Anti-SHIP-1 antibody [EP378Y] - BSA and Azide free (ab190551)

Rabbit Monoclonal SHIP-1 antibody. Carrier free. Suitable for IHC-P, ICC/IF, IP, WB, Flow Cyt (Intra) and reacts with Mouse, Human samples.

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Images

Western blot - Anti-SHIP-1 antibody [EP378Y] - BSA and Azide free (AB190551), expandable thumbnail

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: PBS
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IHC-P	ICC/IF	IP	WB	Flow Cyt (Intra)
Human	Tested	Tested	Tested	Tested	Tested
Mouse	Tested	Expected	Expected	Expected	Expected

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info -	Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.
Species Human	Dilution info -	Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse	Dilution info Use at an assay dependent concentration.	Notes -

Associated Products

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3 products for Alternative Version

Target data

See full target information INPP5D

Function

Phosphatidylinositol (PtdIns) phosphatase that specifically hydrolyzes the 5-phosphate of phosphatidylinositol-3,4,5-trisphosphate (PtdIns(3,4,5)P3) to produce PtdIns(3,4)P2, thereby negatively regulating the PI3K (phosphoinositide 3-kinase) pathways (PubMed:10764818, PubMed:8723348, PubMed:8769125). Able also to hydrolyzes the 5-phosphate of phosphatidylinositol-4,5-bisphosphate (PtdIns(4,5)P3) and inositol 1,3,4,5-tetrakisphosphate (PubMed:10764818, PubMed:8769125, PubMed:9108392). Acts as a negative regulator of B-cell antigen receptor signaling. Mediates signaling from the FC-gamma-RIIB receptor (FCGR2B), playing a central role in terminating signal transduction from activating immune/hematopoietic cell receptor systems. Acts as a negative regulator of myeloid cell proliferation/survival and chemotaxis, mast cell degranulation, immune cells homeostasis, integrin alpha-IIb/beta-3 signaling in platelets and JNK signaling in B-cells. Regulates proliferation of osteoclast precursors, macrophage programming, phagocytosis and activation and is required for endotoxin tolerance. Involved in the control of cell-cell junctions, CD32a signaling in neutrophils and modulation of EGF-induced phospholipase C activity (PubMed:16682172). Key regulator of neutrophil migration, by governing the formation of the leading edge and polarization required for chemotaxis. Modulates FCGR3/CD16-mediated cytotoxicity in NK cells. Mediates the activin/TGF-beta-induced apoptosis through its Smad-dependent expression.

Alternative names

SHIP, SHIP1, INPP5D, Inositol polyphosphate-5-phosphatase D, Inositol polyphosphate-5-phosphatase of 145 kDa, SH2 domain-containing inositol 5'-phosphatase 1, p150Ship, SIP-145, SH2 domain-containing inositol phosphatase 1, SHIP-1, hp51CN

Recommended products

Rabbit Monoclonal SHIP-1 antibody. Carrier free. Suitable for IHC-P, ICC/IF, IP, WB, Flow Cyt (Intra) and reacts with Mouse, Human samples.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: PBS
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Carrier free: Yes
Clone number: EP378Y
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: +4°C
Storage information: Do Not Freeze

Notes

ab190551 is the carrier-free version of Anti-SHIP-1 antibody [EP378Y] ab45142.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

SHIP-1 also known as SHIP or INPP5D is a protein with a molecular mass of approximately 145 kDa. It functions as an inositol polyphosphate-5-phosphatase. This enzyme specifically hydrolyzes the 5’ phosphate from phosphatidylinositol-345-trisphosphate (PIP3) converting it to phosphatidylinositol-34-bisphosphate (PI(34)P2). SHIP-1 is mainly expressed in hematopoietic cells including those of the immune system like B cells T cells and myeloid cells.

Biological function summary

SHIP-1 plays significant roles in regulating immune cell signaling. It partakes in control over cell growth apoptosis and cellular responses to external signals. SHIP-1 influences the balance between cell survival and programmed cell death by modulating the PI3K signaling pathway which is pivotal for lymphocyte activation and immune functions. SHIP-1 can operate independently or as part of larger signaling complexes with other molecules to ensure immune cells respond appropriately to stimuli.

Pathways

SHIP-1 plays an integral role in the phosphoinositide signaling pathway and is linked to the PI3K-Akt pathway. It acts as an important regulatory node by limiting the accumulation of PIP3 a necessary component for Akt activation. This regulation allows for controlled signal propagation that influences immune cell activity and development. SHIP-1 also interfaces with proteins like PTEN and SHP-1 which similarly modulate downstream signaling events within these pathways ensuring the maintenance of immune equilibrium.

