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AB222402

Anti-SHBG antibody [EPR6628] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal SHBG antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

Sex hormone-binding globulin, SHBG, Sex steroid-binding protein, Testis-specific androgen-binding protein, Testosterone-estradiol-binding globulin, Testosterone-estrogen-binding globulin, SBP, ABP, TeBG

2 Images
Western blot - Anti-SHBG antibody [EPR6628] - BSA and Azide free (AB222402)
  • WB

Lab

Western blot - Anti-SHBG antibody [EPR6628] - BSA and Azide free (AB222402)

This data was developed using ab126617, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-SHBG antibody [EPR6628] (<a href='/en-us/products/primary-antibodies/shbg-antibody-epr6628-ab126617'>ab126617</a>) at 1/1000 dilution

All lanes:

Human placenta tissue lysate at 10 µg

Secondary

All lanes:

HRP anti-rabbit IgG, specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 44 kDa

Observed band size: 44 kDa

false

Western blot - Anti-SHBG antibody [EPR6628] - BSA and Azide free (AB222402)
  • WB

Unknown

Western blot - Anti-SHBG antibody [EPR6628] - BSA and Azide free (AB222402)

This data was developed using ab126617, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-SHBG antibody [EPR6628] (<a href='/en-us/products/primary-antibodies/shbg-antibody-epr6628-ab126617'>ab126617</a>) at 1/1000 dilution

All lanes:

Human placenta tissue lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP-conjugated at 1/2000 dilution

Predicted band size: 44 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR6628

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Complementary Products

Assay Kits

AB260070

Human SHBG ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human SHBG ELISA Kit (AB260070)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB8227

Anti-beta Actin antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Western blot - Anti-beta Actin antibody - Loading Control (AB8227)

  • 5
  • 104
Primary Antibodies

AB33780

Anti-Glucose Transporter GLUT4 antibody

BOND RX™ Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT4 antibody (AB33780)

  • 4
  • 12
Primary Antibodies

AB191071

Anti-Glucose Transporter GLUT3 + GLUT14 antibody [EPR10508(N)] - N-terminal

RabMAb|Recombinant|Trial Size

Western blot - Anti-Glucose Transporter GLUT3 + GLUT14 antibody [EPR10508(N)] - N-terminal (AB191071)

  • 5
  • 6
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

ab222402 is the carrier-free version of ab126617.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein sex hormone-binding globulin (SHBG) also known as testosterone-estradiol binding globulin (TEBG) plays an important role in binding and transporting sex steroids in the bloodstream. This glycoprotein has a mass of approximately 90 kDa. SHBG is produced mainly in the liver but it is also expressed in other tissues such as the testes ovaries and placenta. It regulates the availability of sex hormones to tissues and influences their bioactivity at the cellular level.
Biological function summary

SHBG regulates the levels of circulating androgens and estrogens by binding tightly to testosterone and estradiol. This binding inhibits the hormones from interacting with their receptors until they are released therefore controlling their action. SHBG can exist as a monomer but often forms a homodimer complex. In addition to binding sex hormones SHBG also interacts with membrane receptors and may participate in the direct signaling pathways that modulate cellular responses.

Pathways

SHBG plays a role in the regulation of the steroid hormone pathway and is related to the transport and metabolic clearance of sex hormones. This pathway involves transformation and transportation of estrogens and androgens affecting genes regulated by these hormones. SHBG interacts with various proteins including albumin which assists in hormone transport and it might influence the androgen receptor pathway altering cell proliferation and differentiation signals.

SHBG levels influence conditions such as polycystic ovary syndrome (PCOS) and type 2 diabetes. In PCOS lower levels of SHBG can result in greater circulating free testosterone which may exacerbate symptoms. In the context of type 2 diabetes alterations in SHBG levels may affect insulin resistance possibly linked with insulin and glucose transport proteins. Understanding the dynamics of SHBG could aid in the development of therapeutic strategies targeting these conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Functions as an androgen transport protein, but may also be involved in receptor mediated processes. Each dimer binds one molecule of steroid. Specific for 5-alpha-dihydrotestosterone, testosterone, and 17-beta-estradiol. Regulates the plasma metabolic clearance rate of steroid hormones by controlling their plasma concentration.
See full target information SHBG
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Aging 12:2595-2609 PubMed32028262

2020

Notch1/2/3/4 are prognostic biomarker and correlated with immune infiltrates in gastric cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Jian Hu,Jianghong Yu,Jun Gan,Ning Song,Liubin Shi,Jie Liu,Ziqiang Zhang,Jianjun Du
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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