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AB247869

Anti-SHC (phospho Y239 + Y240) antibody [EP419] - BSA and Azide free

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Rabbit Recombinant Monoclonal SHC phospho Y239 antibody. Carrier free. Suitable for WB and reacts with Human samples.

View Alternative Names

SHC, SHCA, SHC1, SHC-transforming protein 1, SHC-transforming protein 3, SHC-transforming protein A, Src homology 2 domain-containing-transforming protein C1, SH2 domain protein C1

2 Images
Western blot - Anti-SHC (phospho Y239 + Y240) antibody [EP419] - BSA and Azide free (AB247869)
  • WB

Supplier Data

Western blot - Anti-SHC (phospho Y239 + Y240) antibody [EP419] - BSA and Azide free (AB247869)

This data was developed using ab109455, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-SHC (phospho Y239 + Y240) antibody [EP419] (<a href='/en-us/products/primary-antibodies/shc-phospho-y239-y240-antibody-ep419-ab109455'>ab109455</a>) at 1/5000 dilution

Lane 1:

293T cell lysates, EGF treated at 10 µg with single phospho (pY239) peptide

Lane 2:

293T cell lysates, EGF treated at 10 µg with single phospho (pY240) peptide

Lane 3:

293T cell lysates, EGF treated at 10 µg with Double phospho (pY239/pY240) peptide

Lane 4:

293T cell lysates, EGF treated at 10 µg with non-phospho peptide blocking

Lane 5:

293T cell lysates, EGF treated at 10 µg

Predicted band size: 62 kDa

false

Western blot - Anti-SHC (phospho Y239 + Y240) antibody [EP419] - BSA and Azide free (AB247869)
  • WB

Unknown

Western blot - Anti-SHC (phospho Y239 + Y240) antibody [EP419] - BSA and Azide free (AB247869)

This data was developed using ab109455, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-SHC (phospho Y239 + Y240) antibody [EP419] (<a href='/en-us/products/primary-antibodies/shc-phospho-y239-y240-antibody-ep419-ab109455'>ab109455</a>) at 1/500 dilution

Lane 1:

293T cell lysates, untreated at 10 µg

Lane 2:

293T cell lysates, treated with EGF at 10 µg

Predicted band size: 62 kDa

false

  • Unconjugated

    Anti-SHC (phospho Y239 + Y240) antibody [EP419]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP419

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab247869 is the carrier-free version of ab109455.

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SHC also known as Src homology 2 domain-containing transforming protein or p52Shc is an adaptor protein involved in signal transduction pathways. It has a molecular mass of approximately 52 kDa. SHC contains an SH2 domain which facilitates its binding to tyrosine-phosphorylated activated receptors. This protein is ubiquitously expressed with notable levels in tissues like the brain heart and melanocytes. Its role as a signaling adaptor makes it an important component for cell communication and function.
Biological function summary

The SHC protein transmits signals from cell surface receptors to downstream effectors. It does so by linking activated receptors to the Ras-MAPK signaling cascade. SHC often part of larger protein complexes interacts with proteins such as Grb2 and SOS to relay signals necessary for cell growth and differentiation. This interaction leads to the activation of Ras and subsequent mitogen-activated protein kinase pathways influencing various cellular responses.

Pathways

SHC is a central component of the MAPK/ERK and PI3K/AKT pathways which are important for cell growth and survival. In these pathways SHC mediates signals from receptor tyrosine kinases like the epidermal growth factor receptor (EGFR). By interacting with EGFR and other receptors SHC helps propagate signals essential for cellular proliferation and survival. SHC associates with proteins like Grb2 and SOS linking RTKs to the Ras signaling cascade.

SHC has been linked to cancer and cardiovascular diseases. In cancer abnormal SHC-mediated signaling can result from overexpression or mutation leading to unregulated cell proliferation. This abnormal activity often involves interactions with proteins like EGFR and Grb2 which drive oncogenic pathways. Regarding cardiovascular diseases changes in SHC expression or function can contribute to conditions like hypertrophy where SHC-associated pathways influence heart muscle growth and stress responses.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Signaling adapter that couples activated growth factor receptors to signaling pathways. Participates in a signaling cascade initiated by activated KIT and KITLG/SCF. Isoform p46Shc and isoform p52Shc, once phosphorylated, couple activated receptor tyrosine kinases to Ras via the recruitment of the GRB2/SOS complex and are implicated in the cytoplasmic propagation of mitogenic signals. Isoform p46Shc and isoform p52Shc may thus function as initiators of the Ras signaling cascade in various non-neuronal systems. Isoform p66Shc does not mediate Ras activation, but is involved in signal transduction pathways that regulate the cellular response to oxidative stress and life span. Isoform p66Shc acts as a downstream target of the tumor suppressor p53 and is indispensable for the ability of stress-activated p53 to induce elevation of intracellular oxidants, cytochrome c release and apoptosis. The expression of isoform p66Shc has been correlated with life span (By similarity). Participates in signaling downstream of the angiopoietin receptor TEK/TIE2, and plays a role in the regulation of endothelial cell migration and sprouting angiogenesis.
See full target information SHC1 phospho Y239
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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