JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB190552

Anti-SHIP-1 (phospho Y1020) antibody [EPR425] - BSA and Azide free

Be the first to review this product! Submit a review

|

(1 Publication)

Rabbit Recombinant Monoclonal SHIP-1 phospho Y1020 antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

SHIP, SHIP1, INPP5D, Inositol polyphosphate-5-phosphatase D, Inositol polyphosphate-5-phosphatase of 145 kDa, SH2 domain-containing inositol 5'-phosphatase 1, p150Ship, SIP-145, SH2 domain-containing inositol phosphatase 1, SHIP-1, hp51CN

1 Images
Western blot - Anti-SHIP-1 (phospho Y1020) antibody [EPR425] - BSA and Azide free (AB190552)
  • WB

Lab

Western blot - Anti-SHIP-1 (phospho Y1020) antibody [EPR425] - BSA and Azide free (AB190552)

This data was developed using ab96402, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-SHIP-1 (phospho Y1020) antibody [EPR425] (<a href='/en-us/products/primary-antibodies/ship-1-phospho-y1020-antibody-epr425-ab96402'>ab96402</a>) at 1/2000 dilution

Lane 1:

Raji whole cell lysate - untreated at 10 µg

Lane 2:

Raji whole cell lysate - treated with pervanadate at 10 µg

Secondary

All lanes:

Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 133 kDa

Observed band size: 145 kDa

false

Exposure time: 3min

  • Unconjugated

    Anti-SHIP-1 (phospho Y1020) antibody [EPR425]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR425

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Complementary Products

Assay Kits

AB279921

Phospho-SHIP-1 (Y1020) ELISA Kit

Sandwich ELISA - Phospho-SHIP-1 (Y1020) ELISA Kit (AB279921)

  • 0
  • 0
Primary Antibodies

AB45142

Anti-SHIP-1 antibody [EP378Y]

20ul selling size|KO Validated|RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-SHIP-1 antibody [EP378Y] (AB45142)

  • 0
  • 0
Primary Antibodies

AB32559

Anti-SHP1 antibody [Y476]

BOND RX™ Validated|RabMAb|Recombinant|KO Validated|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHP1 antibody [Y476] (AB32559)

  • 5
  • 3
Primary Antibodies

AB68423

Anti-CD32B (phospho Y292) antibody [EP926Y]

20ul selling size|RabMAb|Recombinant|Trial Size

Immunoprecipitation - Anti-CD32B (phospho Y292) antibody [EP926Y] (AB68423)

  • 0
  • 0
Primary Antibodies

AB45143

Anti-CD32B antibody [EP888Y]

20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD32B antibody [EP888Y] (AB45143)

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
style
left-column

Product details

ab190552 is the carrier-free version of ab96402.

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SHIP-1 also known as SHIP or INPP5D is a protein with a molecular mass of approximately 145 kDa. It functions as an inositol polyphosphate-5-phosphatase. This enzyme specifically hydrolyzes the 5’ phosphate from phosphatidylinositol-345-trisphosphate (PIP3) converting it to phosphatidylinositol-34-bisphosphate (PI(34)P2). SHIP-1 is mainly expressed in hematopoietic cells including those of the immune system like B cells T cells and myeloid cells.
Biological function summary

SHIP-1 plays significant roles in regulating immune cell signaling. It partakes in control over cell growth apoptosis and cellular responses to external signals. SHIP-1 influences the balance between cell survival and programmed cell death by modulating the PI3K signaling pathway which is pivotal for lymphocyte activation and immune functions. SHIP-1 can operate independently or as part of larger signaling complexes with other molecules to ensure immune cells respond appropriately to stimuli.

Pathways

SHIP-1 plays an integral role in the phosphoinositide signaling pathway and is linked to the PI3K-Akt pathway. It acts as an important regulatory node by limiting the accumulation of PIP3 a necessary component for Akt activation. This regulation allows for controlled signal propagation that influences immune cell activity and development. SHIP-1 also interfaces with proteins like PTEN and SHP-1 which similarly modulate downstream signaling events within these pathways ensuring the maintenance of immune equilibrium.

SHIP-1 is associated with conditions like leukemia and autoimmune diseases. Alterations or deficiencies in SHIP-1 function can lead to abnormal immune responses or uncontrolled cell proliferation. For instance its reduced activity has connections to leukemia where there is excessive cell growth due to unchecked PI3K signaling. Moreover SHIP-1 is associated with autoimmune disorders through interactions with other signaling proteins that might affect the intensity and duration of immune responses highlighting its role in maintaining immune tolerance and preventing self-reactivity.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Phosphatidylinositol (PtdIns) phosphatase that specifically hydrolyzes the 5-phosphate of phosphatidylinositol-3,4,5-trisphosphate (PtdIns(3,4,5)P3) to produce PtdIns(3,4)P2, thereby negatively regulating the PI3K (phosphoinositide 3-kinase) pathways (PubMed : 10764818, PubMed : 8723348, PubMed : 8769125). Able also to hydrolyzes the 5-phosphate of phosphatidylinositol-4,5-bisphosphate (PtdIns(4,5)P3) and inositol 1,3,4,5-tetrakisphosphate (PubMed : 10764818, PubMed : 8769125, PubMed : 9108392). Acts as a negative regulator of B-cell antigen receptor signaling. Mediates signaling from the FC-gamma-RIIB receptor (FCGR2B), playing a central role in terminating signal transduction from activating immune/hematopoietic cell receptor systems. Acts as a negative regulator of myeloid cell proliferation/survival and chemotaxis, mast cell degranulation, immune cells homeostasis, integrin alpha-IIb/beta-3 signaling in platelets and JNK signaling in B-cells. Regulates proliferation of osteoclast precursors, macrophage programming, phagocytosis and activation and is required for endotoxin tolerance. Involved in the control of cell-cell junctions, CD32a signaling in neutrophils and modulation of EGF-induced phospholipase C activity (PubMed : 16682172). Key regulator of neutrophil migration, by governing the formation of the leading edge and polarization required for chemotaxis. Modulates FCGR3/CD16-mediated cytotoxicity in NK cells. Mediates the activin/TGF-beta-induced apoptosis through its Smad-dependent expression.
See full target information INPP5D pY1020
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 14:603 PubMed36746939

2023

LSD1/PRMT6-targeting gene therapy to attenuate androgen receptor toxic gain-of-function ameliorates spinobulbar muscular atrophy phenotypes in flies and mice.

Applications

Unspecified application

Species

Unspecified reactive species

Ramachandran Prakasam,Angela Bonadiman,Roberta Andreotti,Emanuela Zuccaro,Davide Dalfovo,Caterina Marchioretti,Debasmita Tripathy,Gianluca Petris,Eric N Anderson,Alice Migazzi,Laura Tosatto,Anna Cereseto,Elena Battaglioli,Gianni Sorarù,Wooi Fang Lim,Carlo Rinaldi,Fabio Sambataro,Naemeh Pourshafie,Christopher Grunseich,Alessandro Romanel,Udai Bhan Pandey,Andrea Contestabile,Giuseppe Ronzitti,Manuela Basso,Maria Pennuto
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com