AB224428

Anti-SHMT2/SHMT antibody

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(4 Publications)

Rabbit Polyclonal SHMT2/SHMT antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human Serine hydroxymethyltransferase, mitochondrial aa 400-500.

View Alternative Names

SHMT, Glycine hydroxymethyltransferase, Serine methylase, SHMT2

12 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHMT2/SHMT antibody (AB224428)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHMT2/SHMT antibody (AB224428)

Paraffin-embedded human skeletal muscle tissue stained for SHMT2/SHMT using ab224428 at 1/200 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHMT2/SHMT antibody (AB224428)

Paraffin-embedded human liver tissue stained for SHMT2/SHMT using ab224428 at 1/200 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHMT2/SHMT antibody (AB224428)

Paraffin-embedded human lymph node tissue stained for SHMT2/SHMT using ab224428 at 1/200 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHMT2/SHMT antibody (AB224428)

Paraffin-embedded human colon tissue stained for SHMT2/SHMT using ab224428 at 1/200 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHMT2/SHMT antibody (AB224428)

Paraffin-embedded human stomach tissue stained for SHMT2/SHMT using ab224428 at 1/200 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-SHMT2/SHMT antibody (AB224428)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SHMT2/SHMT antibody (AB224428)

PFA-fixed, Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells stained for SHMT2/SHMT (green) using ab224428 at 4 μg/ml in ICC/IF.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHMT2/SHMT antibody (AB224428)

Paraffin-embedded human skin tissue stained for SHMT2/SHMT using ab224428 at 1/200 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHMT2/SHMT antibody (AB224428)

Paraffin-embedded human kidney tissue stained for SHMT2/SHMT using ab224428 at 1/200 dilution in immunohistochemical analysis.

Supplier Data

Western blot - Anti-SHMT2/SHMT antibody (AB224428)

All lanes:

Western blot - Anti-SHMT2/SHMT antibody (ab224428) at 1/100 dilution

Lane 1:

RT-4 cell lysate

Lane 2:

U-251 MG cell lysate

Predicted band size: 56 kDa

false

Supplier Data

Western blot - Anti-SHMT2/SHMT antibody (AB224428)

All lanes:

Western blot - Anti-SHMT2/SHMT antibody (ab224428) at 1/100 dilution

Lane 1:

NIH/3T3 (mouse embryonic fibroblast cell line) cell lysate

Lane 2:

NBT-II cell lysate

Predicted band size: 56 kDa

true

Lab

Western blot - Anti-SHMT2/SHMT antibody (AB224428)

Western blot : Rabbit Polyclonal to SHMT2/SHMT ab224428 staining at 1/100 dilution, shown in green; Mouse anti GAPDH (ab8245) loading control staining at 1/20,000 dilution, shown in magenta. A band was observed at 55 kDa in Wild-type A549 cell lysates with no signal observed at this size in SHMT2 knockout A549 cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3pc Milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-SHMT2/SHMT antibody (ab224428) at 1/100 dilution

Lane 1:

Wild-type A549 at 20 µg

Lane 2:

Western blot - Human SHMT2 knockout A549 cell line (<a href='/en-us/products/cell-lines/human-shmt2-knockout-a549-cell-line-ab301223'>ab301223</a>) at 20 µg

Lane 3:

HeLa at 20 µg

Secondary

Lanes 1 - 3:

Goat anti-Rabbit 800CW at 1/20000 dilution

Lanes 1 - 3:

Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 56 kDa

Observed band size: 55 kDa,37 kDa

false

Supplier Data

Western blot - Anti-SHMT2/SHMT antibody (AB224428)

All lanes:

Western blot - Anti-SHMT2/SHMT antibody (ab224428) at 100 µg

All lanes:

RH-30 cell lysate

Predicted band size: 56 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human Serine hydroxymethyltransferase, mitochondrial aa 400-500. The exact immunogen used to generate this antibody is proprietary information.

P34897

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/100", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "Fixation/Permeabilization: PFA/Triton X-100." }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rabbit": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Serine hydroxymethyltransferase 2 (SHMT2) also known as SHMT is an enzyme that plays a role in the mitochondrial compartment. It has a molecular mass of approximately 53 kDa. SHMT2 is highly expressed in tissues with elevated rates of oxidative metabolism such as the liver and brain. The enzyme catalyzes the reversible conversion of serine to glycine with the concomitant production of 510-methylenetetrahydrofolate a critical molecule for DNA synthesis and repair.

Biological function summary

The enzyme facilitates essential reactions in mitochondrial nucleotide synthesis. SHMT2 operates within a tetrameric complex that provides one-carbon units for cellular biosynthetic processes. The activity of SHMT2 links mitochondrial serine utilization directly to the folate cycle which is essential for the synthesis of purines and thymidylate. The efficient production of these nucleotides is important for rapidly dividing cells supporting their proliferation and maintenance.

