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AB155230

Anti-SHMT2/SHMT antibody

4

(1 Review)

|

(2 Publications)

Rabbit Polyclonal SHMT2/SHMT antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human Serine hydroxymethyltransferase, mitochondrial aa 1-500.

View Alternative Names

SHMT, Glycine hydroxymethyltransferase, Serine methylase, SHMT2

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHMT2/SHMT antibody (AB155230)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHMT2/SHMT antibody (AB155230)

Immunohistochemical analysis of paraffin embedded HeLa xenograft labeling SHMT2/SHMT with ab155230 antibody at 1/500.

Immunocytochemistry/ Immunofluorescence - Anti-SHMT2/SHMT antibody (AB155230)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SHMT2/SHMT antibody (AB155230)

Immunofluorescence analysis of HeLa cells fixed in ice-cold MeOH for 1 sec labelling SHMT2 protein at Mitochondria using ab155230 at a 1/500 dilution (Green). Hoechst 33342 was used for nuclear staining (Blue).

Western blot - Anti-SHMT2/SHMT antibody (AB155230)
  • WB

Supplier Data

Western blot - Anti-SHMT2/SHMT antibody (AB155230)

Samples were separated by 7.5% SDS-PAGE.

All lanes:

Western blot - Anti-SHMT2/SHMT antibody (ab155230) at 1/5000 dilution

Lane 1:

A431 whole cell lysates at 30 µg

Lane 2:

HepG2 whole cell lysates at 30 µg

Predicted band size: 56 kDa

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Western blot - Anti-SHMT2/SHMT antibody (AB155230)
  • WB

Unknown

Western blot - Anti-SHMT2/SHMT antibody (AB155230)

7.5% SDS PAGE

All lanes:

Western blot - Anti-SHMT2/SHMT antibody (ab155230) at 1/10000 dilution

Lane 1:

293T whole cell lysate at 10 µg

Lane 2:

HeLa whole cell lysate at 10 µg

Predicted band size: 56 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human Serine hydroxymethyltransferase, mitochondrial aa 1-500. The exact immunogen used to generate this antibody is proprietary information.

P34897

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000 - 1/20000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 20% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Serine hydroxymethyltransferase 2 (SHMT2) also known as SHMT is an enzyme that plays a role in the mitochondrial compartment. It has a molecular mass of approximately 53 kDa. SHMT2 is highly expressed in tissues with elevated rates of oxidative metabolism such as the liver and brain. The enzyme catalyzes the reversible conversion of serine to glycine with the concomitant production of 510-methylenetetrahydrofolate a critical molecule for DNA synthesis and repair.
Biological function summary

The enzyme facilitates essential reactions in mitochondrial nucleotide synthesis. SHMT2 operates within a tetrameric complex that provides one-carbon units for cellular biosynthetic processes. The activity of SHMT2 links mitochondrial serine utilization directly to the folate cycle which is essential for the synthesis of purines and thymidylate. The efficient production of these nucleotides is important for rapidly dividing cells supporting their proliferation and maintenance.

Pathways

SHMT2 takes part in the folate and methionine metabolism pathways. It interacts with proteins like methylenetetrahydrofolate dehydrogenase and thymidylate synthase which are essential for one-carbon metabolism. These interactions integrate SHMT2 activity into a larger network that balances the supply of nucleotides according to the cellular demand impacting vital pathways like DNA replication and repair.

Elevated SHMT2 expression has been linked to cancer where it supports the rapid proliferation of tumor cells by providing necessary nucleotides. Its role in the mitochondrial folate pathway is also significant in neurodegenerative disorders. SHMT2 in conjunction with other proteins such as methionine synthase may play a role in the pathology of diseases like Alzheimer's by influencing homocysteine levels and oxidative stress in brain tissues.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the cleavage of serine to glycine accompanied with the production of 5,10-methylenetetrahydrofolate, an essential intermediate for purine biosynthesis (PubMed : 24075985, PubMed : 25619277, PubMed : 29364879, PubMed : 33015733). Serine provides the major source of folate one-carbon in cells by catalyzing the transfer of one carbon from serine to tetrahydrofolate (PubMed : 25619277). Contributes to the de novo mitochondrial thymidylate biosynthesis pathway via its role in glycine and tetrahydrofolate metabolism : thymidylate biosynthesis is required to prevent uracil accumulation in mtDNA (PubMed : 21876188). Also required for mitochondrial translation by producing 5,10-methylenetetrahydrofolate; 5,10-methylenetetrahydrofolate providing methyl donors to produce the taurinomethyluridine base at the wobble position of some mitochondrial tRNAs (PubMed : 29364879, PubMed : 29452640). Associates with mitochondrial DNA (PubMed : 18063578). In addition to its role in mitochondria, also plays a role in the deubiquitination of target proteins as component of the BRISC complex : required for IFNAR1 deubiquitination by the BRISC complex (PubMed : 24075985).
See full target information Serine hydroxymethyltransferase, mitochondrial
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Bioengineered 13:8785-8797 PubMed35333683

2022

Serine hydroxymethyltransferase 2 (SHMT2) potentiates the aggressive process of oral squamous cell carcinoma by binding to interleukin enhancer-binding factor 2 (ILF2).

Applications

Unspecified application

Species

Unspecified reactive species

Hui Zhang,Yilei Che,Bin Xuan,Xiaozhen Wu,Hui Li

Molecular oncology 16:630-647 PubMed34520622

2021

The epithelial splicing regulator ESRP2 is epigenetically repressed by DNA hypermethylation in Wilms tumour and acts as a tumour suppressor.

Applications

Unspecified application

Species

Unspecified reactive species

Danny Legge,Ling Li,Whei Moriarty,David Lee,Marianna Szemes,Asef Zahed,Leonidas Panousopoulos,Wan Yun Chung,Yara Aghabi,Jasmin Barratt,Richard Williams,Kathy Pritchard-Jones,Karim T A Malik,Sebastian Oltean,Keith W Brown
View all publications
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