Associated diseases and disorders

SHIP-1 is associated with conditions like leukemia and autoimmune diseases. Alterations or deficiencies in SHIP-1 function can lead to abnormal immune responses or uncontrolled cell proliferation. For instance its reduced activity has connections to leukemia where there is excessive cell growth due to unchecked PI3K signaling. Moreover SHIP-1 is associated with autoimmune disorders through interactions with other signaling proteins that might affect the intensity and duration of immune responses highlighting its role in maintaining immune tolerance and preventing self-reactivity.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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12 product images

Western blot - Anti-SHIP-1 antibody [EP378Y] - BSA and Azide free (ab190551)
This data was developed using Anti-SHIP-1 antibody [EP378Y] ab45142, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-SHIP-1 antibody [EP378Y] (Anti-SHIP-1 antibody [EP378Y] ab45142) at 1/5000 dilution
Lane 1: Daudi cell lysate at 20 µg
Lane 2: Ramos cell lysate at 20 µg
Secondary
All lanes: Peroxidase-conjugated goat anti-rabbit IgG, (H+L) at 1/1000 dilution
Predicted band size: 133 kDa
Observed band size: 140 kDa
Western blot - Anti-SHIP-1 antibody [EP378Y] - BSA and Azide free (ab190551)
This data was developed using Anti-SHIP-1 antibody [EP378Y] ab45142, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-SHIP-1 antibody [EP378Y] (Anti-SHIP-1 antibody [EP378Y] ab45142) at 1/1000 dilution
All lanes: KM3 cell lysate at 20 µg
Secondary
All lanes: Peroxidase-conjugated goat anti-rabbit IgG, (H+L) at 1/1000 dilution
Predicted band size: 133 kDa
Observed band size: 140 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHIP-1 antibody [EP378Y] - BSA and Azide free (ab190551)
This data was developed using Anti-SHIP-1 antibody [EP378Y] ab45142, the same antibody clone in a different buffer formulation.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse spleen tissue labelling SHIP-1 with purified Anti-SHIP-1 antibody [EP378Y] ab45142 at 1/500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. Goat Anti-Rabbit IgG H&L (HRP) ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHIP-1 antibody [EP378Y] - BSA and Azide free (ab190551)
This data was developed using Anti-SHIP-1 antibody [EP378Y] ab45142, the same antibody clone in a different buffer formulation.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human spleen tissue labelling SHIP-1 with purified Anti-SHIP-1 antibody [EP378Y] ab45142 at 1/500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. Goat Anti-Rabbit IgG H&L (HRP) ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
Immunoprecipitation - Anti-SHIP-1 antibody [EP378Y] - BSA and Azide free (ab190551)
This data was developed using Anti-SHIP-1 antibody [EP378Y] ab45142, the same antibody clone in a different buffer formulation.
Anti-SHIP-1 antibody [EP378Y] ab45142 (purified) at 1/20 immunoprecipitating SHIP-1 in Daudi whole cell lysate.
Lane 1 (input): Daudi whole cell lysate (10μg)
Lane 2 (+): Anti-SHIP-1 antibody [EP378Y] ab45142 + Daudi whole cell lysate (10μg).
Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-SHIP-1 antibody [EP378Y] ab45142 in Daudi whole cell lysate.
For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).
Blocking and dilution buffer: 5% NFDM/TBST.
All lanes: Immunoprecipitation - Anti-SHIP-1 antibody [EP378Y] (Anti-SHIP-1 antibody [EP378Y] ab45142)
Predicted band size: 133 kDa
Observed band size: 140 kDa
Immunocytochemistry/ Immunofluorescence - Anti-SHIP-1 antibody [EP378Y] - BSA and Azide free (ab190551)
This data was developed using Anti-SHIP-1 antibody [EP378Y] ab45142, the same antibody clone in a different buffer formulation.Immunocytochemistry/Immunofluorescence analysis of Raji cells labelling SHIP-1 with purified Anti-SHIP-1 antibody [EP378Y] ab45142 at 1/150. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077, an Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291, a mouse anti-tubulin (1/1000) and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120, an Alexa Fluor^® 594-conjugated goat anti-mouse IgG (1/500) were also used. Control 1: primary antibody (1/150) and secondary antibody, Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120, an Alexa Fluor^® 594-conjugated goat anti-mouse IgG (1/500). Control 2: Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (1/1000) and secondary antibody, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077, an Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/500).
Immunocytochemistry/ Immunofluorescence - Anti-SHIP-1 antibody [EP378Y] - BSA and Azide free (ab190551)
This data was developed using Anti-SHIP-1 antibody [EP378Y] ab45142, the same antibody clone in a different buffer formulation.Immunocytochemistry/Immunofluorescence analysis of Jurkat cells labelling SHIP-1 with purified Anti-SHIP-1 antibody [EP378Y] ab45142 at 1/150. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077, an Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291, a mouse anti-tubulin (1/1000) and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120, an Alexa Fluor^® 594-conjugated goat anti-mouse IgG (1/500) were also used. Control 1: primary antibody (1/150) and secondary antibody, Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120, an Alexa Fluor^® 594-conjugated goat anti-mouse IgG (1/500). Control 2: Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (1/1000) and secondary antibody, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077, an Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/500).
Flow Cytometry (Intracellular) - Anti-SHIP-1 antibody [EP378Y] - BSA and Azide free (ab190551)
This data was developed using Anti-SHIP-1 antibody [EP378Y] ab45142, the same antibody clone in a different buffer formulation.
Intracellular Flow Cytometry analysis of Raji cells labelling SHIP-1 with purified Anti-SHIP-1 antibody [EP378Y] ab45142 at 1/50 (red). Cells were fixed with 2% paraformaldehyde. A FITC-conjugated goat anti-rabbit IgG (1/150) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.
Western blot - Anti-SHIP-1 antibody [EP378Y] - BSA and Azide free (ab190551)
This data was developed using Anti-SHIP-1 antibody [EP378Y] ab45142, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-SHIP-1 antibody [EP378Y] (Anti-SHIP-1 antibody [EP378Y] ab45142) at 1/50000 dilution
All lanes: Daudi cell lysate at 10 µg
Secondary
All lanes: HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 133 kDa
Observed band size: 140 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHIP-1 antibody [EP378Y] - BSA and Azide free (ab190551)
This data was developed using Anti-SHIP-1 antibody [EP378Y] ab45142, the same antibody clone in a different buffer formulation.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human spleen tissue labelling SHIP-1 with unpurified Anti-SHIP-1 antibody [EP378Y] ab45142 at a dilution of 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Flow Cytometry (Intracellular) - Anti-SHIP-1 antibody [EP378Y] - BSA and Azide free (ab190551)
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-SHIP-1 antibody [EP378Y] ab45142).
Flow cytometry overlay histogram showing left Raji positive cells and right negative MCF7 stained with Anti-SHIP-1 antibody [EP378Y] ab45142 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilised with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS containing 10μg/ml human IgG and 10% normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (Anti-SHIP-1 antibody [EP378Y] ab45142) (1x 106 in 100μl at 0.008μg/ml (1/275000)) for 30min at 22°C.

