Anti-SHCBP1 antibody [EPR18843] - BSA and Azide free

• IP

Supplier Data

Immunoprecipitation - Anti-SHCBP1 antibody [EPR18843] - BSA and Azide free (AB250749)

This data was developed using ab184467, the same antibody clone in a different buffer formulation.

SHCBP1 was immunoprecipitated from 0.35 mg of Mouse thymus whole cell lysate with ab184467 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab184467 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

Lane 1 : Mouse thymus whole cell lysate, 10μg (Input).
Lane 2 : ab184467 IP in Mouse thymus whole cell lysate.
Lane 3 : Rabbit IgG,monoclonal [EPR25A]-Isotype Control (ab172730) instead of ab184467 in Mouse thymus whole cell lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 3 seconds.

All lanes:

Immunoprecipitation - Anti-SHCBP1 antibody [EPR18843] (<a href='/en-us/products/primary-antibodies/shcbp1-antibody-epr18843-ab184467'>ab184467</a>)

Predicted band size: 76 kDa

false

• WB

Supplier Data

Western blot - Anti-SHCBP1 antibody [EPR18843] - BSA and Azide free (AB250749)

This data was developed using ab184467, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure times : Lane 1 : 30 seconds; Lane 2 : 8 seconds.

All lanes:

Western blot - Anti-SHCBP1 antibody [EPR18843] (<a href='/en-us/products/primary-antibodies/shcbp1-antibody-epr18843-ab184467'>ab184467</a>) at 1/1000 dilution

Lane 1:

Human fetal heart tissue lysate at 10 µg

Lane 2:

Human fetal kidney tissue lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 76 kDa

Observed band size: 76 kDa

false

• WB

Supplier Data

Western blot - Anti-SHCBP1 antibody [EPR18843] - BSA and Azide free (AB250749)

This data was developed using ab184467, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure times : Lanes 1-4 : 30 seconds; Lane 5 : 8 seconds.

All lanes:

Western blot - Anti-SHCBP1 antibody [EPR18843] (<a href='/en-us/products/primary-antibodies/shcbp1-antibody-epr18843-ab184467'>ab184467</a>) at 1/5000 dilution

Lane 1:

Mouse lung tissue lysate at 20 µg

Lane 2:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

Lane 3:

A431 (Human epidermoid carcinoma cell line) whole cell lysate at 20 µg

Lane 4:

Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 20 µg

Lane 5:

Neuro-2a (Mouse neuroblastoma cell line) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 76 kDa

Observed band size: 76 kDa

false

• WB

Supplier Data

Western blot - Anti-SHCBP1 antibody [EPR18843] - BSA and Azide free (AB250749)

This data was developed using ab184467, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure times : Lanes 1-6 : 3 minutes; Lanes 7-8 : 30 seconds.

SHCBP1 plays important roles in embryonic development, cytokinesis, neuronal development, tumorigenesis and T cell development. It is highly expressed in thymus but absent in liver. It can be induced in serum-stimulated NIH/3T3 cells. (PMID : 10086341 and 25153088).

All lanes:

Western blot - Anti-SHCBP1 antibody [EPR18843] (<a href='/en-us/products/primary-antibodies/shcbp1-antibody-epr18843-ab184467'>ab184467</a>) at 1/5000 dilution

Lane 1:

NIH/3T3 (Mouse embryonic fibroblast cell line) treated with 0.5% FBS for 48 hours whole cell lysate at 20 µg

Lane 2:

NIH/3T3 (Mouse embryonic fibroblast cell line) treated with 0.5% FBS for 48 hours then changed to 20% FBS for 28 hours whole cell lysate at 20 µg

Lane 3:

Mouse embryo tissue lysate at 20 µg

Lane 4:

P0 mouse brain tissue lysate at 20 µg

Lane 5:

Rat embryo tissue lysate at 20 µg

Lane 6:

P0 rat brain tissue lysate at 20 µg

Lane 7:

Mouse liver tissue lysate at 20 µg

Lane 8:

Mouse thymus tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 76 kDa

Observed band size: 76 kDa

false

• WB

Supplier Data

Western blot - Anti-SHCBP1 antibody [EPR18843] - BSA and Azide free (AB250749)

This data was developed using ab184467, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-SHCBP1 antibody [EPR18843] (<a href='/en-us/products/primary-antibodies/shcbp1-antibody-epr18843-ab184467'>ab184467</a>) at 1/1000 dilution

Lane 1:

C6 (Rat glial tumor cell line) whole cell lysate at 20 µg

Lane 2:

PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate at 20 µg

Lane 3:

RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate at 20 µg

Lane 4:

NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 76 kDa

Observed band size: 76 kDa

false

Exposure time: 10s