF-induced cell death and thereby prevents inflammation (PubMed : 21455173, PubMed : 21455180, PubMed : 21455181). LUBAC is recruited to the TNF-R1 signaling complex (TNF-RSC) following polyubiquitination of TNF-RSC components by BIRC2 and/or BIRC3 and to conjugate linear polyubiquitin to IKBKG and possibly other components contributing to the stability of the complex (PubMed : 21455173, PubMed : 21455180, PubMed : 21455181). The LUBAC complex is also involved in innate immunity by conjugating linear polyubiquitin chains at the surface of bacteria invading the cytosol to form the ubiquitin coat surrounding bacteria (PubMed : 28481331). LUBAC is not able to initiate formation of the bacterial ubiquitin coat, and can only promote formation of linear polyubiquitins on pre-existing ubiquitin (PubMed : 28481331). The bacterial ubiquitin coat acts as an 'eat-me' signal for xenophagy and promotes NF-kappa-B activation (PubMed : 28481331). Together with OTULIN, the LUBAC complex regulates the canonical Wnt signaling during angiogenesis (PubMed : 23708998).
See full target information SHARPIN
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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Cancer science 113:3766-3775 PubMed35968603

2022

SHARPIN promotes cell proliferation of cholangiocarcinoma and inhibits ferroptosis via p53/SLC7A11/GPX4 signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Chong Zeng,Jie Lin,Ketao Zhang,Huohui Ou,Ke Shen,Qingbo Liu,Zibo Wei,Xinhuai Dong,Xiaokang Zeng,Liming Zeng,Weidong Wang,Jie Yao

Proceedings of the National Academy of Sciences of the United States of America 119:e2123097119 PubMed35939695

2022

Reciprocal interplay between OTULIN-LUBAC determines genotoxic and inflammatory NF-κB signal responses.

Applications

WB

Species

Mouse

Mingqi Li,Ling Li,Sarah Asemota,David Kakhniashvili,Ramesh Narayanan,Xusheng Wang,Francesca-Fang Liao

Frontiers in immunology 12:749794 PubMed34721419

2021

MALT1-Dependent Cleavage of HOIL1 Modulates Canonical NF-κB Signaling and Inflammatory Responsiveness.

Applications

WB

Species

Human

Shan-Yu Fung,Henry Y Lu,Mehul Sharma,Ashish A Sharma,Aabida Saferali,Alicia Jia,Libin Abraham,Theo Klein,Michael R Gold,Luigi D Noterangelo,Christopher M Overall,Stuart E Turvey

Molecular medicine reports 21:1799-1808 PubMed32319607

2020

SHARPIN regulates cell proliferation of cutaneous basal cell carcinoma via inactivation of the transcriptional factors GLI2 and c‑JUN.

Applications

WB

Species

Human

Yao Yang,Yan-Hua Liang,Yan Zheng,Ling-Jie Tang,Si-Tong Zhou,Jing-Na Zhu

Experimental dermatology 27:1328-1335 PubMed30230040

2018

Down-regulated SHARPIN may accelerate the development of atopic dermatitis through activating interleukin-33/ST2 signalling.

Applications

WB

Species

Human

Lingjie Tang,Jiaman Wang,Jingna Zhu,Yanhua Liang

Oncotarget 8:54809-54820 PubMed28903384

2017

A novel SHARPIN-PRMT5-H3R2me1 axis is essential for lung cancer cell invasion.

Applications

WB

Species

Human

Tingxiong Fu,Xiuwei Lv,Qingzhi Kong,Changjing Yuan
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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