Rabbit Recombinant Monoclonal SHBG antibody. Carrier free. Suitable for WB and reacts with Human samples.
Images
![Western blot - Anti-SHBG antibody [EPR6628] - BSA and Azide free (AB222402), expandable thumbnail](https://content.abcam.com/products/images/shbg-antibody-epr6628-bsa-and-azide-free-ab222402--western-blot-img78550.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-SHBG antibody [EPR6628] - BSA and Azide free (AB222402)")
![Western blot - Anti-SHBG antibody [EPR6628] - BSA and Azide free (AB222402), expandable thumbnail](https://content.abcam.com/products/images/shbg-antibody-epr6628-bsa-and-azide-free-ab222402--western-blot-img81681.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-SHBG antibody [EPR6628] - BSA and Azide free (AB222402)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Human | Tested | Not recommended |
| Mouse | Predicted | Not recommended |
| Rat | Predicted | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Target data
Function
Functions as an androgen transport protein, but may also be involved in receptor mediated processes. Each dimer binds one molecule of steroid. Specific for 5-alpha-dihydrotestosterone, testosterone, and 17-beta-estradiol. Regulates the plasma metabolic clearance rate of steroid hormones by controlling their plasma concentration.
Alternative names
Sex hormone-binding globulin, SHBG, Sex steroid-binding protein, Testis-specific androgen-binding protein, Testosterone-estradiol-binding globulin, Testosterone-estrogen-binding globulin, SBP, ABP, TeBG
Recommended products
Rabbit Recombinant Monoclonal SHBG antibody. Carrier free. Suitable for WB and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR6628
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab222402 is the carrier-free version of Anti-SHBG antibody [EPR6628] ab126617.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The protein sex hormone-binding globulin (SHBG) also known as testosterone-estradiol binding globulin (TEBG) plays an important role in binding and transporting sex steroids in the bloodstream. This glycoprotein has a mass of approximately 90 kDa. SHBG is produced mainly in the liver but it is also expressed in other tissues such as the testes ovaries and placenta. It regulates the availability of sex hormones to tissues and influences their bioactivity at the cellular level.
- Biological function summary
SHBG regulates the levels of circulating androgens and estrogens by binding tightly to testosterone and estradiol. This binding inhibits the hormones from interacting with their receptors until they are released therefore controlling their action. SHBG can exist as a monomer but often forms a homodimer complex. In addition to binding sex hormones SHBG also interacts with membrane receptors and may participate in the direct signaling pathways that modulate cellular responses.
- Pathways
SHBG plays a role in the regulation of the steroid hormone pathway and is related to the transport and metabolic clearance of sex hormones. This pathway involves transformation and transportation of estrogens and androgens affecting genes regulated by these hormones. SHBG interacts with various proteins including albumin which assists in hormone transport and it might influence the androgen receptor pathway altering cell proliferation and differentiation signals.
- Associated diseases and disorders
SHBG levels influence conditions such as polycystic ovary syndrome (PCOS) and type 2 diabetes. In PCOS lower levels of SHBG can result in greater circulating free testosterone which may exacerbate symptoms. In the context of type 2 diabetes alterations in SHBG levels may affect insulin resistance possibly linked with insulin and glucose transport proteins. Understanding the dynamics of SHBG could aid in the development of therapeutic strategies targeting these conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Western blot - Anti-SHBG antibody [EPR6628] - BSA and Azide free (ab222402), expandable thumbnail](https://content.abcam.com/products/images/shbg-antibody-epr6628-bsa-and-azide-free-ab222402--western-blot-img78550.jpg "Western blot - Anti-SHBG antibody [EPR6628] - BSA and Azide free (ab222402)")
Western blot - Anti-SHBG antibody [EPR6628] - BSA and Azide free (ab222402)
This data was developed using Anti-SHBG antibody [EPR6628] ab126617, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-SHBG antibody [EPR6628] (Anti-SHBG antibody [EPR6628] ab126617) at 1/1000 dilution
All lanes: Human placenta tissue lysate at 10 µg
SecondaryAll lanes: HRP anti-rabbit IgG, specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 44 kDa
Observed band size: 44 kDa
![Western blot - Anti-SHBG antibody [EPR6628] - BSA and Azide free (ab222402), expandable thumbnail](https://content.abcam.com/products/images/shbg-antibody-epr6628-bsa-and-azide-free-ab222402--western-blot-img81681.jpg "Western blot - Anti-SHBG antibody [EPR6628] - BSA and Azide free (ab222402)")
Western blot - Anti-SHBG antibody [EPR6628] - BSA and Azide free (ab222402)
This data was developed using Anti-SHBG antibody [EPR6628] ab126617, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-SHBG antibody [EPR6628] (Anti-SHBG antibody [EPR6628] ab126617) at 1/1000 dilution
All lanes: Human placenta tissue lysate at 10 µg
SecondaryAll lanes: Goat anti-rabbit HRP-conjugated at 1/2000 dilution
Predicted band size: 44 kDa
Downloads
Product protocols
- Visit the general protocols
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