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AB233477

Anti-SHC3/SHCC antibody

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(2 Publications)

Rabbit Polyclonal SHC3/SHCC antibody. Suitable for WB, IHC-P and reacts with Pig, Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SHC3 aa 150-450.

View Alternative Names

NSHC, SHCC, SHC3, SHC-transforming protein 3, Neuronal Shc, Protein Rai, SHC-transforming protein C, Src homology 2 domain-containing-transforming protein C3, N-Shc, SH2 domain protein C3

8 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHC3/SHCC antibody (AB233477)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHC3/SHCC antibody (AB233477)

Formalin-fixed, paraffin-embedded human kidney tissue stained for SHC3/SHCC using ab233477 at 10 μg/ml in immunohistochemical analysis. DAB staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHC3/SHCC antibody (AB233477)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHC3/SHCC antibody (AB233477)

Formalin-fixed, paraffin-embedded human stomach cancer tissue stained for SHC3/SHCC using ab233477 at 10 μg/ml in immunohistochemical analysis. DAB staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHC3/SHCC antibody (AB233477)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHC3/SHCC antibody (AB233477)

Formalin-fixed, paraffin-embedded human cerebrum tissue stained for SHC3/SHCC using ab233477 at 10 μg/ml in immunohistochemical analysis. DAB staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHC3/SHCC antibody (AB233477)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHC3/SHCC antibody (AB233477)

Formalin-fixed, paraffin-embedded human glioma tissue stained for SHC3/SHCC using ab233477 at 10 μg/ml in immunohistochemical analysis. DAB staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHC3/SHCC antibody (AB233477)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHC3/SHCC antibody (AB233477)

Formalin-fixed, paraffin-embedded human liver tissue stained for SHC3/SHCC using ab233477 at 10 μg/ml in immunohistochemical analysis. DAB staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHC3/SHCC antibody (AB233477)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHC3/SHCC antibody (AB233477)

Formalin-fixed, paraffin-embedded human breast cancer tissue stained for SHC3/SHCC using ab233477 at 10 μg/ml in immunohistochemical analysis. DAB staining.

Western blot - Anti-SHC3/SHCC antibody (AB233477)
  • WB

Supplier Data

Western blot - Anti-SHC3/SHCC antibody (AB233477)

All lanes:

Western blot - Anti-SHC3/SHCC antibody (ab233477) at 3 µg/mL

Lane 1:

Pig brain lysate

Lane 2:

HepG2 (human liver hepatocellular carcinoma cell line) cell lysate

Secondary

All lanes:

HRP-Linked Guinea pig anti-rabbit at 1/2000 dilution

Predicted band size: 64 kDa

false

Western blot - Anti-SHC3/SHCC antibody (AB233477)
  • WB

Supplier Data

Western blot - Anti-SHC3/SHCC antibody (AB233477)

All lanes:

Western blot - Anti-SHC3/SHCC antibody (ab233477) at 2 µg/mL

All lanes:

Recombinant human SHC3/SHCC protein

Predicted band size: 64 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human, Pig

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human SHC3 aa 150-450. The exact immunogen used to generate this antibody is proprietary information.

Q92529

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5-20 µg/mL", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Pig": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Antigen-specific affinity chromatography followed by Protein A affinity chromatography.
Storage buffer
pH: 7.4 Preservative: 0.011% Proclin 300 Constituents: PBS, 55.77% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SHC3 also known as SHCC is an adapter protein with a molecular weight approximately 55 kDa. It plays a significant role in signal transduction pathways by mediating interactions between activated tyrosine kinases and downstream molecules. SHC3 contains typical SH2 and PTB domains which allow it to bind phosphotyrosine residues on activated receptors. This protein is mainly expressed in the neural tissues suggesting a role in nervous system functions.
Biological function summary

SHC3 acts as a mediator in the signaling pathways that regulate cell proliferation differentiation and survival. It forms part of multi-protein complexes involved in transducing signals from the cell surface to the nucleus. Upon activation SHC3 interacts with various signaling proteins to propagate essential cellular signals that contribute to neuronal development and function. These interactions highlight its importance in maintaining cellular communication within neural environments.

Pathways

SHC3 is intricately involved in the MAPK/ERK pathway and PI3K/AKT pathway both critical for cell survival and growth. In the MAPK/ERK pathway SHC3 links receptors like Trk to downstream effectors facilitating signal transmission. It also interacts with important proteins such as Grb2 and Ras serving as a scaffold to support signaling cascades that regulate neural cell function. Through these pathways SHC3 plays an integral part in linking external cellular cues to fine-tune intracellular responses.

SHC3 has connections to neurodegenerative disorders and cancer. Abnormal activity or expression of SHC3 may contribute to neurodegenerative diseases by disrupting normal signal transduction in neurons. Additionally in certain cancers altered SHC3 signaling can influence tumor growth and survival by interacting with proteins such as PI3K. Understanding SHC3's role in these conditions could advance therapeutic strategies targeting aberrant signaling pathways.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Signaling adapter that couples activated growth factor receptors to signaling pathway in neurons. Involved in the signal transduction pathways of neurotrophin-activated Trk receptors in cortical neurons.
See full target information SHC3
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

iScience 26:106093 PubMed36843845

2023

Prenatal low-dose methylmercury exposure causes premature neuronal differentiation and autism-like behaviors in a rodent model.

Applications

Unspecified application

Species

Unspecified reactive species

Allison Loan,Joseph Wai-Hin Leung,David P Cook,Chelsea Ko,Barbara C Vanderhyden,Jing Wang,Hing Man Chan

Cell cycle (Georgetown, Tex.) 20:2174-2194 PubMed34494934

2021

NEAT1/miR-146a-3p/TrkB/ShcB axis regulates the development and function of chondrocyte.

Applications

Unspecified application

Species

Unspecified reactive species

Fanyou Ning,Shaobo Zhu,Hui Gao,Yu Deng
View all publications
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