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AB243482

Anti-SHLD1 antibody

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Rabbit Polyclonal SHLD1 antibody. Suitable for ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human SHLD1 aa 50-150.

View Alternative Names

C20orf196, RINN3, SHLD1, Shieldin complex subunit 1, RINN1-REV7-interacting novel NHEJ regulator 3, Shield complex subunit 1

1 Images
Immunocytochemistry/ Immunofluorescence - Anti-SHLD1 antibody (AB243482)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SHLD1 antibody (AB243482)

PFA fixed, Triton X-100 permeabilized U-251 MG (Human brain glioma cell line) cells labeling SHLD1 using ab243482 at 4 μg/ml (green) in ICC/IF. Nuclear probe is visualized in blue, microtublues in red.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human SHLD1 aa 50-150. The exact immunogen used to generate this antibody is proprietary information.

Q8IYI0

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SHLD1 also known as shieldin complex subunit 1 functions as part of the shieldin complex involved in DNA repair. SHLD1 has a molecular mass of approximately 22 kDa. It is expressed in various tissues and is most abundant in cells that are actively dividing such as those found in cancer cell lines. SHLD1 plays a mechanical role by acting as a scaffold protein that contributes to the non-homologous end joining (NHEJ) pathway of DNA double-strand break repair.
Biological function summary

SHLD1 participates as a component of the shieldin complex alongside SHLD2 SHLD3 and REV7. Together these proteins facilitate the repair of DNA double-strand breaks by directing 53BP1-mediated NHEJ. This complex stabilizes the DNA ends and assists in their processing which prevents excessive resection and promotes the proper end-joining process. SHLD1's role contributes significantly to maintaining genomic integrity.

Pathways

SHLD1 integrates within the DNA damage response specifically the NHEJ pathway. This pathway is important for fixing double-strand breaks and involves several other proteins like KU70 KU80 DNA-PKcs and 53BP1. SHLD1 supports the action of 53BP1 ensuring its recruitment to DNA damage sites which helps initiate and regulate the repair process.

SHLD1's involvement in genomic stability links it to cancer and immunodeficiency syndromes. Aberrations in the NHEJ pathway influenced by SHLD1 dysfunction can lead to tumorigenesis as improper DNA repair may trigger mutations and chromosomal rearrangements. SHLD1 also relates to proteins like BRCA1 which is known for its role in homologous recombination repair pathways. Understanding SHLD1 function has potential implications in developing treatments that enhance DNA repair capacity in disease contexts.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the shieldin complex, which plays an important role in repair of DNA double-stranded breaks (DSBs). During G1 and S phase of the cell cycle, the complex functions downstream of TP53BP1 to promote non-homologous end joining (NHEJ) and suppress DNA end resection. Mediates various NHEJ-dependent processes including immunoglobulin class-switch recombination, and fusion of unprotected telomeres.
See full target information SHLD1
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