Rabbit Polyclonal SHC antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human SHC-transforming protein 1 aa 400-450 conjugated to Keyhole Limpet Haemocyanin.
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Publications
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- Nature cell biology 21:1553-15642019Diurnal oscillations of endogenous HO sustained by p66 regulate circadian clocks.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJian-Fei Pei et. al.PubMed 31768048
- Antioxidants & redox signaling 30:1949-19682019Isosteviol Protects Free Fatty Acid- and High Fat Diet-Induced Hepatic Injury Modulating PKC-β/p66Shc/ROS and Endoplasmic Reticulum Stress Pathways.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHongwei Yi et. al.PubMed 30484323
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 48% PBS, 0.88% Sodium chloride- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Synthetic Peptide within Human SHC-transforming protein 1 aa 400-450 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link P29353
Reactivity data
Select an application| WB | IHC-P | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Predicted | Predicted | Predicted |
| Rat | Predicted | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/1000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50.00000 - 1/100.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/200.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Associated Products
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Target data
Function
Signaling adapter that couples activated growth factor receptors to signaling pathways. Participates in a signaling cascade initiated by activated KIT and KITLG/SCF. Isoform p46Shc and isoform p52Shc, once phosphorylated, couple activated receptor tyrosine kinases to Ras via the recruitment of the GRB2/SOS complex and are implicated in the cytoplasmic propagation of mitogenic signals. Isoform p46Shc and isoform p52Shc may thus function as initiators of the Ras signaling cascade in various non-neuronal systems. Isoform p66Shc does not mediate Ras activation, but is involved in signal transduction pathways that regulate the cellular response to oxidative stress and life span. Isoform p66Shc acts as a downstream target of the tumor suppressor p53 and is indispensable for the ability of stress-activated p53 to induce elevation of intracellular oxidants, cytochrome c release and apoptosis. The expression of isoform p66Shc has been correlated with life span (By similarity). Participates in signaling downstream of the angiopoietin receptor TEK/TIE2, and plays a role in the regulation of endothelial cell migration and sprouting angiogenesis.
Alternative names
SHC, SHCA, SHC1, SHC, SHCA, SHC-transforming protein 1, SHC-transforming protein 3, SHC-transforming protein A, Src homology 2 domain-containing-transforming protein C1, SH2 domain protein C1
Recommended products
Rabbit Polyclonal SHC antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human SHC-transforming protein 1 aa 400-450 conjugated to Keyhole Limpet Haemocyanin.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 48% PBS, 0.88% Sodium chloride- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Synthetic Peptide within Human SHC-transforming protein 1 aa 400-450 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link P29353
- Purification technique
Affinity purification Immunogen
- Concentration
- Purification notes
ab131515 was purified by affinity chromatography using epitope specific peptide.
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Storage information
Stable for 12 months at -20°C
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
SHC also known as Src homology 2 domain-containing transforming protein or p52Shc is an adaptor protein involved in signal transduction pathways. It has a molecular mass of approximately 52 kDa. SHC contains an SH2 domain which facilitates its binding to tyrosine-phosphorylated activated receptors. This protein is ubiquitously expressed with notable levels in tissues like the brain heart and melanocytes. Its role as a signaling adaptor makes it an important component for cell communication and function.
- Biological function summary
The SHC protein transmits signals from cell surface receptors to downstream effectors. It does so by linking activated receptors to the Ras-MAPK signaling cascade. SHC often part of larger protein complexes interacts with proteins such as Grb2 and SOS to relay signals necessary for cell growth and differentiation. This interaction leads to the activation of Ras and subsequent mitogen-activated protein kinase pathways influencing various cellular responses.
- Pathways
SHC is a central component of the MAPK/ERK and PI3K/AKT pathways which are important for cell growth and survival. In these pathways SHC mediates signals from receptor tyrosine kinases like the epidermal growth factor receptor (EGFR). By interacting with EGFR and other receptors SHC helps propagate signals essential for cellular proliferation and survival. SHC associates with proteins like Grb2 and SOS linking RTKs to the Ras signaling cascade.
- Associated diseases and disorders
SHC has been linked to cancer and cardiovascular diseases. In cancer abnormal SHC-mediated signaling can result from overexpression or mutation leading to unregulated cell proliferation. This abnormal activity often involves interactions with proteins like EGFR and Grb2 which drive oncogenic pathways. Regarding cardiovascular diseases changes in SHC expression or function can contribute to conditions like hypertrophy where SHC-associated pathways influence heart muscle growth and stress responses.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Western blot - Anti-SHC antibody (ab131515)
All lanes: Western blot - Anti-SHC antibody (ab131515) at 1/500 dilution
All lanes: 293 cell extract
Predicted band size: 62 kDa
Immunocytochemistry/ Immunofluorescence - Anti-SHC antibody (ab131515)
Immunofluorescent analysis of methanol-fixed HeLa cells labelling SHC with ab131515 at 1/100 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHC antibody (ab131515)
Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labelling SHC with ab131515 at 1/50 dilution. The image on the right is treated with the synthesized peptide.
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