Pathways

SHMT2 takes part in the folate and methionine metabolism pathways. It interacts with proteins like methylenetetrahydrofolate dehydrogenase and thymidylate synthase which are essential for one-carbon metabolism. These interactions integrate SHMT2 activity into a larger network that balances the supply of nucleotides according to the cellular demand impacting vital pathways like DNA replication and repair.

Elevated SHMT2 expression has been linked to cancer where it supports the rapid proliferation of tumor cells by providing necessary nucleotides. Its role in the mitochondrial folate pathway is also significant in neurodegenerative disorders. SHMT2 in conjunction with other proteins such as methionine synthase may play a role in the pathology of diseases like Alzheimer's by influencing homocysteine levels and oxidative stress in brain tissues.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the cleavage of serine to glycine accompanied with the production of 5,10-methylenetetrahydrofolate, an essential intermediate for purine biosynthesis (PubMed : 24075985, PubMed : 25619277, PubMed : 29364879, PubMed : 33015733). Serine provides the major source of folate one-carbon in cells by catalyzing the transfer of one carbon from serine to tetrahydrofolate (PubMed : 25619277). Contributes to the de novo mitochondrial thymidylate biosynthesis pathway via its role in glycine and tetrahydrofolate metabolism : thymidylate biosynthesis is required to prevent uracil accumulation in mtDNA (PubMed : 21876188). Also required for mitochondrial translation by producing 5,10-methylenetetrahydrofolate; 5,10-methylenetetrahydrofolate providing methyl donors to produce the taurinomethyluridine base at the wobble position of some mitochondrial tRNAs (PubMed : 29364879, PubMed : 29452640). Associates with mitochondrial DNA (PubMed : 18063578). In addition to its role in mitochondria, also plays a role in the deubiquitination of target proteins as component of the BRISC complex : required for IFNAR1 deubiquitination by the BRISC complex (PubMed : 24075985).

See full target information Serine hydroxymethyltransferase, mitochondrial

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Cancer & metabolism 12:35 PubMed39563470

2024

Glutaminolysis is associated with mitochondrial pathway activation and can be therapeutically targeted in glioblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Kenji Miki,Mikako Yagi,Ryusuke Hatae,Ryosuke Otsuji,Takahiro Miyazaki,Katsuhiro Goto,Daiki Setoyama,Yutaka Fujioka,Yuhei Sangatsuda,Daisuke Kuga,Nayuta Higa,Tomoko Takajo,Yonezawa Hajime,Toshiaki Akahane,Akihide Tanimoto,Ryosuke Hanaya,Yuya Kunisaki,Takeshi Uchiumi,Koji Yoshimoto

Scientific reports 14:8324 PubMed38594513

2024

A study on the significance of serine hydroxymethyl transferase expression and its role in bladder cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Si-Wei Su,Xian Chen,Guang Wang,Pei Li,Tong-Xin Yang,Ke-Wei Fang,Jing Wu,Jiong-Ming Li

Molecular cell 80:525-540.e9 PubMed33068521

2020

Characterization of Metabolic Patterns in Mouse Oocytes during Meiotic Maturation.

Applications

Unspecified application

Species

Unspecified reactive species

Ling Li,Shuai Zhu,Wenjie Shu,Yueshuai Guo,Yusheng Guan,Juan Zeng,Haichao Wang,Longsen Han,Jiaqi Zhang,Xiaohui Liu,Chunling Li,Xiaojing Hou,Min Gao,Juan Ge,Chao Ren,Hao Zhang,Tim Schedl,Xuejiang Guo,Minjian Chen,Qiang Wang

Bioscience reports 40: PubMed31894856

2020

ERRα activates SHMT2 transcription to enhance the resistance of breast cancer to lapatinib via modulating the mitochondrial metabolic adaption.

Applications

Unspecified application

Species

Unspecified reactive species

Xin Li,Kejing Zhang,Yu Hu,Na Luo

View all publications

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Product promise

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Anti-SHMT2/SHMT antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHMT2/SHMT antibody (AB224428)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHMT2/SHMT antibody (AB224428)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHMT2/SHMT antibody (AB224428)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHMT2/SHMT antibody (AB224428)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHMT2/SHMT antibody (AB224428)

Immunocytochemistry/ Immunofluorescence - Anti-SHMT2/SHMT antibody (AB224428)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHMT2/SHMT antibody (AB224428)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHMT2/SHMT antibody (AB224428)

Western blot - Anti-SHMT2/SHMT antibody (AB224428)

Western blot - Anti-SHMT2/SHMT antibody (AB224428)

Western blot - Anti-SHMT2/SHMT antibody (AB224428)

Western blot - Anti-SHMT2/SHMT antibody (AB224428)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Product promise