Rabbit Polyclonal SHMT2/SHMT antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human Serine hydroxymethyltransferase, mitochondrial aa 400-500.
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Publications
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- Molecular cell 80:525-540.e92020Characterization of Metabolic Patterns in Mouse Oocytes during Meiotic Maturation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLing Li,Shuai Zhu,Wenjie Shu,Yueshuai Guo,Yusheng Guan,Juan Zeng,Haichao Wang,Longsen Han,Jiaqi Zhang,Xiaohui Liu,Chunling Li,Xiaojing Hou,Min Gao,Juan Ge,Chao Ren,Hao Zhang,Tim Schedl,Xuejiang Guo,Minjian Chen,Qiang WangPubMed 33068521
- Bioscience reports 40:2020ERRα activates SHMT2 transcription to enhance the resistance of breast cancer to lapatinib via modulating the mitochondrial metabolic adaption.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXin Li,Kejing Zhang,Yu Hu,Na LuoPubMed 31894856
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human Serine hydroxymethyltransferase, mitochondrial aa 400-500. The exact immunogen used to generate this antibody is proprietary information. Database link P34897
Reactivity data
Select an application| IHC-P | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Expected | Tested | Expected |
| Rat | Expected | Tested | Expected |
| Cow | Predicted | Predicted | Predicted |
| Rabbit | Predicted | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/200.00000 - 1/500.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rabbit, Cow | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 0.04000-0.40000 µg/mL | Notes - |
Species Rat | Dilution info 0.04000-0.40000 µg/mL | Notes - |
Species Human | Dilution info 0.04000-0.40000 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rabbit, Cow | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.25000-2.00000 µg/mL | Notes Fixation/Permeabilization: PFA/Triton X-100. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rabbit, Cow | Dilution info - | Notes - |
Associated Products
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6 products for Alternative Product
Target data
Function
Catalyzes the cleavage of serine to glycine accompanied with the production of 5,10-methylenetetrahydrofolate, an essential intermediate for purine biosynthesis (PubMed:24075985, PubMed:25619277, PubMed:29364879, PubMed:33015733). Serine provides the major source of folate one-carbon in cells by catalyzing the transfer of one carbon from serine to tetrahydrofolate (PubMed:25619277). Contributes to the de novo mitochondrial thymidylate biosynthesis pathway via its role in glycine and tetrahydrofolate metabolism: thymidylate biosynthesis is required to prevent uracil accumulation in mtDNA (PubMed:21876188). Also required for mitochondrial translation by producing 5,10-methylenetetrahydrofolate; 5,10-methylenetetrahydrofolate providing methyl donors to produce the taurinomethyluridine base at the wobble position of some mitochondrial tRNAs (PubMed:29364879, PubMed:29452640). Associates with mitochondrial DNA (PubMed:18063578). In addition to its role in mitochondria, also plays a role in the deubiquitination of target proteins as component of the BRISC complex: required for IFNAR1 deubiquitination by the BRISC complex (PubMed:24075985).
Alternative names
SHMT, Glycine hydroxymethyltransferase, Serine methylase, SHMT2
Recommended products
Rabbit Polyclonal SHMT2/SHMT antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human Serine hydroxymethyltransferase, mitochondrial aa 400-500.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human Serine hydroxymethyltransferase, mitochondrial aa 400-500. The exact immunogen used to generate this antibody is proprietary information. Database link P34897
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Serine hydroxymethyltransferase 2 (SHMT2) also known as SHMT is an enzyme that plays a role in the mitochondrial compartment. It has a molecular mass of approximately 53 kDa. SHMT2 is highly expressed in tissues with elevated rates of oxidative metabolism such as the liver and brain. The enzyme catalyzes the reversible conversion of serine to glycine with the concomitant production of 510-methylenetetrahydrofolate a critical molecule for DNA synthesis and repair.
- Biological function summary
The enzyme facilitates essential reactions in mitochondrial nucleotide synthesis. SHMT2 operates within a tetrameric complex that provides one-carbon units for cellular biosynthetic processes. The activity of SHMT2 links mitochondrial serine utilization directly to the folate cycle which is essential for the synthesis of purines and thymidylate. The efficient production of these nucleotides is important for rapidly dividing cells supporting their proliferation and maintenance.
- Pathways
SHMT2 takes part in the folate and methionine metabolism pathways. It interacts with proteins like methylenetetrahydrofolate dehydrogenase and thymidylate synthase which are essential for one-carbon metabolism. These interactions integrate SHMT2 activity into a larger network that balances the supply of nucleotides according to the cellular demand impacting vital pathways like DNA replication and repair.
- Associated diseases and disorders
Elevated SHMT2 expression has been linked to cancer where it supports the rapid proliferation of tumor cells by providing necessary nucleotides. Its role in the mitochondrial folate pathway is also significant in neurodegenerative disorders. SHMT2 in conjunction with other proteins such as methionine synthase may play a role in the pathology of diseases like Alzheimer's by influencing homocysteine levels and oxidative stress in brain tissues.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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11 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHMT2/SHMT antibody (ab224428)
Paraffin-embedded human skin tissue stained for SHMT2/SHMT using ab224428 at 1/200 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHMT2/SHMT antibody (ab224428)
Paraffin-embedded human kidney tissue stained for SHMT2/SHMT using ab224428 at 1/200 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHMT2/SHMT antibody (ab224428)
Paraffin-embedded human colon tissue stained for SHMT2/SHMT using ab224428 at 1/200 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHMT2/SHMT antibody (ab224428)
Paraffin-embedded human liver tissue stained for SHMT2/SHMT using ab224428 at 1/200 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHMT2/SHMT antibody (ab224428)
Paraffin-embedded human skeletal muscle tissue stained for SHMT2/SHMT using ab224428 at 1/200 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHMT2/SHMT antibody (ab224428)
Paraffin-embedded human stomach tissue stained for SHMT2/SHMT using ab224428 at 1/200 dilution in immunohistochemical analysis.
Western blot - Anti-SHMT2/SHMT antibody (ab224428)
All lanes: Western blot - Anti-SHMT2/SHMT antibody (ab224428) at 1/100 dilution
Lane 1: RT-4 cell lysate
Lane 2: U-251 MG cell lysate
Predicted band size: 56 kDa
Western blot - Anti-SHMT2/SHMT antibody (ab224428)
All lanes: Western blot - Anti-SHMT2/SHMT antibody (ab224428) at 100 µg
All lanes: RH-30 cell lysate
Predicted band size: 56 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHMT2/SHMT antibody (ab224428)
Paraffin-embedded human lymph node tissue stained for SHMT2/SHMT using ab224428 at 1/200 dilution in immunohistochemical analysis.
Western blot - Anti-SHMT2/SHMT antibody (ab224428)
All lanes: Western blot - Anti-SHMT2/SHMT antibody (ab224428) at 1/100 dilution
Lane 1: NIH/3T3 (mouse embryonic fibroblast cell line) cell lysate
Lane 2: NBT-II cell lysate
Developed using the ECL technique.
Predicted band size: 56 kDa
Immunocytochemistry/ Immunofluorescence - Anti-SHMT2/SHMT antibody (ab224428)
PFA-fixed, Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells stained for SHMT2/SHMT (green) using ab224428 at 4 μg/ml in ICC/IF.
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