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AB308572

Anti-SHB antibody [HL1455]

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Rabbit Recombinant Monoclonal SHB antibody. Carrier free. Suitable for IHC-P, WB and reacts with Mouse, Human samples. Immunogen corresponding to Synthetic Peptide within Human SH2 domain-containing adapter protein B.

View Alternative Names

SH2 domain-containing adapter protein B, SHB

2 Images
Immunohistochemistry paraffin embedded sections - Anti-SHB antibody [HL1455] (AB308572)
  • IHC-P

Supplier Data

Immunohistochemistry paraffin embedded sections - Anti-SHB antibody [HL1455] (AB308572)

Immunohistochemical analysis of paraffin-embedded mouse kidney and intestine tissues labeling SHB protein with ab308572 at 1/100. Antigen retrieval was performed using Citrate buffer, pH 6.0, 15 min

Western blot - Anti-SHB antibody [HL1455] (AB308572)
  • WB

Supplier Data

Western blot - Anti-SHB antibody [HL1455] (AB308572)

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SHB antibody HL1455 (ab308572) diluted at 1/1000. A HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

All lanes:

Western blot - Anti-SHB antibody [HL1455] (ab308572) at 1/1000 dilution

Lane 1:

HL-60 cell lysate at 30 µg

Lane 2:

HeLa cell lysate at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 55.042 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL1455

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Human

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human SH2 domain-containing adapter protein B. The exact immunogen used to generate this antibody is proprietary information.

Q15464

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The SHB protein also known as Src homology 2 domain-containing adapter protein B has a molecular mass of approximately 67 kDa. It functions as an adaptor protein mediating interactions between various signaling molecules. SHB facilitates signal transduction by forming connections between different proteins which can affect several cellular processes. This protein is expressed in a wide range of tissues including the liver pancreas and brain indicating its importance in multiple physiological contexts.
Biological function summary

SHB plays a role in cellular responses to external stimuli and is involved in processes such as cell growth differentiation and apoptosis. It is a component of signal transduction pathways and works in conjunction with other signaling molecules to modulate these processes. SHB often forms complexes with other proteins allowing it to integrate signals from different pathways and coordinate complex cellular responses.

Pathways

SHB participates in the MAPK/ERK and PI3K/AKT signaling pathways. These pathways are important for regulating cell survival growth and metabolism. SHB interacts with proteins like Grb2 and Sos within the MAPK/ERK pathway modulating their activity and in turn influencing downstream signaling events. In the PI3K/AKT pathway SHB associates with and modulates the activity of important mediator proteins further illustrating its integrative role in cellular signaling networks.

The dysregulation of SHB has been implicated in cancer and diabetes. In cancer SHB influences tumor progression and metastasis through its interactions with proteins like Src and PI3K which are critical in cancer biology. In diabetes altered SHB signaling affects insulin secretion and glucose metabolism linking it to diabetic pathophysiology. Understanding SHB's interactions in these diseases could provide insights into potential therapeutic targets.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Adapter protein which regulates several signal transduction cascades by linking activated receptors to downstream signaling components. May play a role in angiogenesis by regulating FGFR1, VEGFR2 and PDGFR signaling. May also play a role in T-cell antigen receptor/TCR signaling, interleukin-2 signaling, apoptosis and neuronal cells differentiation by mediating basic-FGF and NGF-induced signaling cascades. May also regulate IRS1 and IRS2 signaling in insulin-producing cells.
See full target information SH2 domain-containing adapter protein B
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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