Rabbit Polyclonal SHC antibody. Suitable for WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human SHC1.
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- Frontiers in oncology 9:11202019Epstein-Barr Virus BART Long Non-coding RNAs Function as Epigenetic Modulators in Nasopharyngeal Carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRob J A Verhoeven et. al.PubMed 31696060
- Molecular and cellular biology 36:1509-252016Chordin-Like 1 Suppresses Bone Morphogenetic Protein 4-Induced Breast Cancer Cell Migration and Invasion.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChanèle Cyr-Depauw et. al.PubMed 26976638
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human SHC1. The exact immunogen used to generate this antibody is proprietary information. Database link P29353
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Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 | Notes - |
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Target data
Function
Signaling adapter that couples activated growth factor receptors to signaling pathways. Participates in a signaling cascade initiated by activated KIT and KITLG/SCF. Isoform p46Shc and isoform p52Shc, once phosphorylated, couple activated receptor tyrosine kinases to Ras via the recruitment of the GRB2/SOS complex and are implicated in the cytoplasmic propagation of mitogenic signals. Isoform p46Shc and isoform p52Shc may thus function as initiators of the Ras signaling cascade in various non-neuronal systems. Isoform p66Shc does not mediate Ras activation, but is involved in signal transduction pathways that regulate the cellular response to oxidative stress and life span. Isoform p66Shc acts as a downstream target of the tumor suppressor p53 and is indispensable for the ability of stress-activated p53 to induce elevation of intracellular oxidants, cytochrome c release and apoptosis. The expression of isoform p66Shc has been correlated with life span (By similarity). Participates in signaling downstream of the angiopoietin receptor TEK/TIE2, and plays a role in the regulation of endothelial cell migration and sprouting angiogenesis.
Alternative names
SHC, SHCA, SHC1, SHC-transforming protein 1, SHC-transforming protein 3, SHC-transforming protein A, Src homology 2 domain-containing-transforming protein C1, SH2 domain protein C1
Recommended products
Rabbit Polyclonal SHC antibody. Suitable for WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human SHC1.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human SHC1. The exact immunogen used to generate this antibody is proprietary information. Database link P29353
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
SHC also known as Src homology 2 domain-containing transforming protein or p52Shc is an adaptor protein involved in signal transduction pathways. It has a molecular mass of approximately 52 kDa. SHC contains an SH2 domain which facilitates its binding to tyrosine-phosphorylated activated receptors. This protein is ubiquitously expressed with notable levels in tissues like the brain heart and melanocytes. Its role as a signaling adaptor makes it an important component for cell communication and function.
- Biological function summary
The SHC protein transmits signals from cell surface receptors to downstream effectors. It does so by linking activated receptors to the Ras-MAPK signaling cascade. SHC often part of larger protein complexes interacts with proteins such as Grb2 and SOS to relay signals necessary for cell growth and differentiation. This interaction leads to the activation of Ras and subsequent mitogen-activated protein kinase pathways influencing various cellular responses.
- Pathways
SHC is a central component of the MAPK/ERK and PI3K/AKT pathways which are important for cell growth and survival. In these pathways SHC mediates signals from receptor tyrosine kinases like the epidermal growth factor receptor (EGFR). By interacting with EGFR and other receptors SHC helps propagate signals essential for cellular proliferation and survival. SHC associates with proteins like Grb2 and SOS linking RTKs to the Ras signaling cascade.
- Associated diseases and disorders
SHC has been linked to cancer and cardiovascular diseases. In cancer abnormal SHC-mediated signaling can result from overexpression or mutation leading to unregulated cell proliferation. This abnormal activity often involves interactions with proteins like EGFR and Grb2 which drive oncogenic pathways. Regarding cardiovascular diseases changes in SHC expression or function can contribute to conditions like hypertrophy where SHC-associated pathways influence heart muscle growth and stress responses.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Western blot - Anti-SHC antibody (ab24787)
Membrane was incubated with diluted antibody in 5% non fat milk, PBS, 0.04% Tween 20 for 1 hour at room temperature.
All lanes: Western blot - Anti-SHC antibody (ab24787) at 1/1000 dilution
Lane 1: A431 cell lysate at 20 µg
Lane 2: Jurkat cell lysate at 20 µg
Predicted band size: 62 kDa
Observed band size: 46 kDa, 52 kDa, 66 kDa
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