AB219575

Anti-SHBG antibody [EPR21758]

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal SHBG antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

Sex hormone-binding globulin, SHBG, Sex steroid-binding protein, Testis-specific androgen-binding protein, Testosterone-estradiol-binding globulin, Testosterone-estrogen-binding globulin, SBP, ABP, TeBG

2 Images

Supplier Data

Western blot - Anti-SHBG antibody [EPR21758] (AB219575)

Blocking/ Dilution buffer and concentration : 5% NFDM/TBST.

Negative control : MCF-7 (PMID : 8545857)

All lanes:

Western blot - Anti-SHBG antibody [EPR21758] (ab219575) at 1/1000 dilution

Lane 1:

HepG2 (human hepatocellular carcinoma epithelial cell) supernatant at 10 µL

Lane 2:

MCF7 (human breast adenocarcinoma epithelial cell) supernatant at 10 µL

Secondary

All lanes:

Western blot - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/500 dilution

Predicted band size: 44 kDa

Observed band size: 44 kDa

true

Exposure time: 3min

Supplier Data

Western blot - Anti-SHBG antibody [EPR21758] (AB219575)

Blocking/ Dilution buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-SHBG antibody [EPR21758] (ab219575) at 1/1000 dilution

Lane 1:

Human serum at 20 µg

Lane 2:

Human testis lysate at 10 µg

Secondary

Lane 1:

Western blot - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/1000 dilution

Lane 2:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 44 kDa

Observed band size: 44 kDa

true

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR21758

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein sex hormone-binding globulin (SHBG) also known as testosterone-estradiol binding globulin (TEBG) plays an important role in binding and transporting sex steroids in the bloodstream. This glycoprotein has a mass of approximately 90 kDa. SHBG is produced mainly in the liver but it is also expressed in other tissues such as the testes ovaries and placenta. It regulates the availability of sex hormones to tissues and influences their bioactivity at the cellular level.

Biological function summary

SHBG regulates the levels of circulating androgens and estrogens by binding tightly to testosterone and estradiol. This binding inhibits the hormones from interacting with their receptors until they are released therefore controlling their action. SHBG can exist as a monomer but often forms a homodimer complex. In addition to binding sex hormones SHBG also interacts with membrane receptors and may participate in the direct signaling pathways that modulate cellular responses.

Pathways

SHBG plays a role in the regulation of the steroid hormone pathway and is related to the transport and metabolic clearance of sex hormones. This pathway involves transformation and transportation of estrogens and androgens affecting genes regulated by these hormones. SHBG interacts with various proteins including albumin which assists in hormone transport and it might influence the androgen receptor pathway altering cell proliferation and differentiation signals.

SHBG levels influence conditions such as polycystic ovary syndrome (PCOS) and type 2 diabetes. In PCOS lower levels of SHBG can result in greater circulating free testosterone which may exacerbate symptoms. In the context of type 2 diabetes alterations in SHBG levels may affect insulin resistance possibly linked with insulin and glucose transport proteins. Understanding the dynamics of SHBG could aid in the development of therapeutic strategies targeting these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Functions as an androgen transport protein, but may also be involved in receptor mediated processes. Each dimer binds one molecule of steroid. Specific for 5-alpha-dihydrotestosterone, testosterone, and 17-beta-estradiol. Regulates the plasma metabolic clearance rate of steroid hormones by controlling their plasma concentration.

See full target information SHBG

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature cardiovascular research 4:145-162 PubMed39794479

2025

Endothelial IGFBP6 suppresses vascular inflammation and atherosclerosis.

Applications

Unspecified application

Species

Unspecified reactive species

Meiming Su,Wenqi Zhao,Hui Jiang,Yaping Zhao,Zhaopeng Liao,Zhenghong Liu,Mengyun Xu,Shanshan Jiang,Lili Wu,Yi Yang,Zhihua Wang,Zhutian Zeng,Yun Fang,Chaojun Tang,Clint L Miller,Paul C Evans,Li Wang,Maciej Banach,Hanjoong Jo,Bradford C Berk,Stefan Offermanns,Yu Huang,Junbo Ge,Suowen Xu,Jianping Weng

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SHBG antibody [EPR21758]

Western blot - Anti-SHBG antibody [EPR21758] (AB219575)

Western blot - Anti-SHBG antibody [EPR21758] (AB219575)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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