JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB236588

Anti-SHE antibody

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Polyclonal SHE antibody. Suitable for WB, IHC-P and reacts with Rat, Mouse, Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human SHE aa 1-200.

View Alternative Names

SH2 domain-containing adapter protein E, SHE

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHE antibody (AB236588)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHE antibody (AB236588)

Paraffin-embedded human pancreas tissue stained for SHE using ab236588 at 1/100 dilution in immunohistochemical analysis.

Western blot - Anti-SHE antibody (AB236588)
  • WB

Supplier Data

Western blot - Anti-SHE antibody (AB236588)

All lanes:

Western blot - Anti-SHE antibody (ab236588) at 1/500 dilution

Lane 1:

Rat heart lysate

Lane 2:

Mouse heart lysate

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/50000 dilution

Predicted band size: 54 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human SHE aa 1-200. The exact immunogen used to generate this antibody is proprietary information.

Q5VZ18

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purity >95%.
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein SHE also known as Src homology 2 domain-containing transforming protein 1 (SHC1) has a molecular weight of approximately 66 kDa. SHE functions as an adapter molecule within cells meaning it helps in signal transduction by facilitating phosphorylation events. SHE is widely expressed in a range of tissues including the brain heart and hematopoietic cells. Its presence is critical for linking receptor activation to downstream signalling cascades binding to phosphorylated tyrosine sites on growth factor receptors and certain intracellular kinases.
Biological function summary

The role of SHE involves the propagation of signals related to cell growth and differentiation. As part of several multi-protein complexes SHE serves as an essential mediator in cellular pathways. It achieves this by recruiting other proteins to the membrane after growth factor receptor activation promoting the assembly of signalling complexes. SHE plays an important role in regulating responses to various stimuli by interacting with proteins like Grb2 and Ras influencing pathways that lead to cellular proliferation and survival.

Pathways

Several cellular mechanisms heavily rely on SHE's function. It actively participates in the MAPK/ERK pathway important for transmitting growth signals from the cell membrane to the nucleus. SHE also contributes to the PI3K/AKT pathway which governs cell survival and metabolism. In these pathways SHE frequently interacts with proteins such as EGFR (Epidermal Growth Factor Receptor) and IRS (Insulin Receptor Substrate) ensuring the specificity and accuracy of the cellular responses triggered by these pathways.

SHE's interactions significantly impact cancer and cardiovascular disease. Aberrant activation or expression of SHE has been linked to tumorigenesis where it facilitates uncontrolled cell growth by misregulating pathways like MAPK/ERK. In cardiovascular disorders changes in SHE expression can influence heart cell responses to stress leading to abnormal heart functions. Proteins like Bcl-2 and Akt known for their roles in cell survival and stress responses are connected to SHE in the context of these diseases highlighting its importance in disease pathophysiology.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

See full target information SHE
style
left-column
style
left-column
style
right-column

Complementary Products

Primary Antibodies

AB155768

Anti-TOX antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOX antibody (AB155768)

  • 4
  • 1
Primary Antibodies

AB235366

Anti-DHX35 antibody

Western blot - Anti-DHX35 antibody (AB235366)

  • 0
  • 0
Primary Antibodies

AB111703

Anti-ILT-7 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ILT-7 antibody (AB111703)

  • 0
  • 0
Primary Antibodies

AB2728

Anti-Cytohesin 2 antibody [10A12]

Immunocytochemistry/ Immunofluorescence - Anti-Cytohesin 2 antibody [10A12] (AB2728)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com