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Rabbit Recombinant Monoclonal SHCBP1 antibody. Suitable for IP, WB and reacts with Mouse, Rat, Human samples. Cited in 5 publications.

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Images

Western blot - Anti-SHCBP1 antibody [EPR18843] (AB184467), expandable thumbnail
  • Western blot - Anti-SHCBP1 antibody [EPR18843] (AB184467), expandable thumbnail
  • Western blot - Anti-SHCBP1 antibody [EPR18843] (AB184467), expandable thumbnail
  • Immunoprecipitation - Anti-SHCBP1 antibody [EPR18843] (AB184467), expandable thumbnail
  • Western blot - Anti-SHCBP1 antibody [EPR18843] (AB184467), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWB
Human
Expected
Tested
Mouse
Tested
Tested
Rat
Expected
Tested

Tested
Tested

Species

Mouse

Dilution info

1/40

Notes

-

Expected
Expected

Species

Rat, Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/1000

Notes

-

Species

Rat

Dilution info

1/1000

Notes

-

Species

Human

Dilution info

1/1000

Notes

-

Target data

Function

May play a role in signaling pathways governing cellular proliferation, cell growth and differentiation. May be a component of a novel signaling pathway downstream of Shc. Acts as a positive regulator of FGF signaling in neural progenitor cells.

Alternative names

Recommended products

Rabbit Recombinant Monoclonal SHCBP1 antibody. Suitable for IP, WB and reacts with Mouse, Rat, Human samples. Cited in 5 publications.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR18843

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

SHCBP1 also known as SHC SH2-Domain Binding Protein 1 is a 72 kDa protein involved in cell cycle regulation. This protein exhibits a significant role in the interaction with the SHC1 adaptor protein influencing the signaling pathways activated by receptor tyrosine kinases. SHCBP1 shows high expression in tissues with active cell division such as the testis and several cancer cell lines. Its localization within cells includes both the cytoplasm and nucleus indicating diverse functional roles.

Biological function summary

SHCBP1 aids in processes fundamental to cell proliferation and differentiation. It does not operate alone but participates in larger protein complexes that modulate cell division events. By interacting with other signaling molecules SHCBP1 becomes an important player in mitotic spindle assembly and cytokinesis. Its presence in complexes highlights its interaction with additional proteins which regulates the progression of the cell cycle.

Pathways

SHCBP1 resides in pathways linked to the regulation of mitosis and cell proliferation. This protein plays an important role in the PI3K/Akt signaling pathway which is pivotal for cell growth and survival. Additionally ties to proteins like PLK1 and Aurora B further emphasize SHCBP1's integration into cell cycle regulation networks. These interactions ensure proper cellular responses to growth signals and accurate chromosome segregation during mitosis.

Associated diseases and disorders

SHCBP1 connections to cancer and infertility are notable. In several types of cancer overexpression of SHCBP1 associates with tumor progression and poor prognosis often through its interaction with oncogenic proteins like Cyclin D1. In cases of male infertility dysregulation of SHCBP1 impacts spermatogenesis due to its essential role in cell cycle and division. Understanding these associations offers potential pathways for therapeutic interventions targeting SHCBP1-related mechanisms.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Western blot - Anti-SHCBP1 antibody [EPR18843] (ab184467), expandable thumbnail

    Western blot - Anti-SHCBP1 antibody [EPR18843] (ab184467)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure times: Lane 1, 2, 3, 4, 5 and 6: 3 minutes; Lane 7 and 8: 30 seconds.

    SHCBP1 plays important roles in embryonic development, cytokinesis, neuronal development, tumorigenesis and T cell development. It is highly expressed in thymus but absent in liver. It can be induced in serum-stimulated NIH/3T3 cells. (PMID: 10086341 and 25153088).

    All lanes: Western blot - Anti-SHCBP1 antibody [EPR18843] (ab184467) at 1/5000 dilution

    Lane 1: NIH/3T3 (Mouse embryonic fibroblast cell line) treated with 0.5% FBS for 48 hours whole cell lysate at 20 µg

    Lane 2: NIH/3T3 (Mouse embryonic fibroblast cell line) treated with 0.5% FBS for 48 hours then changed to 20% FBS for 28 hours whole cell lysate at 20 µg

    Lane 3: Mouse embryo tissue lysate at 20 µg

    Lane 4: P0 mouse brain tissue lysate at 20 µg

    Lane 5: Rat embryo tissue lysate at 20 µg

    Lane 6: P0 rat brain tissue lysate at 20 µg

    Lane 7: Mouse liver tissue lysate at 20 µg

    Lane 8: Mouse thymus tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 76 kDa

    Observed band size: 76 kDa

  • Western blot - Anti-SHCBP1 antibody [EPR18843] (ab184467), expandable thumbnail

    Western blot - Anti-SHCBP1 antibody [EPR18843] (ab184467)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure times: Lane 1: 30 seconds; Lane 2: 8 seconds.

    All lanes: Western blot - Anti-SHCBP1 antibody [EPR18843] (ab184467) at 1/1000 dilution

    Lane 1: Human fetal heart tissue lysate at 10 µg

    Lane 2: Human fetal kidney tissue lysate at 10 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 76 kDa

    Observed band size: 76 kDa

  • Western blot - Anti-SHCBP1 antibody [EPR18843] (ab184467), expandable thumbnail

    Western blot - Anti-SHCBP1 antibody [EPR18843] (ab184467)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposuretimes: Lane 1, 2, 3 and 4: 30 seconds; Lane 5: 8 seconds.

    All lanes: Western blot - Anti-SHCBP1 antibody [EPR18843] (ab184467) at 1/5000 dilution

    Lane 1: Mouse lung tissue lysate at 20 µg

    Lane 2: HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

    Lane 3: A431 (Human epidermoid carcinoma cell line) whole cell lysate at 20 µg

    Lane 4: Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 20 µg

    Lane 5: Neuro-2a (Mouse neuroblastoma cell line) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 76 kDa

    Observed band size: 76 kDa

  • Immunoprecipitation - Anti-SHCBP1 antibody [EPR18843] (ab184467), expandable thumbnail

    Immunoprecipitation - Anti-SHCBP1 antibody [EPR18843] (ab184467)

    SHCBP1 was immunoprecipitated from 0.35 mg of Mouse thymus whole cell lysate with ab184467 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab184467 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/10000 dilution.

    Lane 1: Mouse thymus whole cell lysate, 10μg (Input).

    Lane 2: ab184467 IP in Mouse thymus whole cell lysate.

    Lane 3: Rabbit IgG,monoclonal [EPR25A]-Isotype Control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab184467 in Mouse thymus whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 seconds.

    All lanes: Immunoprecipitation - Anti-SHCBP1 antibody [EPR18843] (ab184467)

    Predicted band size: 76 kDa

  • Western blot - Anti-SHCBP1 antibody [EPR18843] (ab184467), expandable thumbnail

    Western blot - Anti-SHCBP1 antibody [EPR18843] (ab184467)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-SHCBP1 antibody [EPR18843] (ab184467) at 1/1000 dilution

    Lane 1: C6 (Rat glial tumor cell line) whole cell lysate at 20 µg

    Lane 2: PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate at 20 µg

    Lane 3: RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate at 20 µg

    Lane 4: NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 76 kDa

    Observed band size: 76 kDa

    Exposure time: 10s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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