The secondary antibody Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed was incubated at 1/4000 for 30min at 22°C

Isotype control antibody (black line) was Recombinant Rabbit IgG, monoclonal [EPR25A] - Isotype Control used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

Acquisition of >5000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter.
Western blot - Anti-SHIP-1 antibody [EP378Y] - BSA and Azide free (ab190551)
This data was developed using Anti-SHIP-1 antibody [EP378Y] ab45142, the same antibody clone in a different buffer formulation.
Anti-SHIP-1 antibody [EP378Y] ab45142 was shown to react with INPP5D in wild-type iPSC-derived microglia cells in Western blot with loss of signal observed in a INPP5D knockout cell line. Wild-type iPSC-derived microglia and INPP5D knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 5% milk in TBST for 1 hr before incubation with Anti-SHIP-1 antibody [EP378Y] ab45142 overnight at 4 °C at a 1/500 dilution. Blots were incubated with secondary antibodies at 0.2 µg/mL before imaging.

These data were provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins. Abcam and YCharOS are working together to help address the reproducibility crisis by enabling the life science community to better evaluate commercially available antibodies.
All lanes: Western blot - Anti-SHIP-1 antibody [EP378Y] (Anti-SHIP-1 antibody [EP378Y] ab45142) at 1/500 dilution
Lane 1: Wild-type iPSC-derived microglia lysate at 10 µg
Lane 2: INPP5D knock-out iPSC-derived microglia lysate at 10 µg